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Application of macromolecular protein in anti-RNA virus disinfectant

A technology of RNA viruses and macromolecules, applied in the field of anti-RNA virus disinfectants of macromolecular proteins, can solve the problems of few studies on physical and chemical stability, protein instability, variability, etc.

Inactive Publication Date: 2020-12-22
武汉愔紫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason is that there are very few studies on the physical and chemical stability of these RNases in the prior art, and common sense believes that the enzyme protein needs to be within the normal body temperature range of humans to exert the enzyme function and activity, and the protein is unstable in the natural environment and easily denatured , corruption and degradation

Method used

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  • Application of macromolecular protein in anti-RNA virus disinfectant
  • Application of macromolecular protein in anti-RNA virus disinfectant
  • Application of macromolecular protein in anti-RNA virus disinfectant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Disclosed is a disinfectant for RNA viruses that acts on the outside or body surface, and the active ingredient of the disinfectant is recombinant RNase2 and / or RNase3.

[0046] Recombinant RNase2 and recombinant RNase3 are recombinant human-derived proteins, which can be expressed and purified by recombinant DNA from E.coli (Escherichia coli), Sf9 insect cells or human 293 cells, and the purity of the protein is greater than 90%, and the concentration is 1~ 200 μM. The preparation of recombinant RNase2 and recombinant RNase3 is a prior art, and will not be repeated here.

[0047] Water is used as a solvent, and the concentration of recombinant RNase2 and / or RNase3 is 0.01-5000nM.

[0048] In order to maintain long-term activity and improve the use effect of the disinfectant, protein stabilizers, phosphate buffers, and preservatives can also be added to the disinfectant. The protein stabilizer includes one or more of antiseptic and antibacterial protein stabilizers, p...

Embodiment 2

[0052] Stability verification of embodiment 2 disinfectant

[0053] When the anti-RNA virus disinfectant in Example 1 is applied to the body surface or in vitro, it should first be ensured that the recombinant RNase2 and recombinant RNase3 have sufficient stability, can be properly stored for a long time, and can not be easily destroyed by the natural environment in various environments. It can be rapidly destroyed, degraded and inactivated by conditions and conditions, and can be mixed with other auxiliary ingredients.

[0054] (1) Thermal stability of recombinant RNase2 and recombinant RNase3

[0055] Under various temperature conditions, the ribonuclease activity of RNase2 was tested. Ribonuclease assay: catalytic reaction Add 40mg of yeast tRNA as substrate to 0.8mL reaction solution (containing 40mM sodium phosphate, pH 7.0 and 10mL rhRNase2, or rRNaseH, or buffer control). Reactions were terminated by adding 40 nM lanthanum nitrate in ice-cold 3% perchloric acid at giv...

Embodiment 3

[0069] The anti-RNA virus ability of embodiment 3 disinfectant

[0070] Treat the same amount of respiratory syncytial virus and parainfluenza virus (PIV) with different doses of recombinant RNase2, then use the treated virus to infect the cultured cells, and detect the number of active viruses. The results are shown in the table below (all experiments were repeated 3 times, each 2 times, the result is the mean, ± standard deviation):

[0071]

[0072] It can be seen from the above table that the number of active viruses decreases with the increase of the amount of RNase2, which proves that RNase2 has inhibitory and destructive effects on both viruses, but the sensitivity of the viruses to RNase2 is different. Its inferred reason is: respiratory syncytial virus and parainfluenza virus all have Matrix Proteins (such as Figure 8 shown), is a key protein in the structure of the virus, and plays an important role in assembling the virus, protecting the viral envelope and geno...

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Abstract

The invention provides an application of macromolecular protein in an anti-RNA virus disinfectant. The macromolecular protein is one or more of natural immune polypeptide and protein secreted by bacteria, fungi, animals or plants in the biological field, the disinfectant acts on the body surface environment, the in-vitro environment or the oral and nasal cavity environment, and the macromolecularprotein is human RNase 2 and / or RNase 3. The RNase 2 and RNase 3 in the disinfectant are very stable in structure and lasting in activity, can inhibit viruses in vitro and directly destroy virus outermembranes, and meanwhile can degrade virus genomes, and can reduce the infection virus load in the in-vitro environment; compared with the existing small molecule disinfectant, the disinfectant has the advantages of long action time, high efficiency, no irritation to human bodies, environmental protection, cyclic effect exertion and the like, and is expected to have great application prospects inthe aspect of prevention of respiratory tract and gastrointestinal tract RNA virus infection of novel coronavirus, influenza virus and the like.

Description

technical field [0001] The present invention relates to the technical field of disinfection products, in particular to the application of a macromolecular protein in an anti-RNA virus disinfectant. Specifically, the space and scope of human innate immunity are expanded by using recombinant human natural immune molecule RNase products on the human body surface and peripheral environment , to achieve disease prevention. Background technique [0002] Ribonucleases (RNases) are RNA hydrolases that are produced, secreted, and widespread in nature. The RNase A superfamily is a protein family specific to vertebrates, covering eight functional members of humans. These proteins are secreted by various innate immune cells ranging from myeloid cells to epithelial cells, and their levels in our body are associated with infection and inflammatory processes, due to the important role of secreted RNases in the extracellular space of the body. Among these 8 enzymes, some have disinfection ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/46A61P31/14A01N63/50A01N37/46A01P1/00
CPCA01N37/46A61K38/465A61P31/14A01N63/50C12Y301/26C12Y301/26003
Inventor 朱小湘
Owner 武汉愔紫生物科技有限公司
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