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Novel application of IFN-lambda in Zika virus infection

A virus infection and virus technology, applied in the direction of antiviral agents, medical preparations containing active ingredients, and resistance to media-borne diseases, etc., can solve the problems that the use has not been effectively developed

Active Publication Date: 2017-12-01
义乌国信白蚁防治有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of IFN-λ2 in Zika virus infection has not been reported yet
[0006] In the prior art, the use of IFN-λ2 in Zika virus infection has not been effectively developed, and it has been a long-term research topic in this field

Method used

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  • Novel application of IFN-lambda in Zika virus infection
  • Novel application of IFN-lambda in Zika virus infection
  • Novel application of IFN-lambda in Zika virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 IFN-λ2 can alleviate fetal mouse growth retardation caused by Zika virus

[0023] IFN-λ2 Treatment of Zika Virus Infected Pregnant Mice : pregnant mouse (IFNAR - / - ) On the 9.5th day of pregnancy (E9.5), 2 μg IFN-λ2 was injected, and on the 10.5th day of pregnancy (E10.5), the pregnant mice were infected with Zika virus. Then the pregnant mice were injected with the same dose of IFN-λ2 every day until the 18.5th day of pregnancy (E18.5). At E18.5, the size of the fetus was measured by crest-rump length × occipital-frontal diameter. Samples of various organs in the mother rat and fetal rat were collected for subsequent experiments.

[0024] test results : pregnant mouse (IFNAR - / -) on the 10.5th day of gestation (E10.5) infection with Zika virus can lead to intrauterine growth retardation in fetal mice. Pregnant mice were injected with IFN-λ2 (2 μg) before Zika virus infection (E9.5), and the same dose of IFN-λ2 was injected every day after Zika virus i...

Embodiment 2

[0025] Example 2 IFN-λ2 can reduce placental damage caused by Zika virus

[0026] HE staining : Rinse slides with tap water for 3 minutes. Rinse with distilled water for 1 min. The slides were permeabilized in 3% TritonX-100 solution pre-cooled at 4°C for 5 min. Hematoxylin staining for 10min. Rinse with tap water for 30s. The differentiation solution (2% hydrochloric acid ethanol) was treated for 20s. Rinse with tap water for 5-10 minutes. Eosin staining 30s ~ 2min. 50%, 70%, 80%, 95%, gradient dehydration with absolute ethanol for 1-3 minutes. Xylene I, II transparent treatment 1 ~ 3min. Mount the slides with neutral resin, and take images under a microscope.

[0027] test results : The placenta of normal mice is roughly divided into three layers: decidual layer (De), junctional zone (JZ), and labyrinthine zone (LZ). Attached from the manual figure 2 It can be seen from the figure that Zika virus infection can damage the normal structure of the placenta, and a...

Embodiment 3

[0028] Example 3 Inhibitory effect of IFN-λ2 on the replication of Zika virus in female mice and fetal mice

[0029] Collection of various organ tissues of the mother mouse described in Example 1 : Muscle (MCS), spleen (SPL), brain (BRN), heart (HRT), kidney (KDY), lung (LNG), lymph node (LYN), placenta (PLA) and liver (LIV), by q-PCR The relative Zika viral load in each organ of the dam was detected. Collect various organ tissues of fetal mice described in Example 1 : Muscle (MCS), brain (BRN), heart (HRT), kidney (KDY) and liver (LIV), the relative Zika virus load in each organ of fetal mice was measured by q-PCR.

[0030] Detection of Zika virus load : Take an appropriate weight of tissue and add Trizol to lyse it, extract RNA, and reverse transcribe it into cDNA with Oligo(dT)18 using a reverse transcription kit. Store at -80°C.

[0031] Primer sequences used to amplify Zika virus:

[0032] Forward: 5'-CCGCTGCCCAACACAAG-3';

[0033] Reverse: 5'-CCACTAACGTTCTTTTGCA...

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Abstract

The invention relates to a novel application of IFN-lambda in Zika virus infection and particularly provides an application of IFN-lambda 2 in preparation of a drug for preventing or treating Zika virus infection. Tests prove that delayed growth of fetal rats and placenta injury due to the Zika virus can be relieved by means of IFN-lambda 2, the relative Zika viral load in all organs of a mother rat and the fetal rats is detected through q-PCR (quantitative polymerase chain reaction), and the fact that IFN-lambda 2 can inhibit reproduction of the Zika virus in the mother rat and the fetal rats is further verified. Besides, the invention further illustrates a mechanism of the IFN-lambda 2 in inhibiting Zika virus reproduction by the means of cytobiology. A new way and method are provided for preventing or treating microcephalus caused by Zika virus infection.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a new application of IFN-λ in Zika virus infection, in particular to an application of IFN-λ2 in the preparation of drugs for preventing or treating Zika virus infection. Background technique [0002] Zika virus, also known as Zika virus, belongs to the family Flaviviridae and the genus Flavivirus. It is a single-stranded positive-sense RNA virus with a diameter of 20 nm. It is an arbovirus transmitted by mosquitoes. But unlike most arboviruses, Zika virus can directly infect and / or cross the placental barrier, causing tissue-specific damage. The virus was first discovered by chance in rhesus monkeys in the Zika jungle of Uganda in 1947 through the Yellow Fever Surveillance Network, and subsequently in Ugandan and Tanzanian populations in 1952. The earliest outbreak of the virus occurred in Yapu Island in the Micronesian Islands in the Western Pacific in 2007, and a larger epidemic occ...

Claims

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Application Information

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IPC IPC(8): A61K38/21A61P31/14
CPCA61K38/217Y02A50/30
Inventor 陈金铃季然段义农朱丹丹
Owner 义乌国信白蚁防治有限公司
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