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Outstanding fairy mushroom crossbreeding method

A technology of cross-breeding and extracting fairy mushrooms, which is applied in the field of edible fungus breeding, can solve the problems of easy-to-infect bacteria, poor resistance to stress, and long production cycle

Inactive Publication Date: 2018-04-13
JINAN PENGSHENG AGRI TECH
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for crossbreeding of mushrooms with excellent properties in order to overcome the problems of longer production cycle, easy to infect bacteria, and poor stress resistance in the existing industrial cultivation of commonly used mushroom strains. A new variety of true jiji mushroom (Cuixian mushroom) to replace the existing variety

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Embodiment 1

[0042] A method for crossbreeding of mushrooms, the specific steps are as follows:

[0043] (1) Cultivate the white jade mushroom strain and the gray crab mushroom strain respectively to obtain the corresponding age-appropriate mycelia:

[0044]Inoculate it into the PDA medium on the slant of the test tube, culture at 25°C for 10 days, then transfer to the plate PDA medium, culture at 25°C for 5-7 days, then transfer to a 250mL Erlenmeyer flask liquid enriched medium, at 25°C Shake culture on a constant temperature shaker for 4 days, and finally use a sterile pipette tip to pipette 5 mL of fresh young culture solution into another 250 mL Erlenmeyer flask with liquid enriched medium, and culture at a constant temperature of 25°C for 4 days.

[0045] (2) Preparation and regeneration of protoplasts: Under sterile conditions, the age-appropriate mycelia obtained in step (1) were subjected to enzymatic hydrolysis treatment to obtain protoplasts, purified, added to protoplast regene...

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Abstract

The invention discloses an outstanding fairy mushroom crossbreeding method which comprises the following steps that (1) a white jade mushroom bacterial strain and a gray crab mushroom bacterial strainare cultivated respectively to obtain corresponding right-age hyphae; (2) a protoplast is prepared and generated: under the aseptic condition, the right-age hyphae obtained in the step (1) are subjected to enzymolysis treatment respectively, the protoplast is obtained, purification is performed, a protoplast regeneration culture solution is added, and sealing culture is performed; (3) a tip hyphaof the regenerated protoplast is selected for culture, a mononuclear regeneration bacterial strain is screened under the aseptic condition, then the white jade mushroom bacterial strain and the graycrab mushroom bacterial strain are subjected to pairing and crossbreeding, inoculated culture and purification are performed, and the outstanding fairy mushroom is obtained.

Description

technical field [0001] The invention relates to a method for crossbreeding, in particular to a method for crossbreeding mushrooms. The invention belongs to the technical field of edible fungus breeding. Background technique [0002] There are a large number of edible large fungi in nature. Specifically, edible fungi generally refer to edible mushrooms, which can form large fleshy / colloid fruiting bodies or sclerotia-like tissues and can be eaten by humans. Due to the limited varieties of artificially cultivated edible fungi, the cultivation methods of known varieties and the development of new varieties have always been research hotspots in the field of edible fungi. [0003] Shiji mushroom, also known as seafood mushroom, is crisp and tender in texture, delicious in taste, rich in nutrition, and has the taste of sea crab. Since the 1980s, Shiji mushroom has been commercially planted in my country and has become an important edible fungus in the country. One of the factory ...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/40A01G18/50A01H1/02
CPCA01H1/02
Inventor 刘克东刘振涛于秀英杨晶闫卫荣单书凯
Owner JINAN PENGSHENG AGRI TECH
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