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Method for cultivating arctic charr triploid offspring seeds

A triploid, red dot technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of sensitivity to chemical substances, high requirements for water quality in the growth environment, and no 6-dimethylaminopurine.

Inactive Publication Date: 2013-08-21
BEIJING FISHERIES RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 6-Dimethylaminopurine (N6-Dimethyladenine, 6-DMAP) has achieved good results in the induction of polyploidy in various shellfish, but because salmon trout have high requirements on the water quality of the growth environment and are particularly sensitive to chemical substances, So far, there is no successful report on the application of 6-dimethylaminopurine in triploid induction of salmon and trout

Method used

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  • Method for cultivating arctic charr triploid offspring seeds
  • Method for cultivating arctic charr triploid offspring seeds

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Method for preparing triploid seedlings of arctic char (1)

[0026] Stimulation by water flow, light control, and enhanced compound feed for broodstock were used to make the broodstock of arctic charr mature. The water temperature for Arctic char broodstock cultivation is 8°C, and the water exchange volume in the flow pool is 2h / time.

[0027] The eggs are fertilized by dry method. Squeeze the roe of the mature female arctic char into a clean porcelain basin first, and then squeeze the semen of 2 male arctic char into the basin. , the water temperature is 8°C, stir immediately, add hatching water with a volume ratio of semen to hatching water of 1:2500, stir gently, pour out the water after 1 min, repeat 3 times, clean the semen, and place it for processing.

[0028] After 12 minutes of fertilization, the fertilized eggs were quickly transferred to the 6-DMAP solution with a concentration of 240mg / L and a temperature of 10°C for soaking treatment. After soak...

Embodiment 2

[0030] Example 2 Preparation method of Arctic char triploid seed (2)

[0031] Stimulation by water flow, light control, and enhanced compound feed for broodstock were used to make the broodstock of arctic charr mature. The water temperature for Arctic char broodstock cultivation is 10°C, and the water exchange volume in the flow pool is 2h / time.

[0032] The eggs are fertilized by dry method. Squeeze the roe of the mature female arctic char into a clean porcelain basin first, and then squeeze the semen of 2 male arctic char into the basin. , the water temperature is 7°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1:3000, stir gently, pour out the water after 1.5 minutes, repeat 3 times, clean the semen, and place it for processing .

[0033] After 13 minutes of fertilization, the fertilized eggs were quickly transferred to 6-DMAP solution with a concentration of 220mg / L and a temperature of 10°C for soaking. After soaking f...

Embodiment 3

[0035] Example 3 Preparation method of Arctic char triploid seed (3)

[0036] Stimulation by water flow, light control, and enhanced compound feed for broodstock were used to make the broodstock of arctic charr mature. The water temperature for Arctic char broodstock cultivation is 9°C, and the water exchange volume in the flow pool is 2h / time.

[0037] The eggs are fertilized by dry method. Squeeze the roe of the mature female arctic char into a clean porcelain basin first, and then squeeze the semen of 2 male arctic char into the basin. , the water temperature is 6°C, stir immediately, add hatching water with a volume ratio of semen to hatching water of 1.5:3000, stir gently, pour out the water after 1 min, repeat 3 times, clean the semen, and place it for processing.

[0038] After 14 minutes of fertilization, the fertilized eggs were quickly transferred to the 6-DMAP solution with a concentration of 240mg / L and a temperature of 9°C for soaking. After soaking for 16 minut...

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Abstract

The invention provides a method for cultivating arctic charr triploid offspring seeds. Oosperms of arctic charrs are placed at 6-8 DEG C, the oosperms are fertilized for 12-14 min, then the oosperms are moved into a 6-DMAP (6-Dimethylaminopurine) solution with concentration being 220-240mg / L and temperature being 9-11 DEG C to be socked for 14-16 min, and the oosperms are moved into a 6-8 DEG C water body to be normally incubated. After about 60 days, fries of the arctic charrs are incubated from the oosperms. During the incubating process, developmental rate of the oosperms is above 61 %, the hatching rate of the fries reaches above 90%, the obtained fries are detected through a flow cytometry, and the triploid rate reaches 79%. According to the method for cultivating the arctic charr triploid offspring seeds, the problems, caused by long-time near relation propagation and shortage of systematic seed selection, of sexual precocity, individual miniaturization, disease breeding and the like in a breeding individual are relieved, and the method has good market value.

Description

technical field [0001] The invention relates to the technical field of aquatic organism breeding, in particular to a method for preparing arctic charr triploid seed. Background technique [0002] Salmon trout is one of the main fish cultured in the world at present. Because of its own quality and special requirements for the living environment, it is a green, environmentally friendly and nutritious food, so it is very popular among consumers. Salmon trout is currently the main cold-water cultured fish in my country. With the rapid development of salmon trout farming in China, basic theoretical research is relatively lagging behind. Problems such as the degradation of broodstock germplasm of salmon trout lead to the degradation of seed quality and the decline in production efficiency have gradually become prominent. , thus restricting the sustainable development of salmon and trout farming. Using modern biotechnology to cultivate seedlings with fast growth, large individuals,...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 徐绍刚李文通田照辉杨晓飞杨贵强
Owner BEIJING FISHERIES RES INST
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