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Male rat fertility comprehensive evaluating method

A comprehensive evaluation and fecundity technology, which is applied in the field of male rat breeding and male rat fecundity identification, can solve problems such as identification errors, and achieve the effects of simple operation, strong pertinence, and reduced feeding costs

Active Publication Date: 2019-11-15
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these indicators are affected by the reproductive status of female mice, resulting in errors in identification

Method used

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  • Male rat fertility comprehensive evaluating method
  • Male rat fertility comprehensive evaluating method
  • Male rat fertility comprehensive evaluating method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] This embodiment is an example of the method for comprehensive evaluation of the fertility of male rats described in the present invention. In this embodiment, the C57BL / 6J male mouse is used as the identification object, and the experiment is repeated 3 times, and 2 male mice of different parents and different batches are used for each repetition, which can illustrate that the present invention does not require surgery, does not need to injure the reproductive organs of male mice, and is effective. Protect high-quality fecundity male rats, and can effectively and objectively identify high-quality male rats. Before female mice and male mice are caged for natural mating, it is necessary to record the estrous state of female mice at 4-5 weeks of growth and development, discard prematurely sexually mature female mice, and test vaginal smears to ensure that female mice are in natural estrus.

[0055] Including the following steps and specific implementation process:

[0056...

Embodiment 2

[0067] This embodiment is a specific embodiment of the evaluation criteria of the method for comprehensive evaluation of the fertility of male rats according to the present invention. This example is the actual operation of detecting the fecundity of C57BL / 6J male mice No. 2, No. 9, No. 10, No. 11, No. 13 and No. 17. The evaluation criteria are included in the data with scores, which are 0 points, 1 point, 2 points and 3 points. 0 points indicate that the observed phenomenon does not match the described phenomenon or the index value is very low; 1 point indicates that the observed phenomenon is similar to the described phenomenon but not obvious or The index value is low; 2 points indicate that the observed phenomenon is consistent with the described phenomenon or the index value is high; 3 points indicate that the observed phenomenon is consistent with the described phenomenon and is particularly obvious or the index value is very high. Make the judging standard table and the...

Embodiment 3

[0091] This embodiment is an example of the fertility effect of the method for comprehensive evaluation of the fertility of male rats and the judging criteria of the present invention. Screen the fecundity of No. 2, No. 9, No. 10, No. 11, No. 13 and No. 17 C57BL / 6J male mice, the mating data of male and female mice, statistics of vaginal status, gestational days, litter size and fetal survival number, explain Positive effect of identification of screened male fertility on production and selection of mice.

[0092] Table 2 Statistical table of judging data of each male mouse

[0093]

[0094]

[0095] It can be seen from the statistical table of the evaluation data of each male mouse in Table 2: record the vaginal status on the second day after mating, as well as the number of days of pregnancy, litter size and fetal survival number; compare the fertility evaluation of male mice in Table 1 according to these four indicators The short list of standards can be judged by sc...

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Abstract

According to a male rat fertility comprehensive evaluating system and a judging criteria thereof, 8-9-week-old male rats and female rats with healthy appearance, sensitive response, up-to-standard weight, bright fur color and well-developed sexual organs are selected respectively and bred in independent cages, the rats freely take breeding feeds and drink water and are bred alternately in light for 10 hours and in darkness for 14 hours, the female rats with sexual precocity or vaginal valgus holes in the 4-5 week are abandoned, the estrus female rats are selected according to vaginal smears, the natural estrus female rats and the selected male rats are put into one cage according to the ratio of 1:1 for mating, vaginal plugs of the female rats are detected 10-12 hours after the female ratsand the male rats are put into one cage, the female rats are put into a quiet environment to be carefully nursed, and the number of pregnancy days, litter size and fetal survival number of the femalerats are counted. The fertility of the male rats can be effectively and accurately authenticated, the high-fertility male rats are screened out, and the high-fertility male rats are effectively protected against surgical injuries. The judging criteria has high pertinence and measurability; through a measurable score comparison criteria table, detection is more analyzable and objective, and the fertility of the male rats is visually judged.

Description

technical field [0001] The invention relates to the technical field of male rat fecundity identification and male rat breeding, in particular to a comprehensive evaluation method for male fecundity. Background technique [0002] The fertility of male mice has a profound impact on the offspring. The quality of male mice directly affects the production and research of mice, so the identification of male fertility plays a crucial role. In production and scientific research, the fecundity of male rats is ignored, and the fecundity of selected male rats is uneven, resulting in a low level of production efficiency, which will also greatly affect the results of scientific research and analysis and judgment. Whether the evaluation methods and criteria of male rat fecundity can accurately, effectively and objectively judge male rat fecundity plays a vital role. [0003] The reproductive system of male mice includes testes, epididymis, vas deferens, prostate and seminal vesicles. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02G06Q10/06
CPCA01K67/02G06Q10/0639
Inventor 孙俊铭欧阳轶强张名媛郭晓萍莫忠香梁宁李家富胡冰刘佳娟何萍
Owner GUANGXI MEDICAL UNIVERSITY
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