Maize reproductive isolation method
A technology for reproductive isolation and corn, applied in the directions of botanical equipment and methods, applications, and plant genetic improvement, can solve problems such as high cost, poor reproductive isolation technology, and difficulty in achieving reproductive isolation of corn varieties.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0017] 1. In the summer of 2008, Datian, Tai'an, Shandong: crossed with HN287 as the female parent and SDGa25 (Gal-S) as the male parent (both female and male parents were independently bred by the applicant), and obtained F 1 ;
[0018] 2. In the autumn of 2008, Datian, Ledong County, Hainan: with F 1 As the male parent, use HN287 as the reincarnation parent (female parent), carry out backcrossing, and obtain BC 1 f 1 seed;
[0019] 3. In the spring of 2009, Shandong Taian Greenhouse: the BC 1 f 1 Individual plants of the isolated population were listed and marked, and the listed individual plants were used as male parents, and the recurrent parent HN287 and the donor parent SDGa25 were pollinated respectively, and the pollinated individual plants were marked one by one (with BC 1 f 1 The markers of the individual plants of the segregated populations were consistent). When harvesting, first check the seed setting of the donor parent SDGa25, and only harvest the recurre...
Embodiment 2
[0024] 1. In the summer of 2008, Datian, Tai'an, Shandong: crossed with HN287 as the female parent and SDGa25 (Gal-S) as the male parent (both female and male parents were independently bred by the applicant), and obtained F 1 ;
[0025] 2. In the autumn of 2008, Datian, Ledong County, Hainan: with F 1 As the male parent, use HN287 as the reincarnation parent (female parent), carry out backcrossing, and obtain BC 1 f 1 seed;
[0026] 3. In the spring of 2009, Shandong Taian Greenhouse: the BC 1 f 1 List and mark individual plants, collect leaves, analyze genotypes indoors, select individuals with markers closely linked to the Gal-S gene as male parents, and continue to backcross with recurrent parents to obtain BC 2 f 1 seed. The molecular marker used for indoor genotype analysis was Ac214441Est-3, and its sequence was: forward primer: 5'-GAGAGCTACGCACGACTTAT-3', reverse primer: 5'-CAAGACTTGCACAATCGAGG-3'. Using leaf DNA as a template for PCR amplification, the length ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com