Molecular marking method for two mutation loci in 5' regulatory region of chicken MMP-11 gene and application in breeding of chicken sexual precocity traits

A technology of MMP-11 and molecular markers, applied in the field of molecular genetics, can solve problems affecting gene expression and transcription initiation, and achieve the effect of reducing feeding costs
CN110205388AActive Publication Date: 2019-09-06SHANDONG AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANDONG AGRICULTURAL UNIVERSITY
Publication Date
2019-09-06

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Abstract

The invention discloses a molecular marking method for two mutation loci in a 5' regulatory region of a chicken MMP-11 gene and an application in breeding of chicken sexual precocity traits. Three mutation loci, namely g.-889 (C>T), g.-636 (A>G) and g.-518 (C>T) loci, are found out to exist in the 5' regulatory region of the chicken MMP-11 gene for the first time, wherein two SNP loci of g.-889 (C>T) and g.-636 (A>G) have significant correlation with the chicken laying starting day age and the egg laying number in the earlier stage of 30 weeks of age; the CA / CA genotype corresponds to the smaller laying starting day age and the higher egg laying number in the earlier stage, and the individual of the TG / TG genotype starts to lay late correspondingly, so that detection of the two molecular marker loci associated with sexual maturity traits is helpful for breeding sexual maturity variety / lines, beneficial help is provided for chicken breeding work, especially for local chicken breeds withlate sexual maturity, and the accurate and effective marker loci are also provided for customization of a molecular marker-assisted breeding chip.
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Description

technical field

[0001] The invention relates to the technical field of molecular genetics, in particular to a molecular marker method for two mutation sites in the 5' regulatory region of the chicken MMP-11 gene and its application in the breeding of chicken precocious puberty traits. Background technique

[0002] Matrix metalloproteinases (MMPs) are a class of proteolytic enzymes dependent on zinc ions, which can degrade extracellular matrix, basement membrane, and interstitial matrix, and play an important role in the development of ovarian follicles. MMPs are involved in extracellular matrix (extracellular matrix) , ECM) is the major protease system for degradation and remodeling (Goldman et al., 2004). MMPs cooperate with their tissue inhibitory factor TIMP and other cytokines to play important regulatory roles in biological processes such as follicle growth, ovulation, atresia and degeneration, and have become a new field of reproductive system research (Littlepage et a...

Claims

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