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Method for cultivating new germplasm of epinephelus with stress-resistant hybrid superiority

A breeding method and technology of grouper, which is applied in the field of new germplasm cultivation of grouper with hybrid superiority in growth and stress resistance, can solve the problem of low survival rate of seedlings, lack of fast-growing grouper, high-quality seedlings with strong stress resistance, etc. to improve the survival rate, achieve long-term supply, and avoid vertical transmission

Pending Publication Date: 2020-12-22
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a new germplasm breeding method for growth-resistant and stress-resistant hybrid grouper, aiming to solve the problems of geographical distribution, breeding time and differences in the natural environment of blue-bodied large grouper and brown-spotted grouper. Reproductive isolation problems in terms of breeding temperature, lack of fast-growing and strong stress-resistant seedlings in grouper culture, and low survival rate of seedlings in culture

Method used

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  • Method for cultivating new germplasm of epinephelus with stress-resistant hybrid superiority
  • Method for cultivating new germplasm of epinephelus with stress-resistant hybrid superiority
  • Method for cultivating new germplasm of epinephelus with stress-resistant hybrid superiority

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Screening and breeding of broodstock populations

[0064] 1) In 2014, more than 100 blue-bodied grouper broodstock were collected and screened from Sanya. The screening traits were fast growth in the same group, no disease symptoms, and no deformity traits. The broodstock was over 5 years old, and the screening intensity was 10%; transported to the north under factory conditions, cultured and cultivated; the body weight of the screened blue-bodied grouper broodstock was 23.56±5.53Kg, and the total length was 98-124cm; and the phenotypic traits of the breeding population were measured , as shown in Table 1, select the screening strength according to Table 1.

[0065] 2) Screen and collect more than 1,000 brown-spotted grouper broodstock from the backup populations of different breeding companies in Hainan. The screening traits are fast growth in the same population, no disease symptoms, and no deformity traits. The screening intensity is 5%, and the 4-year-old More than...

Embodiment 2

[0074] Cryopreservation of the sperm of the blue-bodied grouper

[0075]Get the mature blue-bodied grouper male fish obtained in Example 1, and lightly press the abdomen to discharge semen, indicating that this blue-body grouper male fish has reached sexual maturity; select the sexually mature blue-body grouper obtained in Embodiment 1 Large grouper male fish, using 5-10mg / m 3 MS-222 anesthesia; place the broodstock on the delivery bed, use absorbent paper to dry the seawater around the genital opening, push the abdomen backward to make the semen flow out from the genital, and use a straw to suck the semen, such as figure 1 As shown, the specific operation is to squeeze from the abdomen to the genital opening with one hand, and suck the semen with a straw with the other hand; inject it into a 2mL cryovial, and dilute it with sperm cryopreservation solution, as shown in figure 2 As shown, the sperm cryopreservation solution is ETS sperm cryopreservation solution, and the dil...

Embodiment 3

[0080] Cross-fertilization of blue-bodied grouper frozen sperm and brown-spotted grouper eggs

[0081] 1) Reproductive regulation of brown-spotted grouper females

[0082] When the water temperature reaches 22-25°C from March to October every year, the spawning period is from the 1st to the 10th day of each month. Using the natural maturation rhythm of the brown-spotted grouper, the brown-spotted grouper is in its body two days before spawning. Inject ovulation-stimulating hormone analog (LHRH-A3) 5-10μg / Kg to improve maturity and egg production rate.

[0083] 2) Hybrid insemination

[0084] From the 1st to the 5th day of each month, select the female brown-spotted grouper whose abdomen has swollen to maturity, place it on the birthing bed, use absorbent paper to dry the moisture around the genital opening, and push the abdomen from front to back, so that the mature eggs can be released from the birthing bed. The hole flows out, and the eggs are collected in a dry plastic ba...

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Abstract

The invention discloses a method for cultivating new germplasm of epinephelus with stress-resistant hybrid superiority. The method comprises the following steps of (1), screening and cultivating parent fish groups, wherein the selection strength of epinephelus fuscoguttatus is 5%, and the selection strength of epinephelus tukula is 10%; 2), parent reproduction regulating, injecting an ovulation-promoting hormone analogue into parent epinephelus fuscoguttatus, and injecting chorionic gonadotropin and an ovulation-promoting hormone analogue into epinephelus tukula; 3), collecting sperms of the epinephelus tukula, diluting the sperms by using sperm cryopreservation liquid, and performing cryopreservation; 4), performing hybrid insemination, collecting ova, unfreezing sperms, and performing mixed fertilization; 5), hatching to a tail bud stage; and 6), carrying out fry cultivation to obtain fish fries. According to the method, distant hybridization breeding of epinephelus tukula and epinephelus fuscoguttatus is realized, reproductive isolation in the aspects of geographical distribution, propagation time, propagation temperature and the like in the natural environment is overcome, anda new hybrid germplasm with the advantages of fast growth, low oxygen resistance and low temperature resistance is cultivated.

Description

technical field [0001] The invention belongs to the technical field of hybrid breeding, in particular to a method for cultivating new germplasm of growth-resistant and stress-resistant hybrid dominant grouper. Background technique [0002] Brown-spotted grouper, Epinephelus fuscoguttatus, also known as tiger spot, is widely distributed in the Indo-Pacific region, extending eastward to Central Pacific Samoa and the Phoenix Islands, from southern Japan in the north to Australia in the south; my country is mainly distributed in the Taiwan Strait and the South China Sea and the eastern coast of Taiwan. The body of the brown-spotted grouper is light yellow-brown, with five vertical series of dark brown spots forming irregular stripes; the body is distributed with small brown spots, and it is one of the main species of grouper cultured. The brown-spotted grouper grows slowly, and the growth weight of the cultured seed was only 300-493g; however, the meat is delicious, the reproduc...

Claims

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Application Information

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IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 田永胜王林娜李振通李子奇陈帅黎琳琳段鹏飞王心怡
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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