New product of edible mushrooms
A new variety, edible fungus technology, applied in the direction of fungal products, applications, fungi, etc., can solve the problems of easy-to-contaminate bacteria, poor stress resistance, long production cycle, etc.
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[0042] A new variety of edible fungus is obtained by cross-breeding through the following methods:
[0043] (1) Cultivate the white jade mushroom strain and the gray crab mushroom strain respectively to obtain the corresponding age-appropriate mycelia:
[0044]Inoculate it into the PDA medium on the slant of the test tube, culture at 25°C for 10 days, then transfer to the plate PDA medium, culture at 25°C for 5-7 days, then transfer to a 250mL Erlenmeyer flask liquid enriched medium, at 25°C Shake culture on a constant temperature shaker for 4 days, and finally use a sterile pipette tip to pipette 5 mL of fresh young culture solution into another 250 mL Erlenmeyer flask with liquid enriched medium, and culture at a constant temperature of 25°C for 4 days.
[0045] (2) Preparation and regeneration of protoplasts: Under sterile conditions, the age-appropriate mycelia obtained in step (1) were subjected to enzymatic hydrolysis treatment to obtain protoplasts, purified, added to p...
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