Haploid induction line genetic purity preservation method based on reproductive isolation characters

A haploid induction line and haploid induction technology, applied in the field of plant genetics and breeding, can solve the problems of long cycle and large human, financial and material resources, and achieve the goal of reducing the probability of field contamination, reducing work tasks and improving breeding efficiency. Effect

Active Publication Date: 2021-10-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional method adopts hybridization and multi-generation backcrossing to obtain pure lines, which takes a long period and consumes a lot of manpower, financial and material resources

Method used

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  • Haploid induction line genetic purity preservation method based on reproductive isolation characters
  • Haploid induction line genetic purity preservation method based on reproductive isolation characters
  • Haploid induction line genetic purity preservation method based on reproductive isolation characters

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the construction of haploid induction line with unidirectional reproductive isolation performance

[0043] 1. Breeding process of haploid induction line with unidirectional reproductive isolation performance

[0044] In order to aggregate the unidirectional reproductive isolation traits and haploid-induced traits, the gene for reproductive isolation traits was introduced into the haploid induced line, and finally the haploid induced line of maize was kept pure. First, the CAU5 (molecular marker) was used to detect its content The key induction locus genes ZmPLA1 and ZmDMP are both recessive homozygous) as the female parent, and the popcorn line 401D (the reproductive isolation gene contained in it detected by molecular markers, that is, the unidirectional hybrid incompatibility gene Ga1-S is dominant homozygous) is The male parent is crossed to obtain F 1 generation of hybrid seeds; planting F 1 F 1 Generation hybrid maize plants, with CAU5 as the recurre...

Embodiment 2

[0077] Example 2. Character evaluation of unidirectional incompatibility haploid induction line pCAU5

[0078] 1. One-way incompatibility verification

[0079] In May 2020, the target inducible line pCAU5 obtained in Example 1 and a variety of conventional materials (including commercial hybrids ZD958, ND678 and JK968 and conventional self-selected hybrids bred by Chen Shaojiang's research group) were planted in sub-plots at the Shangzhuang Experimental Station of China Agricultural University. Cross-strain C229, Chen04_065, Chen04_125. Among them, ZD958 was purchased from Beijing Denong Seed Industry Co., Ltd., ND678, C229, Chen04_065, Chen04_125 were provided by Chen Shaojiang’s research group of China Agricultural University, and JK968 was purchased from Beijing Shunxin Agriculture Co., Ltd. None of the materials contained Ga1-S gene and R1-nj color marker gene).

[0080] Plot 1: Set up three repeated areas, plant 100 pCAU5 plants in each repeated area, and 50 commercial h...

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Abstract

The invention discloses a haploid induction line genetic purity preservation method based on reproductive isolation characters. According to the method, a reproductive isolation character gene is applied to genetic purity preservation work of the induction line to form a haploid induction line material which only accepts self pollen and has a biological isolation function. The haploid induction line material with the biological isolation function is beneficial to avoiding the influence of external pollen on the induction performance of the induction line in commercial DH breeding application, solves the genetic purity preservation problem in the induction line breeding process and provides a feasible scheme for solving the problem of purity preservation work of a key induction line material in the haploid breeding process, the breeding workload of the induction line can be greatly reduced, and the material purity of the induction line can be ensured; and a new thought is provided for breeding work of pop corn and part of sweet-waxy corn materials, and popularization and application of a haploid breeding technology are promoted.

Description

technical field [0001] The invention belongs to the field of plant genetics and breeding, and in particular relates to a method for genetically purifying haploid induced lines based on reproductive isolation traits. Background technique [0002] Maize is a typical heterosis and cross-pollination crop, and the core of its breeding is to obtain excellent inbred lines. The traditional method adopts hybridization and multi-generation backcrossing to obtain pure lines, which takes a long period and consumes a lot of manpower, financial and material resources. Haploid breeding technology can obtain pure lines within 1-2 years through induction, identification, doubling and other processes, which greatly shortens the breeding cycle and improves breeding efficiency. It has become one of the mainstream technologies for maize breeding. As one of the key materials in the technical process of haploid breeding, the purity of the induced line is the most important. The key induction gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02C12Q1/6895C12Q1/6858C12N15/11
CPCA01H1/021C12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2600/172C12Q2531/113C12Q2565/125
Inventor 陈绍江郭树威焦炎炎刘晨旭陈明钟裕陈琛祁晓龙程德荷李金龙
Owner CHINA AGRI UNIV
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