Cowpea variety molecular identification method based on genome RAPD analysis

A technique for molecular identification and analysis of varieties, applied in the field of molecular identification of cowpea varieties based on genome RAPD analysis, can solve problems such as undeveloped, and achieve the effects of short time, information diversity, and multiple polymorphisms

Inactive Publication Date: 2005-11-30
JIANGHAN UNIVERSITY
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Problems solved by technology

[0005] The research on molecular markers of genetic polymorphisms of lo

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  • Cowpea variety molecular identification method based on genome RAPD analysis
  • Cowpea variety molecular identification method based on genome RAPD analysis
  • Cowpea variety molecular identification method based on genome RAPD analysis

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[0052] The present invention is further described below, and the present invention comprises the following steps:

[0053] (1), extract the DNA of existing 40 long cowpea varieties leaf genome cowpea variety molecules;

[0054] (2), carry out polymerase chain reaction respectively with the genomic DNA of the extracted cowpea variety to obtain the random amplified polymorphic DNA product;

[0055] (3) Use 1.0% agarose to separate the randomly amplified polymorphic DNA products by electrophoresis, add EB directly to the gel, and the total loading volume is 8 μl, of which 4 μl of amplified products are added with 40% sucrose solution 2μl and 2μl of 0.1% bromophenol blue, electrophoresis at constant voltage at 80V for 1.5h;

[0056] (4) After electrophoresis, take a photo on an ultraviolet light detector to obtain a standard atlas of randomly amplified polymorphic DNA;

[0057] (5) Select a clear random amplified polymorphic DNA pattern to record the bands using the "0-1" system...

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Abstract

The invention discloses a method for molecular inspection of cowpea variety based on analysis of genome RAPD. Extracting DNA of the available genome cowpea varieties, and generating separately polyase chain reaction to prepare the random augmentative polymorphic DNA product, then processing electrophoretic separation to obtain random augmentative polymorphic DNA standard atlas; analyzing the inspection-required cowpea variety following said steps to get the random augmentative polymorphic DNA atlas, comparing and deciding it with standard atlas, the inspection result is obtained. With the invention, it can definitely decide the different varieties of cowpea, and realize precise and rapid inspection of variety truth and variety purity, providing reliable technique of variety inspection, market control of seed, variety protection, quality control of pre-sowing seed, stock breeding and so on.

Description

technical field [0001] The invention relates to a method for plant genetic markers and variety molecular identification, in particular to a method for molecular identification of cowpea varieties based on genome RAPD analysis. Background technique [0002] Cowpea is an annual herb. Originated in tropical Africa, widely distributed in tropical and subtropical regions. There are three cultivated subspecies of common cowpea (Vigna unguiculata ssp. unguiculata), long pod cowpea (Vigna unguiculata ssp. sesquipedalis) and short pod cowpea (Vigna unguiculata ssp. cylindrica). China is the center of secondary origin of long cowpea (Vigna unguiculata cv-gr.sesquipedalis E.Westphal), with a long history of cultivation and rich variety resources. In recent years, although great achievements have been made in genetic breeding, it still cannot meet the urgent demands of the market for new varieties. New varieties of cowpea are bred through genetic improvement, especially those with var...

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 陈禅友胡志辉赵新春
Owner JIANGHAN UNIVERSITY
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