Method for breaking self-incompatibility of apple to obtain filial generation

An affinity, apple technology, applied in the field of plant biology, can solve the problem that apples are difficult to obtain allelic self-bred progeny, and achieve the effect of improving efficiency

Inactive Publication Date: 2019-06-14
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This reproductive isolation mainly brings about two problems. One is that it is difficult for apples with high heterozygosity to obtain selfed offspring that are

Method used

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  • Method for breaking self-incompatibility of apple to obtain filial generation
  • Method for breaking self-incompatibility of apple to obtain filial generation
  • Method for breaking self-incompatibility of apple to obtain filial generation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the acquisition of self-pollination of each age flower of 'Fuji' apple and the progeny

[0026] 1. Using the 'Fuji' apple as the material, conduct an early pollination test. First, take 200 Fuji flowers in the large balloon stage, peel off the anthers with tweezers and collect them. bottle ready to use.

[0027] 2. Take a small amount of pollen and sow it evenly on the pollen medium (the formula of the medium is 10% sucrose, 0.01% boric acid), seal the greenhouse, and make a mark. After 24 hours in an incubator at 15-25°C, microscopic examination was performed, and three fields of view were observed for each piece. The number of pollen grains was not less than 100, and the germination rate was calculated. The next test can be carried out under the premise of vigorous pollen vitality.

[0028] 3. With the well-growing and robust 'Fuji' apple tree as the plant to be pollinated, 100 sequences of self-pollination experiments with different flowering ages were...

Embodiment 2

[0030] Embodiment 2, the acquisition of 'Golden Delicious' apple 2 instar flower self-pollination and progeny

[0031] 1. Using the 'Golden Delicious' apple as the material, conduct an early pollination test. First, take 200 'Golden Delicious' flowers in the large balloon stage, and use tweezers to peel off the anthers and collect them. After removing the sundries, place them in a cool and dry place to loosen the powder. Bottled for later use.

[0032] 2. Take a small amount of pollen and sow it evenly on the pollen medium (the formula of the medium is 10% sucrose, 0.01% boric acid), seal the greenhouse, and make a mark. After 24 hours in an incubator at 15-25°C, microscopic examination was performed, and three fields of view were observed for each piece. The number of pollen grains was not less than 100, and the germination rate was calculated. The next test can be carried out under the premise of vigorous pollen vitality.

[0033] 3. With the well-growing and robust 'Golden...

Embodiment 3

[0035] Embodiment 3, the acquisition of 'Yue Shuai' apple 3-year-old flower self-pollination and progeny

[0036] 1. Using 'Yueshuai' apples as materials, an early pollination test was carried out. First, 200 flowers of 'Yueshuai' in the large balloon stage were taken, and the anthers were peeled off and collected with tweezers. After the water is cracked, it is bottled for later use.

[0037] 2. Take a small amount of pollen and sow it evenly on the pollen medium (the formula of the medium is 10% sucrose, 0.01% boric acid), seal the greenhouse, and make a mark. After 24 hours in an incubator at 15-25°C, microscopic examination was performed, and three fields of view were observed for each piece. The number of pollen grains was not less than 100, and the germination rate was calculated. The next test can be carried out under the premise of vigorous pollen vitality.

[0038] 3. With the well-growing and well-developed 'Yue Shuai' apple tree as the plant to be pollinated, 100 s...

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Abstract

The invention relates to a method for breaking self-incompatibility of an apple to obtain a filial generation. The method comprises the following steps of: taking apple trees with good growth vigor and strong development as female parent plants, classifying flower buds in different open states, wherein flowers with buds expanding but being covered by sepals are defined as first age class flowers,flowers with the buds protruding out of the sepals are defined as second age class flowers, flowers with the buds breaking through the sepals but being not unfolded are defined as third age class flowers, flowers with the buds opening are defined as fourth age class flowers; selecting the second age class flowers and the third age class flowers to perform self-pollination. The invention provides aclassification method of flower age classes before apple flowers blooming overcomes the reproductive isolation caused by self-incompatibility to a certain extent and successfully obtains the filial generation by performing self-pollination on styles of different flower age classes before blooming. The method can be used for simply and conveniently obtaining an inbred filial generation of the apple which is difficult to obtain by a conventional pollination method; and an effective mean is provided for obtaining genetic materials of allele homozygosity in the apple breeding process.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for overcoming self-incompatibility in the pollination process of apples. Background technique [0002] Since apples were introduced into my country at the beginning of last century, after more than 100 years of development, they have become the fruit with the highest planting area and yield in my country. During the development of the apple industry, the collection of germplasm resources and the selection of new varieties have always been important. It is one of the most effective ways to maintain the vitality of the industry. In the scientific research and production practice related to apple breeding, apple self-incompatibility often brings many difficulties to the development of related work. The self-incompatibility reaction of apple mainly occurs in the style, which is controlled by the multiple allelic locus S-locus. The S locus has multiple different haplotypes,...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 李天忠李洋姜峰吴传宝张懿刘春生于杰
Owner CHINA AGRI UNIV
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