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Method of obtaining oyster distant intergeneric hybrid

A technology of distant hybridization and hybrids, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of no oyster interspecific hybridization research report and the non-viability of interspecific hybrids.

Active Publication Date: 2015-05-20
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

More importantly, they proposed that interspecific hybrids within the genus Pyrex were non-viable, which resulted in almost no reports of interspecific hybrids in oysters for about 15 years.

Method used

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  • Method of obtaining oyster distant intergeneric hybrid
  • Method of obtaining oyster distant intergeneric hybrid
  • Method of obtaining oyster distant intergeneric hybrid

Examples

Experimental program
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Embodiment 1

[0021] a. Collection of parents: at the beginning of April 2013, according to the technical route figure 1 , collected in Dongchong, Shenzhen, the Oyster genus Portuguese oyster, and an oyster with many small teeth on the inner edge of the shell. After identification, it is a monk's cap oyster of the genus Oyster ( figure 2 ). There is no difference between the two oysters in appearance, but when the shell is opened, the monk's cap oyster can be distinguished, while the Portuguese oyster still needs molecular identification. Through composite COI molecular identification, Portuguese oyster will produce 222bp specific fragment ( image 3 , WangH, GuoX. (2008) Identification of Crassostrea ariakensis and related doysters by multiplex species-specific PCR. JShellfishRes27:481–487). For the parents, first number them, and then refer to the invention patent technology disclosed by Yu Ruihai & Wang Zhaoping (authorized announcement number: CN101849520B) to carry out live sampling...

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Abstract

The invention discloses a method of obtaining an oyster distant intergeneric hybrid. The method includes a step of collecting different intergeneric parents, labeling each identified species and synchronously ripening; a step of, after the gonads of the parents are ripe, dissecting sperms and eggs, isolating strictly and performing intergeneric hybridization and matching to obtain a selfing group and a hybridization filial generation group of two parents, and a step of cultivating the filial generation to obtain the survival distant intergeneric hybrid. At the same time, the type of the hybrid is determined by performing heredity identification by utilization of molecule and adoption of cytology means. According to the method, oyster intergeneric hybridization is performed for the first time and the survival intergeneric hybrid is obtained, thus providing a rare scarce material for analysis of oyster genetic differentiation, reproductive isolation and species formation and developing a novel route for genetic breeding of the oyster. The method has advantages of simple operation, high feasibility, and the like.

Description

Technical field: [0001] The invention belongs to the technical field of shellfish genetics and breeding in modern marine agriculture, and in particular relates to a method for obtaining distant hybrids between oyster genera. Background technique: [0002] Intergeneric hybridization is the hybridization between species of different genus within the same family. It can significantly expand the gene pool of animals and plants, promote interspecific communication, introduce beneficial genes of different species, create unprecedented variation types, and even synthesize new species ( Lou Yundong, 1999). [0003] For aquatic animals, intergeneric hybridization has mainly focused on fish breeding, and relatively little research has been done on shellfish. Among them, the relatively successful one is the distant hybridization of Chlamys farreri and Ezo scallop (Yang Aiguo et al., 2003), which has obtained a hybrid group with growth advantages and achieved relatively ideal economic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 张跃环苏家齐肖述喻子牛
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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