New method for mapping corn one-way outcross incompatibility gene

A unidirectional outcrossing and corn technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as limiting the size of the positioning group, save manpower and material resources, improve positioning efficiency, and avoid positioning deviation Effect

Active Publication Date: 2013-10-16
渤海粮仓南皮种业有限公司
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  • Abstract
  • Description
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Problems solved by technology

[0012] The present invention aims at the disadvantages that the existing maize Ga gene localization phenotype identification is easily affected by human and environmental f

Method used

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  • New method for mapping corn one-way outcross incompatibility gene
  • New method for mapping corn one-way outcross incompatibility gene
  • New method for mapping corn one-way outcross incompatibility gene

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Embodiment Construction

[0020] Through the positioning process of Ga1-s gene, the present invention is described in detail:

[0021] (1) In the winter of 2011, at the Lingshui South Breeding Base in Hainan, popcorn SDGa25(Ga1-s / Ga1-s) was used as the male parent, B73(ga1 / ga1) was used as the female parent, and F 1 (Ga1-s / ga1).

[0022] (2) In the summer of 2012, Beijing Changping Farm, with F 1 SDGa25 was pollinated and 50,000 seeds (Ga1-s / Ga1-s) were harvested.

[0023] (3) DNA was extracted from 50,000 individual seeds.

[0024] (4) To screen the polymorphic and band-specific molecular markers of maize chromosome 4 between SDGa25 and B73.

[0025] (5) Among the 50,000 DNAs mentioned above, 2,000 DNAs were randomly selected and tested with the screened molecular markers. Several molecular markers closely linked to the Ga1-s gene were found by using the two-point test method: umc1758, SD1, SD2, SD3, SD12, SD14, and umc1294 and the molecular markers that are completely linked to the Ga1-s gene: SD...

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Abstract

The invention belongs to the technical field of corn gene mapping, and particularly to a new method for mapping corn one-way outcross incompatibility gene. The conventional method for mapping qualitative character gene is constructing a backcrossing or self-crossing population, inferring a genotype of an individual through phenotypic identification, carrying out molecular marker detection, and adopting two point and three point tests to determine a position of the gene, wherein phenotypic identification is easily affected by human factors and environmental factors and the mapped population size is limited due to large workload when the conventional method is used to map the Ga gene. According to the present invention, based on the disadvantages of Ga gene mapping of the conventional method, a mapping method without phenotypic identification is provided, and the method is characterized in that the genotype of the utilized mapped population is known, the phenotype does not require identification, the large population can be constructed, and sufficient individuals can be found in the one generation so as to carry out fine mapping on the Ga gene; and an effective method for Ga gene mapping is provided, and application of the Ga gene to carry out reproduction separation on different types of corn on the production is promoted.

Description

technical field [0001] The invention belongs to the technical field of maize gene positioning, and in particular relates to a new method for locating unidirectional outcross incompatibility genes in maize. Background technique [0002] Unidirectional outcross incompatibility in maize is usually controlled by a single dominant gene, which is a gametophyte factor (Ga). Ga gene refers to the genetic factor that affects the sexual transmission of haploid gametes. Many popcorns with the Ga gene can normally pollinate dent and durum corn without the Ga gene (ga), but backcrosses are often unsuccessful. Generally speaking, ga pollen cannot pollinate the dominant homozygous Ga / Ga female parent, but Ga pollen can pollinate the recessive homozygous ga / ga female parent. Pay incompatibility. At present, multiple Ga genes have been found, and they are located on different chromosomes of maize (Chr1: Ga4 and Ga6; Chr2: Ga7; Chr4: Ga1 and Tcb1; Chr5: Ga2 and Ga10; Chr6: Ga3; Chr9: Ga8) ...

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 陈化榜赵贤容刘旭
Owner 渤海粮仓南皮种业有限公司
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