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Rice breeding method capable of preventing pollen drifting

A technology of pollen drift and rice seeds, applied in the direction of botany equipment and methods, applications, plant genetic improvement, etc.

Inactive Publication Date: 2009-03-11
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the escape of exogenous genes and the possible environmental risks are the core issues of transgenic safety. The genetic characteristics of rice and its cultivation and geographical distribution indicate that transgenic rice has a major potential risk of gene flow between cultivated rice and wild relatives.

Method used

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  • Rice breeding method capable of preventing pollen drifting

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, a kind of rice breeding method of preventing pollen drift, is used for the seed production of three-line japonica hybrid rice and prevents miscellaneous, specific content is as follows:

[0018] 1. Using hybridization, test crossing and backcrossing methods, the hybrid disadvantageous gene in Peruvian rice jamaica is introduced into the restorer line, sterile line and maintainer line of the three-line japonica hybrid rice, so that the genotype of the reproductive isolation site Replaced from aaBB type to AAbb type. Follow the steps in order:

[0019] ①. After crossing the Peruvian rice variety Jamaica with the restorer line Cbao and the maintainer line Wuyunjing 7, a dominant and weak F 1 . Due to the taller plants of Jamaica, we 2 Select plants with good leaf shape from the population and continue to backcross with Cbao and Wuyunjing 7. In the corresponding backcross F 1 Lieutenant generals have three phenotypes: one is all vulnerable; the other is ha...

Embodiment 2

[0030] Embodiment 2, a kind of rice breeding method of preventing pollen drift, is used for the seed production of two-line japonica hybrid rice and prevents miscellaneous, specific content is as follows:

[0031] 1. Using methods of hybridization, test crossing, and backcrossing, introduce the hybrid disadvantageous gene in the Peruvian rice jamaica into the two-line japonica-type sterile line and the restorer line, so that the two-line japonica-type sterile line and the restorer line are at the reproductive isolation position The genotype of the point is replaced by AABB type from aaBB type. Follow the steps in order:

[0032] ①. A dominantly weak F 1 , at F 2 Select sterile plants from the population for backcrossing with 7001S; select a series of lines with good leaf shape and stable sterility under long-day high temperature from the segregating offspring (you can follow step ① for continuous backcrossing, and select lines similar to the parents. strain); the steps of i...

Embodiment 3

[0042] Embodiment 3, a rice breeding method for preventing pollen drift, used to prevent genetically modified rice genes from escaping, the specific contents are as follows:

[0043] 1) Using hybridization, test crossing, and backcrossing methods to introduce the hybrid weak gene in Peruvian rice jamaica into herbicide-resistant transgenic rice, so that the genotype of the herbicide-resistant transgenic rice at the reproductive isolation site is replaced by aaBB type Into AAbb type. Follow the steps in order:

[0044] ①. Crossing the Peruvian rice variety Jamaica with the transgenic rice Jiahe 212 containing the Bar gene to obtain hybrid weak F 1 . Due to the taller plants of Jamaica, we 2 The plants with good leaf shape were selected from the population to continue backcrossing with the transgenic rice containing the Bar gene, and a series of plants with good leaf shape were obtained in the segregated population.

[0045] ②. Spray the obtained strains with 0.25% Basta her...

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Abstract

The invention discloses a method of paddy rice breeding with the function of preventing pollen from flowing. The method comprises the following steps: a paddy rice reproductive isolation gene is introduced into the paddy rice so that cross-pollinated hybrid F1 cannot grow normally in a paddy field, thus achieving the purpose of preventing the pollen from flowing and ensuring purity of the paddy rice seed. The paddy rice reproductive isolation gene can be chosen from any one of the genes which can generate the following characteristics: hybrid weakness, hybrid lethality, hybrid collapse, hybrid sterility and hybrid depression.

Description

technical field [0001] The invention belongs to the field of production methods of rice seeds. Specifically, the present invention relates to a new breeding method that uses the reproductive isolation mechanism of rice to avoid manual removal of impurities, prevent pollen drift, ensure the purity of rice seeds and prevent the escape of exogenous transgenes. Background technique [0002] The successful use of rice heterosis in my country in the 1970s increased the rice yield by about 20% on the basis of dwarf breeding, and achieved huge social and economic benefits. At present, the seed production methods of hybrid rice mainly include two-line and three-line methods, and the purity of hybrid rice seeds directly determines whether heterosis can be popularized and applied in production; Seed production isolation work will spend a lot of manpower, material and financial resources. [0003] Plant transgenic technology is one of the most remarkable achievements in the field of l...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 钱前胡江郭龙彪曾大力张光恒董国军胡兴明颜美仙高振宇
Owner CHINA NAT RICE RES INST
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