321results about How to "Simplify the sampling process" patented technology

The invention relates to a water quality sampling robot for full-automatic surface water, which comprises a hull, a driving device, a control device and a sampling device, wherein the control device comprises a navigation device, a central processing unit and a communication module; the sampling device comprises a weather and water quality monitoring sensor, an underwater sensor and a sampling pipe retractable device; the navigation device comprises a GPS satellite positioning sensor, an electronic compass and an acceleration sensor; the navigation device is electrically connected with the central processing unit and the communication module; the communication module is connected with a base station through a wireless signal; the hull is provided with a radar, a laser ranging sensor obstacle avoiding device, a fixing agent dripping device and an insulation box; and the control device is electrically connected with the driving device and the sampling device to realize automatic sampling and automatic navigation on the hull. The water quality sampling robot has the advantages of small volume, light weight, adoption of electric drive and no pollution emission; and compared with large-scale sampling vessels, the water quality sampling robot not only saves energy consumption and labor power, but also reduces cost and use threshold.

The invention discloses a roof regeneration medium-length hole ore blast backfill mining method, which comprises the following steps of: dividing an ore body into middle sections, reserving continuous ore pillars in the middle along the advancing direction, dividing the ore body into a panel through panel barriers along the tilt direction, and laying out a plurality of stopes in the panel along the advancing direction; arranging a scraper main access road on a base plate of the continuous ore pillars along the advancing direction, using the scraper main access road as a communicating channel to communicate with various topes, arranging a scraper branch access road on a base plate of the panel barriers along the tilt direction, and using the scraper branch access road as a channel to communicate with various middle sections; and stoping an upper layer and a lower layer integrally divided in the panel, namely stoping the ore body in the upper layer, performing waste stone cemented filling to obtain an artificial roof, performing high-efficiency medium-length hole ore blast on the ore body in the lower layer, removing ore by using a scraper, making the ore pass through a stope chute to descend to a panel haulage roadway, and loading and conveying the ore on an underground electric locomotive. By the method, a safe stoping environment is recreated, a high-efficiency mining process is implemented, a preliminary mining process is simple, operation interference between the upper and lower layers is small, the loss and dilution are effectively controlled, and the production cost is greatly reduced.

A dynamic non-rigid body three-dimensional digital method fusing RGB-D camera and color photometric stereo method employs an RGB tricolor light source to irradiate an object under test, and employs a depth camera and a color camera with internal parameters calibrated to photograph the object to acquire a depth image and a color image of the object; the method then estimates light source direction and reflectivity in conjunction with depth and color information; a cost function fusing a depth item, a normal item and a smooth constraint item is constructed by variational method, and an improved-quality surface three-dimensional digital model of the object under test is solved by least squares optimization. By using the method, it is possible to effectively solve the problem that an existing dynamic non-rigid body three-dimensional digital method has matching difficulty in non-rigid body objects in different inter-frame images, has great geometric error or has poor smoothness and poor detail fineness of reconstruction results due to noise impact; the method has the advantages of high efficiency, high precision and low cost, and is applicable to a three-dimensional digital task for a dynamic non-rigid object to provide efficient and convenient object three-dimensional reconstruction.

The invention discloses a soil wind erosion measuring method. The method comprises the following steps: determining a circular measuring area and selecting a plurality of measuring points on circumference; respectively distributing one sand collection device of which opening faces the circle center and one sand collecting device of which opening is far away from the circle center on both sides of the measuring points along the circumference direction and respectively collecting wind erosion matters leaving and going into the measuring area; calculating sand transport fluxes Qin and Qout going into and leaving the measuring area, wherein the different value of the Qin and Qout is the single width sand transport rate Q of the measuring area, and the single width sand transport rate Q is substituted into a formula of A=Q*2pir / pir<2> to obtain soil wind erosion amount. According to the soil wind erosion measuring method, the wind direction in the same wind erosion incident does not need to keep stable, change of sand transport amount along with length does not need to be calculated, non-erosion boundary does not need to be defined, the applicability is increased, the calculating method is simplified, and the measuring accuracy is improved simultaneously. The invention also discloses a soil wind erosion measuring device. The soil wind erosion measuring device is high in accuracy.

The invention relates to a method for rapidly representing sludge dewatering performance. The method comprises the following steps of: collecting 100ml of raw sludge or concentrated sludge; filtering with a filter membrane of 0.45mu m to obtain 10mL of sludge supernatant; measuring soluble organic carbon (DOC) of the supernatant; diluting the supernatant with distilled water until the DOC is less than 10mg / L; collecting a three-dimensional fluorescence spectrum (EEM) of the diluted supernatant; substituting the collected EEM spectrum into a database to analyze a parallel factor (PARAFAC) by using a DOMF1uor tool kit of Mat1ab 7.0 data processing software to determine a score of a component with excitation and transmission wavelength of (220,280) / 350nm; and multiplying the score by a diluting multiple of a sample and substituting the product into a sludge dewatering performance judgment relational formula to represent the sludge dewatering performance. By the method, the sludge dewatering performance can be rapidly detected without chemical reagent, and the detection can be completed only through filtering, diluting and three-dimensional fluorescence spectrum; and the method can be applied to monitoring the dewatering performance of various types of sludge of sanitary sewage treatment factories, industrial sewage biological treatment factories and the like.

The invention discloses a digital automatic frequency correction method based on real frequency reference signals, which relates to the technical field of frequency synchronization of a transiting side and a receiving side in communication. The method comprises the following steps: sampling a channel of real received signals; under the condition that the real frequency reference signal exists in the received signals, calculating the frequency error function of the local frequency source signal and the received frequency reference signal; correcting the frequency of the local frequency source according to the frequency error function, thereby achieving the frequency synchronization between the transiting side and the receiving side. Compared with the prior frequency correction method based on complex frequency reference signals, the digital automatic frequency correction method doesn't need to acquire the mutually-orthogonal complex frequency reference signals of the channel I and the channel Q; overcomes the problem of imbalance compensation of the channel I and the channel Q; and therefore, greatly reduces the complexity of system implementation on the premise of ensuring the frequency correction accuracy.

The invention relates to a detection method for the quality of a lithium battery pole piece. The method comprises the steps of picking up points uniformly from a pole piece coating region, testing the resistance between a pole lug region and the points picked from the coating region, and then determining whether the coating is uniform by determining whether the resistance is uniformly distributed. The method can reflect the coating quality of the pole piece more objectively. The detection method provided by the invention facilitates the detection of the quality of the battery pole piece, and can provide feedback and guidance to process adjustment.

The invention relates to a linear frequency modulation signal sparse sampling and reconstruction method based on a fractional Fourier transform domain. The linear frequency modulation signal sparse sampling and reconstruction method comprises the steps of: carrying out parameter initialization, so that information of an analog chirp signal is reserved at the same time at a low frequency band of apseudo-random code; filtering high-fraction Fourier transform spectrum components of the analog chirp signal and a pseudo-random code signal, and outputting a filtered signal; sampling the output filtered signal by a low-speed analog-to-digital converter to obtain a sampling value; calculating a sparse representation coefficient of the analog chirp signal in a fractional Fourier transform domain through adopting a matching pursuit algorithm; and acquiring an original analog chirp signal at the reconstruction position by means of fractional Fourier transform domain sparse representation. According to the linear frequency modulation signal sparse sampling and reconstruction method, the sparse property of the chirp signal in the fractional Fourier transform domain is utilized, so that the problem that compressed sampling cannot be completed in the frequency domain can be solved.

The invention provides a reverse circulation drill for geological prospecting, and a power head of the reverse circulation drill. The reverse circulation drill for geological prospecting is high in drilling efficiency. The power head comprises a rotating joint, a rotary box and a gas distributor, wherein the rotating joint, the rotary box and the gas distributor respectively comprise a rotating shaft, a main shaft and a mandrel which are hollow shafts arranged in a coaxial through way and are sequentially connected in a sealing way; the outer end of the rotating shaft is provided with a slag discharge hole; the main shaft is connected with a driving motor; the mandrel is internally provided with an inner tube in a coaxial way; the inner end of the inner tube is connected with the inner wall of the mandrel in a sealing way; the inner end of the inner tube is communicated with a hollow part of the main shaft is through; the outer diameter of the inner tube is smaller than the inner diameter of the mandrel; and at least one air inlet is arranged along the circumferential direction of the inner end of the mandrel, and compressed air enters the mandrel from through the air inlet. All the parts of the power head are independent of one another, so that the power head is convenient to assemble and disassemble; therefore, the sampling process is simplified, the quality of a sample is guaranteed, and the exploration efficiency is improved.

The invention discloses a soil detection device with a bottom plate auxiliary structure. The soil detection device comprises a mounting vehicle plate, pushing handlebars are fixedly connected to thetwo sides of one end of the mounting vehicle plate correspondingly, and a storage battery is fixedly connected to one end of the top of the mounting vehicle plate. A sweeping motor is fixedly connected to the bottom end of the mounting vehicle plate, an output shaft of the sweeping motor is fixedly connected with a shell of a bottom electric telescopic rod, and an output shaft of the bottom electric telescopic rod is fixedly connected with a sweeping circular plate. Brush wires are fixedly connected to the bottom end of the sweeping circular plate, a powder barrel is fixedly connected to the edge of the sweeping circular plate, and the bottom end of the powder barrel communicates with a powder outlet pipe penetrating through the sweeping circular plate. An electromagnetic valve is arrangedon the powder outlet pipe, the sweeping circular plate is surrounded by a shell fixedly connected to the mounting vehicle plate, and the lower edge of the shell is higher than the lower edges of therolling wheels. At least two mounting holes are formed on the side wall of the shell, auxiliary electric telescopic rods are fixedly connected to the upper walls of the mounting holes, and the outputrods of the auxiliary electric telescopic rods are fixedly connected with the auxiliary suction cups.

The invention relates to a detection method and a detection system for rock sample three-direction permeability. The detection method comprises that: a rock sample requiring detection is labeled according to axial directions of a three-dimensional rectangular coordinate system, and then is sequentially placed in a detection system along the labeling directions, sealing pressurization is performed, and then the permeabilities along different labeling directions are sequentially measured. The detection system comprises a clamping unit, a gas injection unit, a detection unit and a control unit, wherein the gas injection unit is connected with the clamping unit, the detection unit is arranged outside the clamping unit, the control unit is connected with the detection unit, the clamping unit comprises a clamper 12, a confining pressure sleeve 1, a transition sleeve 4 and a rock sample requiring detection 3 sequentially form a sleeved connection in the clamper 12, and one end of the rock sample requiring detection is connected with the gas injection unit. According to the present invention, drilling of the core column on the core is not required, and the cutting grinding manner is adopted to sample so as to improve the sampling success rate. In addition, with the detection method and the detection system, the three-direction permeability test can be performed on the one rock sample so as to improve the utilization rate of the rock sample and enhance the comparability of the test result.

The present invention discloses a diagnosis antigen for Toxoplasma gondii infection and a preparation method and applications thereof, belonging to the technical field of biological veterinary drugs. An antigen GRA8 suitable for diagnosis for Toxoplasma gondii infection is screened out by using methods combining Western blot, two-dimensional electrophoresis, and MALDI-TOF-TOF tandem mass spectrometry. The antigen can be used for a variety of molecular biology diagnosis and serological diagnosis for the Toxoplasma gondii infection.

The invention discloses a detection method for solvent gasoline in air in a working place, and relates to the technical field of solvent gasoline detection. The method comprises the following steps of collecting an air sample by using an activated carbon tube, collecting a blank air sample by using a blank sample collector, and delivering the samples to a laboratory for storage; preparing a desorption solution; pouring activated carbon in the activated carbon tube into a solvent desorption bottle, adding the desorption solution into the solvent desorption bottle, and taking the upper desorption solution as a detection solution; debugging a gas chromatograph, and setting a temperature raising program, wherein a bonded and cross-linked chromatographic column is adopted as a gas chromatographic column; preparing a standard solution by adopting carbon disulfide; injecting the upper desorption solution into the gas chromatograph for detection, wherein the injection volume of the desorption solution is 0.5 or 10 microlitres; and quantitatively analyzing a detection result to obtain the concentration of the solvent gasoline in air. According to the detection method, a process is simple, the result is accurate, the method is wide in application range and short in detection time, and the solvent gasoline in air in the working place can be timely detected.

The invention discloses a digital automatic frequency detection method based on real signals, which relates to the technical field of frequency synchronization of a transiting side and a receiving side in communication. The method comprises the following steps: sampling a channel of real received signals; calculating the detection threshold and the measurement of the frequency reference signal; and determining whether the frequency reference signal exits in the received signals according to the calculated detection threshold and the measurement, wherein the frequency reference signal is utilized to maintain the frequency synchronization between the transiting side and the receiving side during the frequency correction process. Compared with the prior frequency detection method based on complex signals, the digital automatic frequency detection method doesn't need to acquire the mutually-orthogonal complex received signals of the channel I and the channel Q; overcomes the problem of imbalance compensation of the channel I and the channel Q; and greatly reduces the complexity of system implementation on the premise of ensuring the frequency detection performance.

The invention provides a frequency converter three-resistance current sampling method based on current prediction. In each PWM (Pulse-Width Modulation) period, current closed loop PID (Proportion Integration Differentiation) control and SVGEN modulation are performed, and PWM comparison values T<1>, T<2> and T<3> of three phases A, B and C are obtained; the sizes of the T<1>, the T<2> and the T<3> and the shortest time T<min> required by complete sampling are compared in real time; and different current obtaining modes are used according to different size relationships. The method provided by the invention solves the problem that the three-phase current cannot be accurately sampled due to zero-voltage vector duration insufficiency at a high modulation ratio of the conventional three-resistance sampling technology of a three-phase frequency converter. The wide region current closed loop control under the three-resistance sampling can be realized; the modulation ratio is improved; the motor load capability is improved; the cost is low; and the sampling is simple and reliable.

The invention provides a water treatment photocatalytic reaction device. The device comprises a light shielding shell, a xenon lamp light source, a light filtering baffle plate, a fixed plate, reaction beakers and heat dissipation ventilation fans, a magnetic stirrer, a cooling water circulating pump and a red metal and rubber pipeline. The invention is mainly used for photocatalyst performance evaluation and kinetics study of photocatalytic degradation of organic pollutants. The invention provides a water treatment photocatalytic reaction device able to carry out batch photocatalytic reaction study, and the device has the advantages of simple technological structure, low energy consumption and high efficiency.

The invention discloses an unmanned aerial vehicle for water-detecting sampling. An unmanned aerial vehicle body is arranged on an installation frame through an installation plate, and threaded holeswith the same bore diameter are separately formed in the installation plate and the installation frame in a corresponding mode, and the installation plate is fixedly arranged on the installation framethrough a fastening bolt in a threaded connection mode. A through hole is formed in a rotary table and a rubber plug is arranged in the through hole, a test tube placing slot with the same bore diameter as the through hole of the first rotary table is formed in a second rotary table, a gravity sensor is arranged at the bottom of the test tube placing slot, and a cushion pad is arranged between the gravity sensor and a test tube. A sliding slot is formed in the installation frame, a sliding block is connected into the sliding slot of the installation frame in a sliding mode, a rack is welded on the upper surface of the sliding block, the rack and a gear are connected on the installation frame in an engaged mode, and a transverse plate is arranged on the lower surface of the sliding block.

The invention provides a gain compression and interception waveform sampling method for a transient short optical pulse semiconductor optical amplifier. , the sequence pulse amplitudes are arranged according to a fixed attenuation ratio. Then, according to the pulse sequence timing determined by the length of the passive optical fiber loop, the pulse is intercepted by the semiconductor optical amplifier. The intercepted pulse waveform is sent to a PIN photocell with a bandwidth of 100 megabytes, and then the data acquisition card is used to measure the output pulse amplitude of the PIN photocell to obtain the energy envelope of the intercepted pulse, and then combine the passive fiber ring pulse to copy. The original transient short optical pulse waveform is reversed by the replica amplitude variation law of the generator. The method provided by the invention adopts a simple system structure, low equipment requirements, easy debugging and integration, and can be used as an effective means for measuring transient short light pulses.

The invention belongs to the field of genomics and bioinformatics, and relates to a urinary sediment genome DNA classification method and device and application. Specifically, the DNA classification method comprises the following steps: calculating an MHL value of a DNA methylation haplotype region of a target sample and / or copy number variation data of DNA; calculating the similarity between theMHL value of the DNA methylation haplotype region of the target sample DNA and the MHL value of the DNA methylation haplotype region of each classification label, and / or the similarity between the copy number variation data of the target sample DNA and the DNA copy number variation data of each classification label; and according to the similarity, determining the classification to which the target sample DNA belongs by using a classifier model. The invention provides a novel detection means for urogenital system tumors, and the detection means has good specificity and sensitivity.

The invention discloses a power supply with wide output voltage range, which comprises more than one group of branch circuits, wherein the more than one group of branch circuits comprise a first branch circuit and a second branch circuit, the first branch circuit and the second branch circuit are the same in structure, a first change-over switch is arranged on the first branch circuit, a second change-over switch is arranged on the second branch circuit. When high voltage needs to be output, the output change-over switch is controlled, the second pin and the third pin of the first change-overswitch and the second pin of the second change-over switch are connected, and the output sides of the first branch circuit and the second branch circuit work in a series connection mode. When low voltage needs to be output, the first pin and the third pin of the first change-over switch and the second change-over switch are controlled to be connected, and the positive electrode end and the negative electrode end of the first branch circuit and the second branch circuit are connected with a capacitor C. The invention has the advantages that: the current of each branch of the circuit is simple to sample, isolation is not needed, the cost is low, and the precision is high; the change-over switch has no large peak current and cannot be damaged by large current; through staggered driving, the ripple current of the inductor and the capacitor can be reduced when the inductor and the capacitor are connected in series and in parallel.

The present invention discloses a water sampling device for water conservancy and reservoirs. The device comprises a bracket, and a guiding sleeve is arranged on top of the bracket, a guiding rod is slidably connected within the guiding sleeve, one end of the guiding rod is provided with a driving mechanism, and the other end of the guiding rod is connected with a rack, and a cross rod is connected to one side of top of the bracket, and a bearing seat is embedded at one end of the cross rod, a first rotating shaft is connected within the bearing seat, upper end of the first rotating shaft is sleeved with a gear, and the gear is meshed with the rack, a fixing plate is connected to lower end of the first rotating shaft, a cylinder is respectively arranged at both ends of bottom of the fixingplate, a piston rod of the cylinder is connected with a lifting plate, a first motor is arranged at top of center of the lifting plate, an output shaft of the first motor passes through the lifting plate and is connected with a second rotating shaft, a bottom plate of the second rotating shaft is connected with a take-up drum, and two ends of bottom of the lifting plate is respectively provided with a first water sampling mechanism and a second water sampling mechanism. The device is convenient and quick to use, makes sampling more dispersed, is more universal, has sampling process that is simple and easy to operate, and improves work efficiency.

The present invention proposes a method for controlling a roasting process of coffee beans comprises sampling a first batch of coffee beans and a second batch of coffee beans from said coffee beans during roasting(S12; S42); detecting a surface colour of the first batch of coffee beans(S14; S44); grinding the second batch of coffee beans and detecting a powder colour of the second batch of coffee beans after grinding(S16; S46); and controlling the roasting process at least partially based on the detected surface colour of the first batch of coffee beans and the detected powder colour of the second batch of coffee beans(S18; S48). The present invention also provides apparatus using the above described method.

The invention discloses a content-based user personalized product matching recommendation method. The problem that in the prior art, a recommendation algorithm still needs to be improved is solved. The method comprises the following steps: step 1, a random batch sampling method based on a user; step 2, a content-based user product matching method: establishing a network; according to a batch inputuser-based random batch sampling method, obtaining an ordered set of a user id, a historical record product id list, a target product id and label set, and training, adjusting and evaluating a network model on the training set, the verification set and the test set respectively; and inputting a specific user and historical records thereof, predicting scores of the specific user for all unwatchedmovies by using a content-based user product matching network, sorting the scores, and finally outputting a top-N recommendation result. According to the method, the lightweight neural network is adopted, so that the training time and training equipment requirements are greatly reduced, the sampling process is easy, the model input is more random, and the generalization ability of the prediction result is stronger.

The invention discloses a culture medium for efficiently culturing umbilical cord blood CIK cells in vitro and a culture method thereof, which belong to the technical field of biology. The culture medium is prepared from the following components: an AlyS505N-0 serum-free basal culture medium, a cytokine composition A and a natural traditional Chinese medicine extract. The culture method comprisesthe following steps: collecting and separating umbilical cord blood mononuclear cells; resuspending cells, adding IFN-gamma for activation, incubating in a 6% CO2 incubator at 37 DEG C, adding the cytokine composition A for stimulation on the next day, supplementing the culture medium containing a traditional Chinese medicine extract B after 3-5 days, supplementing the culture medium containing the traditional Chinese medicine extract composition B every 2-3 days during culture, and co-culturing for 15-17 days to obtain a large number of CIK cells. According to the culture medium and the culture method thereof, a large number of CIK cells can be proliferated, and CD3+CD56+ double positive cells in the cells can reach 70% or above.

The invention discloses an unmanned plane for detecting hazards in a work place. The unmanned plane comprises an unmanned plane boy, a sampling module, and a collecting module, wherein the sampling module and the collecting module are arranged on the unmanned plane body. The collecting module comprises a ratchet wheel rack, collection pipes, and a positioning gear, wherein the collection pipes and the positioning gear are arranged on the ratchet wheel rack. The left side of the ratchet wheel rack is provided with a negative pressure pump. The right side of the ratchet wheel rack is connected to the sampling module through a breather pipe. Through the design that the ratchet wheel rack of the collecting module drives the switching of the collection pipes, automatic and continuous sampling of hazard factors in a work place is realized. The time and region limits of sampling are broken through. The unmanned plane can carry out sampling in a hazardous environment and has the advantages of simple structure, lightness, flexibility, and convenient operation. The unmanned plane can carry out multi-point sampling of multiple hazard factors.

The invention provides a distributed compression sensing-based hyperspectral image compression method. The method comprises the steps of based on correlation of each band spectrum and information entropy of each band spectrum in a hyperspectral image, dividing all bands into a low correlation band group and multiple high correlation band groups, wherein each high correlation band group comprises a reference band and multiple non-reference bands; determining a region of interest and a background region in the reference band; for each non-reference band, performing differential processing on the region of interest and background region in the reference band, so as to acquire corresponding residual images; sequentially compressing residual images and low correlation band images of the region of interest and background region of each high correlation band group and each non-reference band; and sending code streams of all compression codes. According to the method, different distributed compression processing is performed on different bands and different regions, so that important information of the hyperspectral image is fully protected, and the compression rate of the hyperspectral image is improved.

The present invention discloses a variable fractional sampling rate selection method. The method comprises: obtaining the range of values of the sampling rate and the sampling rate conversion multiplewhen antialiasing, wherein the range of values is related to the repetition frequency of the output waveform; and according to the ratio of the maximum sampling rate of a DDWS waveform generator to the repetition frequency of the output waveform of the DDWS waveform generator, the relationship between the cutoff frequency of the filter in the DDWS waveform generator and the maximum sampling rateof the DDWS waveform generator, and the relationship between the highest frequency of the output waveform of the DDWS waveform generator and the maximum sampling rate of the DDWS waveform generator, obtaining the specific value of the sampling rate conversion multiple and obtaining the sampling rate. The sample rate points can be reduced as much as possible while avoiding spectrum aliasing, so that the DDWS waveform generator hardware workload and the required storage depth are reduced. The sampling rate in the present invention can be changed with the frequency of the output waveform, the relationship between the sampling rate and the output waveform is closer and more flexible, and the hardware workload of the instrument is reduced.

The invention provides an active type soil-absorbed hydrocarbon sampling drill tool which comprises a drill tool main body; the drill tool main body comprises a drill rod adopting a hollow structure,a drill bit movably connected with the drill rod and adopting a hollow structure, a handle connected with the drill rod in a T-shaped manner and positioned on a side opposite to the drill bit, an absorbed hydrocarbon sampling device, a waterproof and breathable component, a sampling pipe and an air sampling device; collecting holes are formed in a tail end, opposite to the drill rod, of the drillbit; the absorbed hydrocarbon sampling device comprises a filter element positioned inside the hollow drill bit and connected with the collecting holes; the waterproof and breathable component positioned inside the hollow drill bit and connected with the filter element; the sampling pipe is positioned inside the hollow drill rod, one end of the sampling pipe is connected with the waterproof and breathable component, and the other end is connected with a collecting device; and the air sampling device is arranged in the air and connected with the collecting device through a pipe. According to the drill tool provided by the invention, the absorbed hydrocarbon sampling device is integrated with the drill tool, and various parameters can be monitored at the same time, and so that the integration level and the degree of automation are high.

The invention relates to the field of biotechnology and discloses a Loop type enrichment detection primer of low-abundance mutation DNA and application thereof. According to the Loop type enrichment detection primer disclosed by the invention, two short nucleotide sequences are connected by a Loop type structure, mutant type and wild type differential amplification is realized through the base accumulation effect, the concentration can be lowered to 10<-4> mutation enrichment to 1% to 10%, and the detection range of Sanger sequencing is reached so that the mutation enrichment can be combined with the Sanger sequencing to realize low-abundant mutation detection. The Loop type enrichment detection primer disclosed by the invention is suitable for detecting peripheral blood free DNA with lowmutation content, and realizes simple and convenient sampling while causing little pain of the patients; the detection technology has the advantages of high specificity, high sensitivity, easiness inoperation, short detection period, low detection cost and the like.

The invention discloses a method and device for multi-layer sampling of gas and water in a drill hole. The method comprises the following steps of: A, putting a water suction pump and a packer in a specified layer position in the drill hole; B, opening a nitrogen cylinder, adjusting a pressure reducing valve, injecting nitrogen into the packer through a setting pipe, and realizing setting of the packer; C, switching on a power supply, and starting the water suction pump for sampling; D, after sampling, switching off the power supply, turning off the water suction pump, and releasing nitrogen in the packer to realize deblocking of the packer; and E, lifting the packer and the water suction pump to the depth, and carrying out gas and water multi-layer sampling at the inner layer position ofthe drill hole. The water suction pump is connected with the packer through a drill rod, a water suction pump cable penetrates through the packer and is connected with the power supply; the packer isconnected with the pressure reducing valve and the nitrogen cylinder through the setting pipe, the packer is connected with a gas and water separation device through the drill rod; an upper outlet isconnected with a gas sampling bag; and a lower outlet is connected with a water sampling bottle. The device has the advantages of simple principle, clear structure, low cost, convenience in installation, simplicity and convenience in sampling, accurate and credible result and good application prospect.