Construction method of Portunus trituberculatus fast-growth strain

The invention relates to a technology for the construction of Portunus trituratus and a construction method, which is applied to the field of construction of fast-growing strains of Portunus trituratus, and can solve the problems of cumbersome family selection, long selection time, large number of offspring, etc., so as to avoid inbreeding decline and speed up the breeding process. , the effect of clear genetic background

Active Publication Date: 2010-06-16
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

Due to the large amount of eggs laid by general aquatic animals and the large number of offspring, family selection is very cumbersome and difficult to operate.
Group breeding takes a long time and yields slow results
In recent years, scholars at home and abroad have been paying great attention to molecular marker-assisted breeding, but in practical applications, especially in the genetic breeding of aquatic animals, no results have been achieved.

Method used

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  • Construction method of Portunus trituberculatus fast-growth strain
  • Construction method of Portunus trituberculatus fast-growth strain
  • Construction method of Portunus trituberculatus fast-growth strain

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Embodiment 1

[0017] The genomic microsatellites of the breeding materials were used as molecular markers, and the breeding materials were wild populations of Portunus trituberculatus. The genomic DNA of the breeding materials was used as a template, and the upstream primer 5'-CTGTCTGATGAGTGAGGCTAC-3' and the downstream primer 5'-TGACCACGAGGAAAGGAG- 3'and the upstream primer 5'-GCCACTATCTTGCTGAGGTTGA-3'and the downstream primer 5'-GCCATAGCACGAACACTTTTGA-3'two pairs of primers for double PCR amplification in vitro. The total PCR reaction volume is 25uL, which contains 0.5ul of dNTP (10mM), each Primer 0.2Fmol, 0.4U Taq enzyme, 10×PCR Buffer2.5ul (containing 1.5mmol Mgcl 2 ). PCR amplification conditions: 94°C for 2min; then 94°C for 30s, 57°C for 30s, 72°C for 40s, cycle 29 times; finally 72°C for 10min. PCR amplified products were electrophoresed on 0.7% polyacrylamide gel and visualized by silver staining. The genetic background of the individual could be determined through the electropho...

Embodiment 2

[0020] Location: Shuangpantu Aquaculture Company, Ninghai County, Zhejiang Province

[0021] In September 2007, 20 female individuals and 20 male individuals of Portunus trituberculatus Shandong Laizhou Bay population were imported into Shuangpantu Aquaculture Company of Ninghai County, Ningbo City, Zhejiang Province. At the same time, 20 female individuals and 20 male individuals were imported into Nan’ao Island population of Guangdong Only, put them in the holding pool for holding. Then, the female individuals and male individuals of the two populations were crossed to construct 40 groups of hybrid combinations. The egg-bearing crabs were temporarily raised until April 2008, and the surviving individuals began to hatch larvae one after another. After conventional artificial breeding, a total of 0.75kg of seedlings in the C1-C2 stage emerged in June 2008, and they were placed in 2 ponds for breeding. In October 2008, the offspring of the hybrid combination were captured at S...

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Abstract

The invention relates to the field of aquatic product inheritance breeding, in particular to a construction method of Portunus trituberculatus fast-growth strain. The genome microsatellite of breeding materials are utilized to serve as molecular markers; according to the molecular marker, obtaining a group which has obvious reproductive isolation in hereditary constitution as a hybridization group, and carrying out hybridized combination to the hybridization group to obtain the Portunus trituberculatus fast-growth strain. The invention can utilize the species genetic resources to the utmost, which is favorable for more scientifically cultivating the Portunus trituberculatus fast-growth strain.

Description

technical field [0001] The invention relates to the field of aquatic genetics and breeding, in particular to a method for constructing a fast-growing strain of Portunus trituberculatus. Background technique [0002] The genetic breeding of aquatic animals is traditionally carried out by group selection or crossbreeding. At present, there are also attempts to learn from the method of family selection used in poultry storage. Due to the large amount of eggs laid by general aquatic animals and the large number of offspring, family selection and breeding are very cumbersome and difficult to operate. Group selection takes a long time and has slow results. In recent years, scholars at home and abroad have been paying great attention to molecular marker-assisted breeding, but in practical applications, especially in the genetic breeding of aquatic animals, no results have been achieved. Contents of the invention [0003] The object of the present invention is to provide a fast,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A01H1/02
Inventor 崔朝霞谭烽王春琳吴丹华栾维莎
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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