Serum miRNA markers for measuring chicken sexual maturity starting time and preparation method of marker-containing serum

Abstract

The invention relates to serum miRNA markers for measuring a chicken sexual maturity starting time and a preparation method of marker-containing serum. The marker is any one or any combinations of gga-miR-29c-3p, ggamiR-217-5p, gga-miR-375, gga-miR-215-5p, gga-miR-19b-3p, gga-miR-133a-3p and gga-let-7a-5p. By extracting chicken serum small RNA and by detecting the expression law of the miRNA markers in a sexual maturity progress by virtue of a qRT-PCR technology, seven specific cyclic miRNA markers, which are capable of accurately measuring the chicken sexual maturity starting time, are identified; therefore, a more accurate measuring standard for conducting chicken sexual maturity starting mechanism researches and the breeding of a novel chicken line (variety) of sexual precocity is provided.

Description

technical field [0001] The invention relates to a serum miRNA marker for measuring the timing of chicken sexual maturity initiation and a serum preparation method containing the marker, belonging to the technical field of molecular biology. Background technique [0002] Precocious puberty is a pathological condition in humans and an economically important trait in animal production. The onset of sexual maturation (puberty / puberty onset) is a milestone developmental event in the life cycle of animals, including humans, through which the organism acquires reproductive capacity. Research on the regulation of timing of puberty onset has attracted much attention. [0003] In the study of the regulatory mechanism of sexual maturity initiation in chickens and the selection of new strains (breeds) of sexually mature chickens, there is a lack of consistent biomarkers that can accurately measure the timing of sexual maturity initiation. Change indicators such as cockscomb, wattles a...

AI Technical Summary

Problems solved by technology

However, during the course of the study, it was found that if conventional measurement methods ( Observation of phenotypes of secondary sexual characteristics such as cockscomb and whiskers: not completely synchronized with sexual development; Determination of the concentration of reproductive hormones such as LH / FSH: Hormone levels overlap and vary greatly between different developmental stages and different individuals at the same developmental stage) It is difficult to accurately measure the timing of sexual maturity initiation, which has become a bottleneck in the research on the initiation of chicken sexual maturity

In addition, in the production of new strains (breeds) of precocious chickens, it is difficult to carry out early (before cage) precise selection for individuals with precocious genetic characteristics. Seriously lagging behind as a breeding indicator, there is a lack of a biomarker that can assist in early accurate breeding

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