Construction method of Portunus trituberculatus fast-growth strain

The invention relates to a technology of Portunus trituratus and a construction method, which is applied in the field of construction of fast-growing strains of Portunus trituratus, and can solve the problems of long breeding time, slow yield, cumbersome family breeding, etc., so as to speed up the breeding process, alleviate the decline of inbreeding, The effect of avoiding inbreeding decline

Active Publication Date: 2012-05-30
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Due to the large amount of eggs laid by general aquatic animals and the large number of offspring, family selection is very cumbersome and difficult to operate.
Group breeding takes a long time and yields slow results
In recent years, scholars at home and abroad have been paying great attention to molecular marker-assisted breeding, but in practical applications, especially in the genetic breeding of aquatic animals, no results have been achieved.

Method used

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  • Construction method of Portunus trituberculatus fast-growth strain
  • Construction method of Portunus trituberculatus fast-growth strain
  • Construction method of Portunus trituberculatus fast-growth strain

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Experimental program
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Effect test

Embodiment 1

[0017] Using the genomic microsatellites of the breeding materials as molecular markers, the breeding materials are the wild population of Portunus trituratus, using the genomic DNA of the breeding materials as the template, the upstream primer 5'-CTGTCTGATGAGTGAGGCTAC-3' and the downstream primer 5'-TGACCACGAGGAAAGGAG- 3' and upstream primer 5'-GCCACTATCTTGCTGAGGTTGA-3' and downstream primer 5'-GCCATAGCACGAACACTTTTGA-3' two pairs of primers were subjected to double PCR amplification in vitro. The total PCR reaction volume was 25uL, which contained 0.5ul of dNTP (10mM), each Primer 0.2Fmol, 0.4U Taq enzyme, 10×PCR Buffer 2.5ul (containing 1.5mmol Mgcl 2 ). PCR amplification conditions: 94°C for 2 min; then 94°C for 30s, 57°C for 30s, 72°C for 40s, cycle 29 times; and finally 72°C for 10 min. The PCR amplification products are electrophoresed on a 0.7% polyacrylamide gel and developed by silver staining. The genetic background of the individual can be determined by the electro...

Embodiment 2

[0020] Location: Shuangpantu Aquaculture Company, Ninghai County, Zhejiang Province

[0021] In September 2007, at Shuangpantu Aquaculture Company, Ninghai County, Ningbo City, Zhejiang Province, 20 females and 20 males of the Pusatina trituratus were imported into Shandong Laizhou Bay group, and 20 females and 20 males were imported into Nan'ao Island, Guangdong at the same time. Only, put it in the holding tank for temporary maintenance. Then, the females and males of the two groups were crossed to construct 40 groups of crosses. The egg-bearing crabs were temporarily raised until April 2008, and the surviving individuals began to hatch larvae one after another. After conventional artificial breeding, a total of 0.75kg of seedlings C1-C2 emerged in June 2008, and were placed in two ponds for breeding. In October 2008, the offspring adults of the hybrid combination were captured in Shuangpantu Aquaculture Company, Ninghai County, Ningbo City, Zhejiang Province, and various m...

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Abstract

The invention relates to the field of aquatic product inheritance breeding, in particular to a construction method of Portunus trituberculatus fast-growth strain. The genome microsatellite of breeding materials are utilized to serve as molecular markers; according to the molecular marker, obtaining a group which has obvious reproductive isolation in hereditary constitution as a hybridization group, and carrying out hybridized combination to the hybridization group to obtain the Portunus trituberculatus fast-growth strain. The invention can utilize the species genetic resources to the utmost, which is favorable for more scientifically cultivating the Portunus trituberculatus fast-growth strain.

Description

technical field [0001] The invention relates to the field of aquatic genetics and breeding, in particular to a method for constructing a fast-growing strain of Portunus trituratus. Background technique [0002] The genetic breeding of aquatic animals is traditionally carried out by the method of group selection or crossbreeding. At present, there are also attempts to learn from the method of family selection and breeding used in poultry storage. Due to the large number of eggs laid and the large number of offspring of general aquatic animals, family breeding is very cumbersome and difficult to operate. Group breeding takes a long time and the effect is slow. In recent years, scholars at home and abroad have been paying great attention to molecular marker-assisted breeding, but it has not been effective in practical application, especially in the genetic breeding of aquatic animals. SUMMARY OF THE INVENTION [0003] The object of the present invention is to provide a fast...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A01H1/02
Inventor 崔朝霞谭烽王春琳吴丹华栾维莎
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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