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A kind of artificial hybrid breeding method of fast-growing grouper

An artificial hybridization and grouper technology, applied in fish farming, application, climate change adaptation and other directions, can solve the problems of grouper hybrid breeding difficulties, differences in sexual maturity time, and geographical distribution, etc., and achieve excellent appearance and traits. , fast growth, large individual effect

Active Publication Date: 2021-02-12
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The geographical distribution of different groupers is different, and the time of sexual maturity is different, which brings great difficulties to the cross-breeding of grouper

Method used

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  • A kind of artificial hybrid breeding method of fast-growing grouper
  • A kind of artificial hybrid breeding method of fast-growing grouper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The 2018 cross-breeding of red-spotted grouper and blue-bodied grouper includes the following steps:

[0046] (1) Parent selection

[0047] a. Red-spotted grouper female parent: choose the red-spotted grouper with normal body size, no disease, and good vitality as the female parent above the age of 4;

[0048] b. Male parent of the blue-bodied grouper: select the female fish of the blue-bodied grouper who are over 8 years old, normal in size, disease-free, and active, and then turn into males through sexual reversal.

[0049] (2) Cage cultivation of red-spotted grouper broodstock

[0050]a. Cultivation environment: the water temperature is not lower than 17°C, the seawater salinity is 28, the pH is 7-9, the water flow is 0.3-0.8m / s, the water depth is 5-7m, and the maximum wind wave is 6. The cage size is 3m×3m×3m, and the breeding density is 200 fish / box.

[0051] b. Cultivation in non-breeding seasons

[0052] Feed once a day. The bait is mainly fresh small miscel...

Embodiment 2

[0070] Comparative breeding of hybrid fry and purebred red grouper fry in 2018

[0071] In April 2018, the cross-breeding of red-spotted grouper and blue-spotted grouper was carried out, and the breeding of pure-bred red-spotted grouper was carried out as a comparison. The specific operation methods such as obtaining and hatching of hybrid fertilized eggs are the same as embodiment 1, and the semen used in the breeding of purebred red grouper fry of the control group is fresh semen, and the hatching of fertilized eggs is the same as that of hybrids. 10 mL of frozen semen of the blue-bodied grouper was used to fertilize the eggs of the red-spotted grouper, and 4 kg of fertilized eggs were obtained, while the control group obtained 3.8 kg of fertilized eggs. There was no significant difference in the fertilization rate and hatching rate between the hybrid and red-spotted grouper. In the factory breeding workshop, the water temperature is 25°C, the dissolved oxygen is 6mg / L, the...

Embodiment 3

[0075] Comparative breeding of hybrid fry and purebred red grouper fry in 2019

[0076] The specific operation methods such as obtaining and hatching of hybrid fertilized eggs are the same as embodiment 1, and the semen used in the breeding of purebred red grouper fry of the control group is fresh semen, and the hatching of fertilized eggs is the same as that of hybrids. 8mL of the frozen semen of the blue-bodied grouper was used to hybridize with the eggs of the red-spotted grouper, and 2.8 kg of fertilized eggs were obtained, while the control group obtained 3.2 kg of fertilized eggs. The hatching rate of fertilized eggs of hybrid species was 66.5%, and that of purebred red-spotted grouper was 70.2%. Measure and count the hybrid and purebred red-spotted grouper of 1-77 days old ( figure 1 ). When cultivated to 77 days old, the average length of the hybrids was (49.6±3.5) mm, and the average length of the red-spotted grouper was (43.4±4.1) mm. When cultivated to 3-5cm, the...

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Abstract

The invention relates to an artificial hybridization breeding method for groupers high in growth speed, and belongs to the technical field of aquaculture. The method comprises the steps of parent selection, cage culture of parent red-spotted groupers, industrial culture of parent epinephelus tukula, sexual transformation of the epinephelus tukula, freeze preservation of seminal fluid of the epinephelus tukula, spawning induction of the parent red-spotted groupers, artificial insemination and hatching of fertilized eggs. By using the sperm freeze preservation technology, an epinephelus tukula sperm bank is built, reproductive isolation between the two kinds of groupers is broken through, and a new excellent hybrid variety which is high in growth speed is obtained. The problem is solved thatmale parent epinephelus tukula produces fewer sperms, and consequently, production is hindered, the productivity is greatly improved, and grouper breeding work is facilitated.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to an artificial hybrid breeding method for fast-growing grouper. Background technique [0002] Grouper is an important marine economic fish in my country. In recent years, the marine aquaculture industry has developed rapidly, and the grouper aquaculture industry has also flourished, spreading across the northern and southern coastal areas of China. However, there are some difficulties in the breeding process of grouper, such as seed cultivation, high deformity rate, many diseases and the degradation of germplasm resources. In order to promote the healthy and sustainable development of the grouper aquaculture industry, hybrid breeding as a new species cultivation method has been applied by domestic and foreign scholars and aquaculture enterprises. [0003] The red-spotted grouper (Epinephelus akaara) and the blue-spotted grouper (Epinephelus tukula) both belong to the order P...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 田永胜李振通吴玉萍张晶晶李子奇王林娜
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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