Ultra-small nano immunopotentiator-antigen composite nanoparticle and preparation method and application thereof

A technology of composite nanoparticles and immune enhancer, which is applied in the directions of cancer antigen components, vertebrate antigen components, anti-tumor drugs, etc., can solve the problems of difficult to treat metastatic malignant tumors, unable to achieve ideal tumor immune effect, etc., and achieves the preparation method. Simple, immunogenic, and biocompatible

Pending Publication Date: 2020-12-11
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] According to one aspect of the present application, an ultra-small nano-immunity enhancer-antigen composite nanoparticle and its preparation method are provided, which avoids the shortcoming that the ideal tumor immune effect cannot be achieved caused by the weak immunogenicity of the free antigen and solves the current problem. Metastatic malignant tumors are difficult to treat, achieving the dual purpose of immunotherapy and prevention of metastatic malignant tumors

Method used

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  • Ultra-small nano immunopotentiator-antigen composite nanoparticle and preparation method and application thereof
  • Ultra-small nano immunopotentiator-antigen composite nanoparticle and preparation method and application thereof
  • Ultra-small nano immunopotentiator-antigen composite nanoparticle and preparation method and application thereof

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Embodiment 1

[0070] First prepare ferric oxide nanoparticles, 3.4g FeCl 3 ·6H 2 O was dissolved in 12ml of deionized water, and then 10.5g of sodium oleate was added to obtain a reaction mixture. A mixture of 45ml of n-hexane and 25mL of ethanol was added to the reaction mixture and heated to 70°C for 4 hours. After the reaction, the top organic layer (ie ferric oleate) was obtained through a separatory funnel, and washed three times with deionized water. The n-hexane was removed by low-speed rotary evaporation to obtain iron oleate nanoparticles.

[0071] Fe3O4 nanoparticles were obtained by ferric oleate, oleyl alcohol and octadecenoic acid. Dissolve 2g of iron oleate complex in 20ml of oleyl alcohol and 0.3ml of octadecenoic acid mixture. After degassing with nitrogen, reflux at 300° C. for 60 minutes under nitrogen protection. The solution turned from brown to black. After cooling to room temperature, acetone was added to stabilize the nanoparticles, which were isolated by centri...

Embodiment 2

[0076] Firstly, ferric oxide nanoparticles were prepared, and FeCl 3 ·6H 2 O was dissolved in deionized water, followed by the addition of sodium oleate. The mixture of n-hexane and ethanol was added to the reaction mixture and added to 70° C. for 4 hours. After the reaction, the top organic layer (ie ferric oleate) was obtained through a separatory funnel, and washed three times with deionized water. The n-hexane was removed by low-speed rotary evaporation to obtain iron oleate nanoparticles.

[0077] Fe3O4 nanoparticles were obtained by ferric oleate, oleyl alcohol and octadecenoic acid. Dissolve 2g of iron oleate complex in oleyl alcohol and octadecenoic acid mixture. After degassing with nitrogen, reflux at 300° C. for 60 minutes under nitrogen protection. The solution turned from brown to black. After cooling to room temperature, acetone was added to stabilize the nanoparticles, which were isolated by centrifugation and dissolved in cyclohexane.

[0078] Mix polyst...

Embodiment 3

[0082] First prepare iron ferric oxide nanoparticles, purify 20mL polyacrylic acid aqueous solution (M w=1800, the concentration is 5 mg / mL) for 60 minutes to remove oxygen, and then the solution was heated to reflux at 100°C. Subsequently, 0.4 mL of ferric chloride (500 mM) and 500 mM of ferric sulfate were quickly added to the solution, and 6 mL of ammonia water (28%) was added, and magnetic stirring was continued at 100° C. After 1 hour, the solution was cooled to room temperature. Obtain ultra-small iron ferric oxide nanoparticles, and dialyze it in ultrapure water environment for 5 days (cutoff molecular weight is 6-8kDa). Then the solution after dialysis is subjected to ultrafiltration and centrifugation (the cut-off molecular weight is 10kDa), and the ultra-small iron ferric oxide nanoparticles obtained after concentration are denoted as sample Fe10 # .

[0083] Subsequently, take 20 μg of the prepared ferric oxide nanoparticle powder and dissolve it in deionized wat...

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Abstract

The present invention discloses an ultra-small nano immunopotentiator-antigen composite nanoparticle and a preparation method and an application thereof. The composite nanoparticle comprises a nano material and an antigen. The composite nanoparticle avoids a defect that an ideal tumor immune effect cannot be achieved due to weak immunogenicity of the free antigen, solves a problem that metastaticmalignant tumors are difficult to treat at present, and achieves dual purposes of immunotherapy and prevention of the metastatic malignant tumors.

Description

technical field [0001] The application relates to an ultra-small nanometer immunopotentiator-antigen composite nanoparticle and its preparation method and application, belonging to the field of material preparation methods and applications. Background technique [0002] According to the forecast of the World Health Organization, there will be 20 million new cases of tumors in 2020, including 12 million deaths. Tumors will become the number one killer of human beings in the new century. Malignant tumors are highly aggressive and prone to local or distant metastases, especially lung metastases. Existing treatments are almost ineffective for it, resulting in many cancer patients dying of tumor metastasis. The treatment and prevention of metastatic malignant tumors has always been a major difficulty in the field of tumors. Based on its advantages, tumor immunotherapy has made it a possible and effective solution. [0003] Tumor immunotherapy usually regulates the body's anti-t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K39/39A61K9/51A61K47/02A61P35/00
CPCA61K9/5115A61K39/0011A61K39/39A61P35/00
Inventor 吴爱国罗利嘉
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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