Method for preparing hybrid triploid seeds of golden trout and arctic charr

A triploid and red dot technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of chemical sensitivity, high water quality requirements for growth environment, and absence of 6-dimethylaminopurine

Inactive Publication Date: 2013-09-11
BEIJING FISHERIES RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 6-Dimethylaminopurine (N6-Dimethyladenine, 6-DMAP) has achieved good results in the induction of polyploidy in various shellfish, but because salmon trout have high requirements on the water quality of the growt

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Method for preparing hybrid triploid seed of golden trout and arctic char (1)

[0028] By means of water stimulation, light control, and enhanced compound feed for broodstock, the broodstock of golden trout and broodstock of arctic charr can develop and mature synchronously. The water temperature for broodstock breeding of golden trout was 10°C, and the water exchange volume in the running pool was 2h / time; the broodstock breeding water temperature for arctic char was 8°C, and the water exchange volume in the running pool was 5h / time.

[0029] The eggs are fertilized by dry method. Squeeze the roe of mature golden trout females into a clean porcelain basin first, and then squeeze the semen of 2 male arctic char into a clean porcelain basin. 8°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1:2500, stir gently, pour out the water after 1 min, repeat 3 times, clean the semen, and place it for treatment.

[0030]...

Embodiment 2

[0032] Example 2 Method for preparing hybrid triploid seed of golden trout and arctic char (2)

[0033] By means of water flow stimulation, light control, and enhanced compound feed for broodstock, the broodstock of golden trout and arctic charr can develop and mature synchronously. The water temperature for broodstock breeding of golden trout was 13°C, and the water exchange rate in the running pool was 2h / time.

[0034] The eggs are fertilized by dry method. Squeeze the roe of mature golden trout females into a clean porcelain basin first, and then squeeze the semen of 2 male arctic char into a clean porcelain basin. 8°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1:3000, stir gently, pour out the water after 1.5 minutes, repeat 3 times, clean the semen, and place it for processing.

[0035] After 13 minutes of fertilization, the fertilized eggs were quickly moved into the 6-DMAP solution with a concentration of 170mg / L an...

Embodiment 3

[0037] Example 3 Method for preparing hybrid triploid seed of golden trout and arctic char (3)

[0038]By means of water flow stimulation, light control, and enhanced compound feed for broodstock, the broodstock of golden trout and arctic charr can develop and mature synchronously. The water temperature for broodstock breeding of golden trout was 11°C, and the water exchange rate in the running pool was 2h / time.

[0039] The eggs are fertilized by dry method. Squeeze the roe of mature golden trout females into a clean porcelain basin first, and then squeeze the semen of 2 male arctic char into a clean porcelain basin. 7°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1.5:3000, stir gently, pour out the water after 1 min, repeat 3 times, clean the semen, and place it for processing.

[0040] After 14 minutes of fertilization, the fertilized eggs were quickly transferred to the 6-DMAP solution with a concentration of 190mg / L and...

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PUM

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Abstract

The invention provides a method for preparing hybrid triploid seeds of golden trout and arctic charr. The method includes: subjecting ova of female parent golden trout and sperms of male parent arctic charr to external fertilization, moving fertilized ova to a six-DMAP (N6-dimethyladenine) solution with concentration ranging from 170mg/L to 190mg/L and the temperature ranging from 9 DEG C to 11 DEG C after the fertilized ova are fertilized for 12-14 minutes, soaking for 14-16 minutes prior to moving into water at the temperature of 6-8 DEG C for hatching, and obtaining the hatched hybrid triploid larva fishes from the fertilized ova after about 50 days. During hatching, the eyeing rate of the fertilized ova is larger than 78%, the seed hatching rate reaches more than 90%, the obtained larva fishes are detected by a flow cytometer, and the triploid rate reaches 95%. The method for preparing the hybrid triploid seeds of the golden trout and the arctic charr remits the problems about sexual precocity, size miniaturization, disease breeding and the like of individuals during salmon and trout cultivation, and has the advantages of hybridization and triploid and a good marketing value.

Description

technical field [0001] The invention relates to the technical field of aquatic organism breeding, in particular to a preparation method for hybrid triploid seed of golden trout and Arctic char. Background technique [0002] Salmon trout is one of the main fish cultured in the world at present. Because of its own quality and special requirements for the living environment, it is a green, environmentally friendly and nutritious food, so it is very popular among consumers. Salmon trout is currently the main cold-water cultured fish in my country. With the rapid development of salmon trout farming in China, basic theoretical research is relatively lagging behind. Problems such as the degradation of broodstock germplasm of salmon trout lead to the degradation of seed quality and the decline in production efficiency have gradually become prominent. , thus restricting the sustainable development of salmon and trout farming. Using modern biotechnology to cultivate seedlings with fas...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 徐绍刚李文通田照辉杨晓飞杨贵强
Owner BEIJING FISHERIES RES INST
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