Establishment of a sperm freezer for grouper serrata and method for assisting grouper distant hybrid breeding

A saddle grouper, distant hybridization technology, applied in fish farming, preservation of human or animal body, climate change adaptation, etc. To achieve the effect of improving the probability of success and expanding the scope
CN106417113BActive Publication Date: 2019-10-25YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Publication Date
2019-10-25

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Abstract

The invention discloses a method for establishing a giant grouper sperm freezer and assisting grouper distant hybridization breeding. The sperm freezer establishing method comprises the steps of preparing sperm diluent, preparing sperm cryopreservation liquid, freezing and storing sperms and establishing the sperm freezer. The grouper distant hybridization breeding method comprises the steps of establishing the giant grouper sperm freezer, establishing saladfish breeding groups, cultivating saladfish parent fish and performing reproductive regulation, performing artificially spawning breeding, fertilizing the frozen sperms and performing incubation, and cultivating fries. According to the method, the first giant grouper sperm freezer is established in China, reproductive isolation of the groupers caused by factors, such as geographical distribution, optimum temperature range and breeding time, is overcome, and distant hybridization breeding of the grouper groups is possible, so that distant hybridization breeding varieties are cultivated.
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Description

technical field

[0001] The invention belongs to the technical field of fish breeding, and in particular relates to a method for establishing a sperm freezing bank of saddleband grouper and a method for using frozen sperm to assist grouper distant hybrid breeding. Background technique

[0002] Grouper is an important economic fish and excellent germplasm resource for marine aquaculture in my country. There are 36 species of grouper in China, mainly distributed in the East China Sea and South China Sea. In 2014, the grouper fishing volume reached 113,096 tons, and the breeding volume reached 88,130 tons, which played an important role in the development of blue agriculture. In recent years, with the rapid development of the marine aquaculture industry, this rare fish species has begun to be cultured in large quantities in the north and south of my country, and the demand for fry is hundreds of millions. At present, there are more than 10 species of grouper cultured at home an...

Claims

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