Macrobracium rosenbergii spermatozoon gelatinases, encoding gene and application thereof

A technology of Macrobrachium rosenbergii and coding genes, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc., and can solve the problems that there are no detailed reports on proteins or genes
CN101368178AInactive Publication Date: 2009-02-18ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2009-02-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to protease-(M.rosenbergii sperm gelatinase-like protease, SGP) with the hydrolyzed gelatin function, which is purified from the sperm crude extract of giant freshwater prawn (M.rosenbergii), and a coded gene and an application thereof. The studies of enzyme related with fertilization on sperms of different species are helpful to understanding the mechanism of crustacean fertilization and the mechanism of the production of reproductive isolation between different species. Based on the theories and the techniques, the relevant researchers can establish a good hybrid system, thus obtaining high yield and fast growth hybrid species.
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Description

(1) Technical field

[0001] The present invention relates to macrobrachium rosenbergii sperm gelatinase-like protease (sperm gelatinase-like protease, SGP) and its coding gene and application. (2) Background technology

[0002] During fertilization, the combination of sperm and egg has the characteristics of specificity among species. As one of the regulators of plasma membrane fusion, proteins on sperm play an important role in this process. In mammals, such as mice, a sperm protein called Izumo is a key factor in the fusion of sperm and egg plasma membranes. Another sperm surface protein, cyritestin, plays an important role in the specific adhesion of sperm to eggs. Proteins (proteases) on sperm also play an important role in the fertilization process in invertebrates. Like abalone, mussels and several snails, the sperm protein lysin is considered to be the main factor for sperm to penetrate the egg membrane. However, in shrimps belonging to arthropods, there are no det...

Claims

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