32 results about "Trout/salmon" patented technology

The present invention relates to a gender artificial-control technique of the cultivated fish, in particular to a gender control technique of the all-female-orientation of the salmon and bulltrout. Using the estrogen hormone to feed the normal fry transforms the male fry to be a fake-male fish with the female shape feature and the male physiological feature; using the sperm of the fake-male fish to fertilize the normal egg can realize that all offspring are female. When the fries of the salmon and bulltrout start to take food, the fries are fed with the feed containing methyl testosterone for three to six months and then the fries are fed with normal feed until the sex maturation. Selecting the fake-male fish with a female external morphological feature and a sex gland of the spermarium, taking out the spermarium, grinding the spermarium with a mortar, diluting the spermarium powder with the conventional sperm dilution, nursing the solution inside a refrigerator in four cent degrees for two to four hours, and then mating with the male fish to produce the all-female salmon and bulltrout. All fish output by the present invention are female; when in maturation, the ovary of the fish contains a plurality of fish roes that can be make into a featured dish or processed into the caviar, thereby improving the cultivation benefit.

The invention discloses a salmo trutta fario linnaeus parent fish feed with low protein and amino acid balance, which belongs to the technical field of aquatic feed products, and can be used for solving the problems that no salmo trutta fario linnaeus feed exists and the protein conversion rate is low and the amino acid is unbalanced when a trout feed is used. The salmo trutta fario linnaeus parent fish feed comprises wheat middling, soybean protein concentrate, fish meal, fish oil, soybean soil, cellulose, calcium dihydrogen phoshate, inkfish paste, soyabean lecithin, ethoxyquin, arginine, methionine, phenylalanine and a premix. According to the salmo trutta fario linnaeus parent fish feed, the utilization rate of a feed raw material with lower digestibility of salmo trutta fario linnaeus is reduced, the quality of the salmo trutta fario linnaeus parent fish feed is easily controlled, the digestion utilization rate of the salmo trutta fario linnaeus parent fish feed is improved, a raw material source is stable, the cost is lowered, the problem of unbalanced amino acid of a conventional feed is overcome, the protein efficiency of the feed is favorably improved, the emission of ammonia nitrogen is reduced, the pollution to a water environment is alleviated, the anti-stress ability and the immunity of a salmo trutta fario linnaeus parent fish are improved, and the quality of sperm eggs is improved.

The invention relates to an Atlantic salmon DNA bar code standard detection gene, and primers and application thereof, and belongs to the field of molecular biology. The invention also provides a pair of primers for detecting the Atlantic salmon DNA bar code standard detection gene, and application of an Atlantic salmon DNA bar code standard detection sequence in identifying Atlantic salmons. The primers are used for amplifying Atlantic salmon DNA, an obtained PCR amplification product is subjected to nucleotide sequence determination, a species identification result is judged according to a sequence comparison analysis result, and if the genetic distance between the PCR amplification product and a nucleotide sequence shown in SEQ ID NO: 1 is 0.02 or below, it can be judged that a sample to be detected is Atlantic salmon. According to the method, the Atlantic salmons can be distinguished from other salmons and trout types.

The invention discloses intelligent equipment for artificial insemination of salmon and trout and a use method of the intelligent equipment. The intelligent equipment comprises a placement base, supporting legs, a fixing plate, a placement plate, a limiting plate, a nutrient solution adding device, a movable stirring device and a sperm adding device, wherein the surface of the bottom side of the placement base is fixedly connected to the top ends of the supporting legs, the top side of the left end of the placement base is fixedly connected to the fixing plate, the placement plate is placed on the surface of the top side of the placement base, and the nutrient solution adding device comprises a fixing column, an adding pipe, a valve and an adding nozzle. According to the intelligent equipment for the artificial insemination of the salmon and trout and the using method of the intelligent equipment, the design is novel, the structure is simple, placement trays can be placed in order conveniently, nutrient solutions can be added conveniently, the contact opportunities of sperms and eggs of the salmon and trout are effectively increased, the success rate of insemination and breeding efficiency are improved, meanwhile, the manual operation requirement and labor intensity are greatly reduced through intelligent control, time and labor are saved, and the intelligent equipment for the artificial insemination of the salmon and trout and the using method of the intelligent equipment are easy to popularize and apply in large-scale artificial breeding of the salmon and trout.

The invention provides a salmon trout IHNV monoclonal antibody, which is obtained by secreting a monoclonal hybridoma cell line specific to IHNV N protein produced by immunizing animals with the infectious hematopoietic organ necrosis virus IHNV-BJLL strain. The invention also provides a detection kit and application based on the monoclonal antibody. Among the IHNV monoclonal antibodies obtained in the present invention, the monoclonal antibody secreted by a hybridoma cell line has strong specificity, can detect the infection of domestic U-type and J-type salmon trout IHNV at the same time, and can avoid the occurrence of other rhabdoviruses The cross-reaction overcomes the misdetection and missed detection of IHNV-infected juvenile fish by current molecular biology. The monoclonal antibody secreted by another strain is more specific and can detect U-type salmon trout IHNV infection, but it has no reaction with J-type salmon trout IHNV, so it can be used to identify the genotype of salmon trout IHNV in China. The detection method of the invention can directly observe the result through a fluorescence microscope, and this operation can be completed in a common laboratory, and has a good application prospect.

This application discloses an intelligent equipment for artificial insemination of salmon and trout and its use method, including a placement seat, support legs, a fixed plate, a placement plate, a limit plate, a nutrient solution adding device, a mobile stirring device and a sperm adding device; The bottom side surface of the placement seat is fixedly connected to the top of the support leg, the top side of the left end of the placement seat is fixedly connected with the fixed plate, the top side surface of the placement seat is placed with the placement plate, and the nutrient solution is added The device includes a fixed column, an adding tube, a valve and an adding nozzle. The intelligent equipment for artificial insemination of salmon and trout and its use method are novel in design and simple in structure, which is convenient for orderly placing the placement tray and adding nutrient solution, effectively increasing the chance of contacting sperm and eggs of salmon and trout, thereby improving the chance of insemination Success rate and breeding efficiency, while intelligent control greatly reduces the need for manual operation and labor intensity, saves time and effort, and is easy to promote and apply in large-scale artificial breeding of salmon and trout.

The invention provides a preparation method of game fish triploid fries. The preparation method comprises the following steps of: hybridizing a rainbow trout female parent fish and an arctic char male parent fish; transferring fertilized ova which have been fertilized for 20 minutes under the condition of 7 DEG C into a water body of 26 DEG C and performing heat shock for 20 minutes; then transferring into a water body of 7 DEG C to hatch for about 60 days; and hatching fish fries from the fertilized ova, wherein the eyed rate of the fertilized ova in the hatching period is over 75 percent, the hatching rate of the fries reaches over 90 percent, and the triploid rate of the obtained fries is 90-95 percent through detection of a flow cytometer. According to the preparation method of game fish triploid fries provided by the invention, the problems of sexual precosity, size miniaturization, disease breeding and the like of bred individuals due to long-term close breeding and shortage of systematic breeding in the rainbow trout culturing process are relieved, meanwhile, the hybrid triploid has the two advantages of the hybridization and the triploid, the growth speed and the disease resistance are obviously superior to those of the conventional diploid rainbow trout, and the preparation method has higher economic benefit and market value.

The invention discloses a new water mildew-resistant salmon trout construction method, and relates to a new disease-resistant salmon trout construction method. The method can be used to reduce salmon trout loss caused by water mildew without using a lot of disinfectant or antibiosis medicament, thereby raising salmon trout quality. The method comprises the steps of 1 establishing a basic seed selection group; 2 establishing family systems in the number of N; 3 weeding out; 4 secondary water mildew infection; 5 breeding to establish filial generation family systems in the number of M; and 6 repeating steps 1 to 5 until water mildew-resistant property is stable. According to the invention, beneficial colony heredity variation is utilized, and new disease-resistant species or lines are cultivated through fertile breeding; the new species has good heredity of disease resistance is stable; the appearance of the fish as a goods is prevented from being damaged due to water mildew; and mortality caused by water mildew is reduced to 30% below.

The invention discloses a method for increasing the utilization rate of sperms of sex reversal milter of rainbow trout, relates to a method for increasing the utilization rate of sperms of milter, and aims to solve the problems that the sperm activation rate, the fish egg fertility rate and the eyed rate are low after sperms of sex reversal milter of rainbow trout are treated by using a conventional method. The method comprises the following steps: I, preparing a rainbow trout sex reversal milter semen storage liquid; II, after the semen storage liquid is prepared, adjusting the pH value, and leaving to stand at 8-12 DEG C for later use; III, cutting high-quality and healthy rainbow trout so as to obtain a sex reversal milter spermary, putting the sex reversal milter spermary into a clean mortar without water, grinding the sex reversal milter spermary so as to obtain a mixed semen tissue liquid, filtering the mixed semen tissue liquid by using gauze, mixing the sex reversal milter semen with the semen storage liquid, putting into an oxygen bag, storing, and taking out for fish egg fertilization. The activation rate of the sperms of the sex reversal milter can reach more than 90%, and the fertilization rate and the eyed rate of fish eggs are both more than 70%, therefore, waste of the fish eggs can be greatly reduced. The method is applicable to the field of genetic breeding of the rainbow trout.

The invention provides a production method of Oncorhynchus Mykiss triploid fry. The production method including fertilizing fertilized eggs of Oncorhynchus Mykiss for 12-14 min at the temperature of 7 DEG C to transfer to 240mg / L 6-DMAP (6-Dimethylaminopurine) solution at temperature at 12-14 DEG C for soaking for 14-16 min, and then transferring the fertilized eggs to hatching water to hatch normally. After around 50 days, the Oncorhynchus Mykiss triploid fry are hatched from the fertilized eggs. Eyeing rate of the fertilized eggs during hatching is more than 68%, hatching rate of the fry reaches 90%, the fry are detected by a flow cytometry, rate of triploid reaches 81%. By the aid of the production method of the Oncorhynchus Mykiss triploid fry, the problems of precocious puberty, individual miniaturization, disease breeding and the like on cultivation individuals due to long-term inbreeding and lack of systematic select breeding during cultivation of the Oncorhynchus Mykiss, and the production method has good market value.

The invention relates to the technical field of for cultivating salmon and trout and provides a solid mixed cultivation method for salmon and trout flatfish. The salmon and trout and the flounder are cultivated in a same cultivation pool together. The use ratio of the cultivation water can be greatly improved by the complementary feature of life water of salmon and trout and the flounder, and then the unit water yield and effect are improved; and the maximum economic benefit is realized by using the limited water resource.

The invention discloses a reagent for multiple detection of salmonidae virus and an application thereof. The reagent provided by the invention comprises a specific primer group, wherein the specific primer group comprises at least one pair from a first primer pair to a sixth primer pair; the first primer pair to the sixth primer pair are sequentially primer pairs of: nucleic acid of specifically-amplified spring viraemia of carp virus, nucleic acid of infectious hematopoietic necrosis virus, nucleic acid of viral haemorrhagic septicaemia virus, nucleic acid of infectious salmon anaemia virus,nucleic acid of salmonid alphavirus, and nucleic acid of infectious pancreatic necrosis virus. The upstream and downstream primers of the first primer pair to the sixth primer pair are sequentially the sequences as shown in Seq ID No.1 to Seq ID No.12. The reagent can carry out multiple detection on six salmonidae viruses, is simple and convenient to use, shortens the detection time of the six salmonidae viruses, improves the detection efficiency and quality, and has great significance for rapid detection and prevention and control of the six salmonidae viruses.

The invention discloses a salmon trout cultivating and feeding method, and belongs to the technical field of aquaculture. The technical point of the invention is that the salmon trout cultivation is performed in two sets of sealed recirculating aquaculture systems, and every set comprises 24 culture ponds, and water for cultivation is well water; the cultivation index is that the temperature of water for cultivation is 15-17 DEG C, the dissolving oxygen is 7-8 / mg / L, and water quality indexes of ammonia nitrogen, nitrate, and nitrite are the same condition; the food feeding parameters: the daily feeding amount includes four groups which are 0.7%, 1%, 1.5% and 1.8% of fish weight; the daily feeding frequency is 4 times, and the feeding time is set at 6: 00, 12: 00, 18: 00 and 24: 00; the overall growth condition of the fish is tested once monthly; the survival rate and fatness of fish are calculated; water quality is monitored at fixed time and regular period; the detection position is awater returning pond. The invention is used for salmon trout cultivation.

The invention discloses a biosensing detection method for identification of trout fries. The biosensing detection method is characterized in that on the basis of conservative analysis results of IHNV (infectious hematopoietic necrosis virus) gene sequence from different plant sources in a public database, six specific primers using IHNV virus matrix protein (M) encoding genes as amplification targets are designed, the constant-temperature reverse transcription amplification is performed on the to-be-measured total RNA template of the fish egg or fry sample, and the amplification time is only 15 to 30min; all or part of primers have the electrochemical activity markers in the amplification reaction, one part of dNTP has an anchor marker, the successfully amplified virus M gene sequence simultaneously has the electrochemical activity marker and the anchor marker, the amplified product can be captured onto the electrode surface by the anchor marker, and the carrying of IHNV in the sample can be accurately judged through detecting the electrochemical signal at the surface of the electrode. The biosensing detection method has the advantages that the operation is simple, the reaction is quick, the requirement on equipment precision is low, and the method is suitable for the site quick detection of production and business workplaces of fry farms, culture farms, and the like.

The invention provides a preparation method for a steelhead trout and brook trout hybrid triploid seed. The preparation method includes the steps that a fertilized egg is obtained through hybridization of the steelhead trout and the brook trout; the fertilized egg is soaked in 6-DMAP (dimethylanminopyridine) solution, hatched and cultivated; and the hybrid triploid seed is produced. During incubation period, eyed rate of the fertilized egg is 77%, and hatching rate of the seed reaches above 90%. Then the obtained larvae is detected through a flow cytometry, and triploid rate of the obtained larvae reaches above 90%. With the preparation method, large-scale production of the steelhead trout and brook trout hybrid triploid seed is realized, and growth speed and disease-resistant ability of the trout are enhanced, thereby improving economic efficiency for farmers and bringing good market value.

The invention provides a preparation method of an oncorhynchus mykiss walbaum and oncorhynchus mykiss hybrid triploid offspring seed. The preparation method comprises the steps of: carrying out in vitro fertilization on oncorhynchus mykiss walbaum female parent fish ova and oncorhynchus mykiss male parent fish sperms, transferring into a 170-190 mg / L 6-DMAP (6-dimethylaminopyridine) solution with the temperature of 12-14 DEG C after fertilized ova is fertilized for 12-14minutes, soaking for 14-16minutes, then transferring in a water body with the temperature of 6-8 DEG C and then hatching; hatching the fertilized ovum to obtain hybrid triploid larva fishes after about 50 days; and detecting the larva fishes by using a flow cytometry, wherein the eyed rate of the fertilized ova in a hatching period is above 71 percent, the hatching rate of the seeds reaches above 90 percent and the triploid rate reaches above 80 percent. By using the preparation method of the oncorhynchus mykiss walbaum and oncorhynchus mykiss hybrid triploid offspring seed, the problems of sexual precocity, miniaturization, disease propagation and the like of individuals in a trout cultivating process are solved, the advantages of hybridization and triploid are obtained, and a good market value is achieved.

The invention provides a preparation method for a steelhead trout and arctic char hybrid triploid seed. The preparation method includes the steps that steelhead trout female broodstock egg and arctic char male broodstock sperm are in vitro fertilized; after the fertilized egg is fertilized for 12-14 minutes, the fertilized egg is moved into 6-DMAP (dimethylanminopyridine) solution with concentration of 170-190 milligrams per liter and temperature of 9-11 degrees Celsius to be soaked for 14-16 minutes; and then the soaked fertilized egg is moved into water of the temperature of 6-8 degrees Celsius to be hatched. After about 50 days, a hybrid triploid larvae is hatched by the fertilized egg. During incubation period, eyed rate of the fertilized egg is above 81%, and hatching rate of the seed reaches above 90%. Then the obtained larvae is detected through a flow cytometry, and triploid rate of the obtained larvae reaches 94%. With the preparation method for the steelhead trout and the arctic char hybrid triploid seed, during trout breeding process, problems of precocious puberty, individual miniaturization, disease breeding and the like in an individual fish are alleviated. Furthermore, the preparation method has the advantages of hybrid and triploid and has good market value.