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Macrobrachium nipponense MnIAG gene, and amplimer group and amplification method thereof

A primer set, gene technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, genetic engineering, etc.

Inactive Publication Date: 2013-11-20
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Japanese river prawn IAG Genes (named here: QUR ) has not been reported

Method used

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  • Macrobrachium nipponense MnIAG gene, and amplimer group and amplification method thereof
  • Macrobrachium nipponense MnIAG gene, and amplimer group and amplification method thereof
  • Macrobrachium nipponense MnIAG gene, and amplimer group and amplification method thereof

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Embodiment Construction

[0030] Below in conjunction with embodiment, further set forth the present invention. Those skilled in the art can refer to the content of this article to appropriately improve the process parameters to achieve. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.

[0031] Macrobrachium japonicus in the present invention is provided by fishermen in Wuxi Taihu Lake, reagents can be purchased from the market, reagents such as RNA purification are purchased f...

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Abstract

The invention discloses a Macrobrachium nipponense MnIAG gene, and an amplimer group and an amplification method thereof. The full-length cDNA sequence of the MnIAG gene is cloned from the androgenic gland tissue of Macrobrachium nipponense through utilizing the primer group provided by the invention, and an important basis is established for the function of the Macrobrachium nipponense MnIAG gene, especially the important effect in sex differentiation.

Description

technical field [0001] The invention relates to the field of gene cloning, in particular to Macrobrachium japonicus QUR Gene, its amplification primer set and amplification method. Background technique [0002] Androgenic gland (Androgenic gland) is a kind of endocrine gland unique to male crustaceans. In 1947, it was found in blue crab ( Callinectes sapidus ) was discovered for the first time. The androgenic gland secretes androgenic gland hormone, and the androgenic gland is a key endocrine gland in crustaceans that regulates sex differentiation and maintains male secondary sexual characteristics. Transplantation of androgenic glands or injection of androgenic gland extracts into crustacean females masculinizes females; conversely, removal of androgenic glands from males leads to feminization of males. Androgenic gland regulates sex through androgen-stimulating hormone, which is an insulin-like protein hormone (insulin-like androgenic gland hormone, IAG), and its amino...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/16C12N15/11C12N15/10
Inventor 沈怀舜柏爱旭任秀芳周鑫
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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