Maize reproductive isolation method

A technology for reproductive isolation and corn, applied in the directions of botanical equipment and methods, applications, and plant genetic improvement, can solve problems such as high cost, poor reproductive isolation technology, and difficulty in achieving reproductive isolation of corn varieties.

Inactive Publication Date: 2012-10-17
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to provide a method for reproductive isolation of corn in view of the poor effect and high cost of the existing corn reproductive isolation technology, which makes it difficult to realize reproductive isolation among corn varieties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Maize reproductive isolation method
  • Maize reproductive isolation method
  • Maize reproductive isolation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. In the summer of 2008, Datian, Tai'an, Shandong: crossed with HN287 as the female parent and SDGa25 (Gal-S) as the male parent (both female and male parents were independently bred by the applicant), and obtained F 1 ;

[0018] 2. In the autumn of 2008, Datian, Ledong County, Hainan: with F 1 As the male parent, use HN287 as the reincarnation parent (female parent), carry out backcrossing, and obtain BC 1 f 1 seed;

[0019] 3. In the spring of 2009, Shandong Taian Greenhouse: the BC 1 f 1 Individual plants of the isolated population were listed and marked, and the listed individual plants were used as male parents, and the recurrent parent HN287 and the donor parent SDGa25 were pollinated respectively, and the pollinated individual plants were marked one by one (with BC 1 f 1 The markers of the individual plants of the segregated populations were consistent). When harvesting, first check the seed setting of the donor parent SDGa25, and only harvest the recurre...

Embodiment 2

[0024] 1. In the summer of 2008, Datian, Tai'an, Shandong: crossed with HN287 as the female parent and SDGa25 (Gal-S) as the male parent (both female and male parents were independently bred by the applicant), and obtained F 1 ;

[0025] 2. In the autumn of 2008, Datian, Ledong County, Hainan: with F 1 As the male parent, use HN287 as the reincarnation parent (female parent), carry out backcrossing, and obtain BC 1 f 1 seed;

[0026] 3. In the spring of 2009, Shandong Taian Greenhouse: the BC 1 f 1 List and mark individual plants, collect leaves, analyze genotypes indoors, select individuals with markers closely linked to the Gal-S gene as male parents, and continue to backcross with recurrent parents to obtain BC 2 f 1 seed. The molecular marker used for indoor genotype analysis was Ac214441Est-3, and its sequence was: forward primer: 5'-GAGAGCTACGCACGACTTAT-3', reverse primer: 5'-CAAGACTTGCACAATCGAGG-3'. Using leaf DNA as a template for PCR amplification, the length ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a maize reproductive isolation method. According to the invention, maize inter-variety reproductive isolation is realized through a maize hybridization unidirectional incompatibility gene. The maize hybridization unidirectional incompatibility gene is Gal-S or a gene having functions same with or similar to that of the Gal-S gene. The maize containing the Gal-S gene does not accept pollens of other maize which does not contain the gene. Even if different varieties are planted alternately, the maize is not influenced by pollens of other varieties. With the method, inherent defects of existing time reproductive isolation, space reproductive isolation and natural barrier reproductive isolation can be completely solved.

Description

technical field [0001] The invention relates to a new method for maize reproductive isolation. Background technique [0002] Crops can be divided into three categories according to the pollination method: self-pollination, cross-pollination and normal cross-pollination. Maize is monoecious and has different flowers, which is a typical cross-pollination crop. Therefore, in the production of corn hybrids and the production of special corn such as sweet corn and waxy corn, reproductive isolation must be carried out to avoid pollen transmission between different varieties, resulting in pollute. [0003] In order to avoid pollen transmission among maize varieties, cause pollution, and realize reproductive isolation, the following three reproductive isolation techniques are mainly adopted. [0004] Spatial reproductive isolation means that the distance between corn varieties is at least 300 meters, and the spread of pollen can be avoided through physical distance. Spatial isola...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 陈化榜肖必祥赵丽
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap