Method for cultivating arctic charr triploid offspring seeds

A triploid, red dot technology, applied in fish farming, application, climate change adaptation, etc., can solve problems such as chemical sensitivity, high water quality requirements for growth environment, and no 6-dimethylaminopurine.
CN103250667BInactive Publication Date: 2015-01-07BEIJING FISHERIES RES INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING FISHERIES RES INST
Publication Date
2015-01-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for cultivating arctic charr triploid offspring seeds. Oosperms of arctic charrs are placed at 6-8 DEG C, the oosperms are fertilized for 12-14 min, then the oosperms are moved into a 6-DMAP (6-Dimethylaminopurine) solution with concentration being 220-240mg / L and temperature being 9-11 DEG C to be socked for 14-16 min, and the oosperms are moved into a 6-8 DEG C water body to be normally incubated. After about 60 days, fries of the arctic charrs are incubated from the oosperms. During the incubating process, developmental rate of the oosperms is above 61 %, the hatching rate of the fries reaches above 90%, the obtained fries are detected through a flow cytometry, and the triploid rate reaches 79%. According to the method for cultivating the arctic charr triploid offspring seeds, the problems, caused by long-time near relation propagation and shortage of systematic seed selection, of sexual precocity, individual miniaturization, disease breeding and the like in a breeding individual are relieved, and the method has good market value.
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Description

technical field

[0001] The invention relates to the technical field of aquatic organism breeding, in particular to a method for preparing arctic charr triploid seed. Background technique

[0002] Salmon trout is one of the main fish cultured in the world at present. Because of its own quality and special requirements for the living environment, it is a green, environmentally friendly and nutritious food, so it is very popular among consumers. Salmon trout is currently the main cold-water cultured fish in my country. With the rapid development of salmon trout farming in China, basic theoretical research is relatively lagging behind. Problems such as the degradation of broodstock germplasm of salmon trout lead to the degradation of seed quality and the decline in production efficiency have gradually become prominent. , thus restricting the sustainable development of salmon and trout farming. Using modern biotechnology to cultivate seedlings with fast growth, large individuals,...

Claims

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