Porcine reproductive and respiratory syndrome virus chimeric recombinant vaccine strain and method for producing living vaccine with same

A respiratory syndrome and recombinant vaccine technology, applied in the field of production of live vaccines for porcine reproductive and respiratory syndrome, can solve the problems of lack of immune protection, inability to protect wild virus strains, and strong virulence of live vaccines, and achieve good immune effects, The effect of protection against infection and stable genetic performance

Active Publication Date: 2009-12-16
CHINA INST OF VETERINARY DRUG CONTROL +2
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Problems solved by technology

[0025] Although there are many types of vaccines against PRRS, none of them can produce ideal safety protection in pigs
[0026] The research and development of porcine reproductive and respiratory syndrome (or porcine PRRS) vaccines is a worldwide problem. Live vaccines generally have the problem of strong virulence, while the main problem of inactivated vaccines is poor immune efficacy
At present, there is no satisfactory

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  • Porcine reproductive and respiratory syndrome virus chimeric recombinant vaccine strain and method for producing living vaccine with same

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[0051] The porcine reproductive and respiratory syndrome virus chimeric recombinant vaccine strain of the present invention uses reverse genetic manipulation and gene recombination technology to combine the second half of the GP2 gene of the classic porcine reproductive and respiratory syndrome virus (PRRSV) PTK strain and GP3~ After all the nucleotide sequences of the GP5 gene, M protein gene, N protein gene and their spacer sequence and 3'non-coding region are deleted, they are replaced with the corresponding genes of the Chinese highly pathogenic pig blue-ear disease variant strain to regroup into one A chimeric recombinant vaccine strain (PC strain) containing the genes of the classic and variant strains of porcine reproductive and respiratory syndrome at the same time, the corresponding gene of the Chinese highly pathogenic pig blue ear disease mutant strain is the Chinese highly pathogenic pig blue The second half of the GP2 gene of the ear disease variant strain, GP3 gene, ...

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Abstract

The invention relates to a porcine reproductive and respiratory syndrome virus chimeric recombinant vaccine strain and a method for producing living vaccine with same; latter half part of GP2 gene and GP3-GP5 gene of the porcine reproductive and respiratory syndrome virus (PRRSV) classic virus PTK strain, M ferritin, N ferritin and spacer sequences thereof and all nucleotide sequences of 3' non-coding region are all deleted by using reverse genetic manipulation and gene combination technology, then replaced with corresponding gene of Chinese highly pathogenic porcine reproductive and respiratory syndrome variant strain and are recombined to be the chimeric recombinant vaccine strain (PC strain) with the PRRSV classic strain and variant strain genes simultaneously, the chimeric recombinant vaccine PC strain is used to produce the living vaccine which has low toxicity, ensures no returning of strong toxicity within 5 generations of returning swine and the heritability is stable; the invention has good immunogenicity, and can prevent the porcine reproductive and respiratory syndrome virus classic strain and highly pathogenic variant strain from being infected simultaneously.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a method for constructing attenuated live vaccine strains by gene recombination technology and using the strains to produce live porcine reproductive and respiratory syndrome vaccines. Background technique [0002] Pig PRRS is a disease caused by porcine reproductive and respiratory syndrome virus (PRRSV), which is clinically characterized by fever, abortion, increased mortality of piglets before and after weaning, and respiratory disorders in pigs of different ages. In 1991, Dutch scholar Terpstra et al. isolated European-type PRRSV from infected pigs and named it Lelystad virus (Lv); in the same year, American scholar Benfield et al. isolated American-type PRRSV and named it VR-2332. In recent years, Chinese scholars have successively isolated and identified strains such as CH-1a, S1, HB-1 and HB-2 of the American PRRSV. [0003] Pig "high fe...

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Application Information

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IPC IPC(8): C12N7/01A61K39/12A61P31/14C12Q1/70C12Q1/68C12R1/93
Inventor 宁宜宝张学贤漆世华沈青春温文生刘业兵杨雷韩明远舒银辉张志学高和义
Owner CHINA INST OF VETERINARY DRUG CONTROL
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