Daiquzu pseudosciaena crocea and minyuedongzu cultured pseudosciaena crocea cross breeding method

A hybridization method and technology of large yellow croaker, applied in fish farming, medical science, climate change adaptation, etc., can solve problems such as asynchronous gonad maturation time, and achieve the effect of being suitable for long-distance transportation, strong resistance to stress, and solving isolation

Inactive Publication Date: 2007-04-25
XIAMEN UNIV
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the gonad maturation time of large yellow croaker of Daiqu nationality and Fujian-Guangdongdong population is not synchronized, and to provide a hybrid offspring with fas

Method used

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Embodiment Construction

[0026] 1. Select 2 large yellow croaker males from Fujian, Fujian, Guangdong and Dong nationality with complete body shape, healthy body and soft abdomen, and the ones with milky white semen flowing out from the abdomen are kept for use.

[0027] 2. Prepare sperm diluent. Each 100mL sperm diluent contains: glucose 1.45g; sodium citrate 0.2g; KCL 0.03g; BSA (bovine serum albumin) 3g; adjust the pH to 5.6-6.0.

[0028] 3. Squeeze the abdomen of the male fish, absorb milky white semen without blood and urine, and the sperm motility is 91% after microscopic examination, and keep it for use. The ratio of sperm:diluent:antifreeze is 3:6:1, and the antifreeze uses dimethyl sulfoxide (DMSO).

[0029] 4. Mix the diluent and antifreeze in proportion, then add the sperm, mix thoroughly and store in a 1.8mL cryopreservation tube. After the subpackaged cryopreservation tubes were sealed, they were placed in a 4°C environment to cool and balance for 40 minutes, and then placed above liqui...

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Abstract

The invention relates to a cross method between large yellow Crocker and GuangDong large yellow Crocker. Wherein, their crossed generation has high grow speed and the application in long transmission; the invention selects GuangDong large yellow crocker as father, whose belly can be pressed to flow sperm; preparing dilute solution; compressing its belly to absorb sperm, whose activity is higher than 90% to be frozen and stored; mixing the dilute solution and anti-freeze agent, adding sperm, storing in frozen storage tube, sealing and putting in 4Deg. C; arranging it above the liquid nitrogen, reducing temperature and moving it into liquid nitrogen; selecteing the wide GuangDong large yellow crocker as mother; when its ovogen is mature, defreezing the sperm, and taking the freeze storage tube from liquid nitrogen to be put into room-temperature fresh water to be defrozen, activating it with seawater; artificial inseminating and washing zygote with seawater, removing sink zygote, and charging air and cultivating.

Description

technical field [0001] The invention relates to a large yellow croaker, in particular to a hybridization method of the large yellow croaker of the Daiqu nationality and the cultured large yellow croaker of the Fujian and Guangdong tribes. Background technique [0002] There are three geographical populations of large yellow croaker in the coastal areas of my country, and they have obvious differences in life span, age of sexual maturity, and population age composition. Daiqu large yellow croaker, represented by Daiquyang in northern Zhejiang, has the longest lifespan (up to 29 years old for females and 27 years old for males), and a large number of late sexual maturity (3 years old for males, 3 years old for females and 4 years old for females). age), the population composition is the most complex, and the remaining populations occupy a stable advantage; the Naozhou large yellow croaker, represented by Naozhou in western Guangdong, has the shortest life span (up to 8 years o...

Claims

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Application Information

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IPC IPC(8): A01K61/00A61D19/00
CPCY02A40/81
Inventor 王德祥苏永全王军
Owner XIAMEN UNIV
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