Establishment of a sperm freezer for grouper serrata and method for assisting grouper distant hybrid breeding

A saddle grouper, distant hybridization technology, applied in fish farming, preservation of human or animal body, climate change adaptation, etc. To achieve the effect of improving the probability of success and expanding the scope

Active Publication Date: 2019-10-25
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0006] The invention relates to a method for establishing a sperm freezer of saddletail grouper and a method for assisting grouper distant hybrid breeding with frozen sperm, which solves the problems of small number of male fish, asynchronous maturity and insufficient semen volume in artificial breeding of grouper problem, overcome the reproductive isolation between grouper species caused by factors such as geographical distribution, breeding time, and breeding temperature, thereby realizing the distant hybrid breeding between different species of grouper, and cultivating species with excellent growth characteristics. New species of grouper

Method used

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  • Establishment of a sperm freezer for grouper serrata and method for assisting grouper distant hybrid breeding
  • Establishment of a sperm freezer for grouper serrata and method for assisting grouper distant hybrid breeding
  • Establishment of a sperm freezer for grouper serrata and method for assisting grouper distant hybrid breeding

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Embodiment 1

[0055] 1. Establishment of a cryobank for saddle grouper sperm

[0056] (1) Preparation of sperm diluent (ELRS3): use glucose 49.97g / L, NaCl 6.5g / L, NaHCO 3 0.199g / L, KCl 0.14g / L, CaCl 2 2H 2 O 0.12g / L, NaH 2 PO 4 0.0098g / L, prepare sperm diluent ELRS3 in distilled water, its osmotic pressure is 400mOsm / L, pH value is 7.03.

[0057] (2) Preparation of sperm cryopreservation solution: add 15% dimethyl sulfoxide (DMSO) and 10% calf serum (FBS) to the sperm diluent ELRS3 to prepare a sperm cryopreservation solution, and place it in a 4°C refrigerator for later use , both are parts by volume.

[0058](3) Sperm cryopreservation: During the sexual maturity season of saddle grouper (from April to July every year), spermatozoa of saddle grouper were carried out at Lingshui grouper farm in Sanya, Hainan Province and Laizhou Mingbo Aquatic Products Co., Ltd., Shandong Province. For cryopreservation, the sexually mature grouper was anesthetized with 50ppm eugenol, and the sperm was...

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Abstract

The invention discloses a method for establishing a giant grouper sperm freezer and assisting grouper distant hybridization breeding. The sperm freezer establishing method comprises the steps of preparing sperm diluent, preparing sperm cryopreservation liquid, freezing and storing sperms and establishing the sperm freezer. The grouper distant hybridization breeding method comprises the steps of establishing the giant grouper sperm freezer, establishing saladfish breeding groups, cultivating saladfish parent fish and performing reproductive regulation, performing artificially spawning breeding, fertilizing the frozen sperms and performing incubation, and cultivating fries. According to the method, the first giant grouper sperm freezer is established in China, reproductive isolation of the groupers caused by factors, such as geographical distribution, optimum temperature range and breeding time, is overcome, and distant hybridization breeding of the grouper groups is possible, so that distant hybridization breeding varieties are cultivated.

Description

technical field [0001] The invention belongs to the technical field of fish breeding, and in particular relates to a method for establishing a sperm freezing bank of saddleband grouper and a method for using frozen sperm to assist grouper distant hybrid breeding. Background technique [0002] Grouper is an important economic fish and excellent germplasm resource for marine aquaculture in my country. There are 36 species of grouper in China, mainly distributed in the East China Sea and South China Sea. In 2014, the grouper fishing volume reached 113,096 tons, and the breeding volume reached 88,130 tons, which played an important role in the development of blue agriculture. In recent years, with the rapid development of the marine aquaculture industry, this rare fish species has begun to be cultured in large quantities in the north and south of my country, and the demand for fry is hundreds of millions. At present, there are more than 10 species of grouper cultured at home an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10A01N1/02
CPCA01K61/00A01N1/0221Y02A40/81
Inventor 田永胜翟介明陈张帆马文辉段会敏唐江刘江春侯云霞庞尊方孙正祥李文升李波
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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