Method for bolt cytoplasma male sterility line breeding, reproduction andp reparing hybrid seed

A male sterile line and cytoplasmic technology, which is applied in the field of crop genetics and breeding, can solve problems such as emergence, sterile line affected by low temperature, and hybrids not seen in large-scale applications.

Inactive Publication Date: 2005-08-03
NORTHWEST A & F UNIV
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Problems solved by technology

"Chinese Vegetables" (No. 1, 1989: 1-3 and No. 5, 1997: 29-30), and "Guangdong Agricultural Science" (No. 5, 1996: 13-15) respectively reported Peng Qian and Liu Zizhu, etc. Brassica napus is the source of sterility, and the cytoplasmic male sterile lines were selected and bred separately, but the sterile lines bred were affected by the low temperature, and a small amount of pollen appeared, and the first generation of hybrids prepared did not see large-scale application

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  • Method for bolt cytoplasma male sterility line breeding, reproduction andp reparing hybrid seed

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Embodiment Construction

[0017] Beginning in 1989, the inventor of the applicant put the original Chinese cabbage CMS 3411-7 The sterile line (patent number: 92104134.9) is a sterile cytoplasmic donor, and the inbred line of Caishui bred in Liuzhou Zaocai is used as the transgenic male parent, and the CMS 3411-7 The sterility of the Chinese cabbage was transferred to Chinese cabbage, and the cytoplasmic male sterile line TC of cabbage was bred. 1-3-1 and TC 2-8 And corresponding maintenance system TB 1-3-1 and TB 2-8 . Then the first generation of hybrids of cabbage sprouts was cultivated and tested in various places, demonstrated and promoted, showing the characteristics of high quality, high yield, stable yield, multi-resistance, and strong adaptability.

[0018] (1) CMS TC 1-3-1 and TC 2-8 selection process

[0019] 1. In 1989, Guangxi, Guangdong and other places began to collect local varieties of Chinese cabbage, and observed and compared field characteristics such as maturity, yield, dise...

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Abstract

The present invention discloses a method of selectively breeding, reproducing and preparing hybrid seed of Caitai cytoplasm male sterile line. The present invention adopts sterile source Chinese cabbage CMS3411-7 as female parent and Liuzhou Caitai selfed line as male parent, to breed the Caitai cytoplasm male sterile lines TC1-3-1 and TC2-8 through backcross for several generations, pair matching and strict selection. The Caitai cytoplasm male sterile lines TC1-3-1 and TC2-8 have stable sterility, normal pistil function, healthy honey cup, no yellowing in seedling stage and excellent natural pollination. The bred hybrid varieties TC1-3-1x TS14 and TC2-8 x TS46 have fast growth speed, high yield, high quality and high disease resistance.

Description

technical field [0001] The invention relates to the subject of crop genetics and breeding in agricultural science and technology, in particular to a method for selecting a male sterile line of cabbage stalks, the propagation of the selection results and the application in hybrid seed production of cabbage stalks. Background technique [0002] Cabbage sprouts (Brassica campestris L.SSP.chinensis var.utilis Tsen.et.Lee.), also known as cabbage heart. It belongs to Brassicaceae Brassica Brassica species Brassica subspecies, a variety that uses flower sprouts as products, and is a biennial herb. It has a short growth period, fast growth speed and high nutritional value. It is one of the main vegetable species in the annual balanced supply of vegetables in southern my country, and plays an important role in the annual domestic vegetable supply and export trade. In recent years, the planting area in the northern region has increased rapidly and is quite popula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 柯桂兰赵利民
Owner NORTHWEST A & F UNIV
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