Culture medium and method for constructing in-vitro 3D cell model of skin tissue

CN119307439AActive Publication Date: 2025-01-14SUZHOU GENOARRAY
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Patent Information

Application Number
CN202411832617.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-13
Publication Date
2025-01-14
Estimated Expiration
2044-12-13

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Abstract

The invention discloses a culture medium and a method for performing in-vitro 3D cell model construction on skin tissues. The invention provides a culture medium for constructing a skin tissue in-vitro 3D (three-dimensional) cell model. The culture medium is prepared from an antibacterial and antifungal agent tri-antibody, HEPES, GlutaMax, MEM non-essential amino acid, human recombinant protein EGF, bFGF, HGF, FGF-10, Wnt-3a, Noggin, R-spondin 1, IL-2, IL-15, IGF-I, CHIR99021, SB202190, A83-01, Primocin, N-acetyl-L-cysteine, nicotine, an N2 additive, B27, ITS-X, Y-27632, dexamethasone, calomel and a basal culture medium. The skin tissue in-vitro 3D cell model constructed by the invention has a wide application prospect in the fields of basic scientific research and clinical diagnosis and treatment of skin diseases.
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Citation Information

Patent Citations

  • Miniature skin structure, preparation method and use thereof

    CN101912636A

  • Method for regenerating human intact skin tissue by use of in vitro cultured cells

    CN103785064A

  • 3D full-layer skin model, culture medium for forming 3D full-layer skin model and preparation method of 3D full-layer skin model

    CN109722410A

  • Method for obtaining sweat gland organs from separation and culture of sweat gland cells and application thereof

    CN109943520A

  • In-vitro culture medium for skin tissue and application

    CN111826337A

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