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Molecular marker of holstein cattle heat resistant stress breeding and use thereof

A technology of Holstein cattle and heat stress resistance, applied in the field of molecular genetics, can solve the problems of heat shock protein 70 application, etc., achieve the effects of convenient and fast detection, early auxiliary selection, and low detection cost

Inactive Publication Date: 2009-05-06
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the application of heat shock protein 70 in the identification of heat stress resistance genes in Holstein cattle

Method used

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  • Molecular marker of holstein cattle heat resistant stress breeding and use thereof
  • Molecular marker of holstein cattle heat resistant stress breeding and use thereof
  • Molecular marker of holstein cattle heat resistant stress breeding and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1DNA extraction

[0030] Take blood samples, tissue samples or milk samples of the tested cows (choose one of the three samples), according to Jin Dongyan, etc. [1] Hetianyu [2] method to extract DNA from samples.

[0031] 2. Using the primers SEQ ID No. 1 and SEQ ID No. 2 screened in the present invention, PCR-SSCP analysis was performed on the extracted DNA and standard DNA, and non-denaturing polyacrylamide electrophoresis was performed on the amplified product. For gene polymorphism analysis, the specific operation process is as follows:

[0032] Use the above primers to perform PCR amplification on a PE 9600 amplicon: PCR reaction volume is 12.5 μl, of which 10×buffer 1.25 μl, 25 mM MgCl 2 0.8 μl, 1 μl of 2.5mM dNTPs, 0.25 μl of Taq enzyme (5u / μl), 100 ng of template DNA, 0.6 μl each of 20 μM primers, add water to 12.5 μl. The PCR reaction system was DNA pre-denaturation at 94 °C for 5 min, denaturation at 94 °C for 45 s, annealing at 60 °C for 30 s, extension...

Embodiment 2

[0038] Example 2: Type AA individuals have stable heredity.

[0039] According to follow-up studies, the parents of AA-type cows have A gene (both parents are homozygous for AA; or one is homozygous for AA and the other is heterozygous for AB or AC; or both All parties are AB or AC heterozygous), which proves that the AA type gene can be inherited. figure 2 .

Embodiment 3

[0040] Example 3: Relationship between different genotypes in the regulatory region of the HSP70 gene of Holstein cattle and heat stress resistance

[0041] 1. Gene frequency and genotype frequency of heat stress resistance (HSP70 gene regulatory region) loci in Holstein cattle

[0042] According to the assay method described above, a blood sample of Holstein cattle was collected to detect the polymorphism of the genetic marker. The results are shown in Table 1.

[0043] Table 1 Genotypes of Holstein cattle

[0044]

[0045] 2. Changes in rectal temperature, respiratory rate and milk production in cows of different genotypes

[0046] SPSS11.5 software was used to analyze the differences in rectal temperature, respiratory rate and milk production of Holstein cattle of different genotypes. The results are shown in Table 2 and Table 3.

[0047] Table 2 Rectal temperature and respiratory rate of each genotype of Holstein cattle

[0048]

[0049]

[0050] Note: Different ...

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PUM

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Abstract

The invention belongs to the field of molecular genetics, and relates to molecular marker of heat stress resistant breeding of a Hostein cattle and application thereof. The molecular marker is SEQ ID NO.3. The molecular marker can be used to assistantly breed the Hostein cattle with the characteristic of heat stress resistance and higher milk yield.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a molecular marker for heat stress resistance breeding of Holstein cattle and its application. Background technique [0002] In the high temperature (high humidity) environment, dairy cows can produce a series of physiological reactions, and the phenomenon of heat stress occurs, which is manifested as decreased appetite and reduced feed intake; increased body temperature and accelerated breathing; decreased nutrient absorption and utilization; Endocrine dysfunction leads to a significant decrease in the milk production of cows, prolonging the estrus cycle, shortening the estrus time, reducing the conception rate of cows and causing stillbirth; deterioration of bull sperm quality and reduced fertility; Reduced resistance will increase the incidence of obstetric diseases such as mastitis, endometritis, and afterbirth, which will have a great impact on the future reproduction and var...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/12A01K67/027
CPCY02A40/70
Inventor 王根林崔群维闫玉琴蔡亚非
Owner NANJING AGRICULTURAL UNIVERSITY
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