Julans sigillata Dode proline-enriched protein gene JsPRP1 and applications thereof

A technology of Yangbi big bubble walnut and proline protein, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve problems such as differences in expression characteristics, and achieve the effects of shortening the breeding cycle, reducing environmental pollution, and reducing the use of
CN104774847AActive Publication Date: 2015-07-15KUNMING UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
KUNMING UNIV OF SCI & TECH
Publication Date
2015-07-15

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Abstract

The invention discloses Julans sigillata Dode proline-enriched protein gene JsPRP1 and applications thereof. The nucleotide sequence of the JsPRP1 is represented by the SEQ ID No.1, and the encoding is rich in proline protein. Through the related technologies and researches of functional genomics, the results show that JsPRP1 gene has a function that can improve the plants' performance on resisting fungal infection. The anti-fungus gene JsPRP1 can be built on an expression carrier and over-expressed in tobacco, and the transgene tobacco plants has a strong in-vitro activity on resisting fungi. The transgene tobacco, where the JsPRP1 gene is over-expressed, has a prominent inhibiting effect on the growth of colletotrichum gloesporioides, sclerotinia sclerotiorum, botryosphaeria, and catenulate gibberella.
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Description

technical field

[0001] The invention relates to the field of molecular biology and genetic engineering related technology research, in particular to the proline-rich proline protein gene of Yangbio walnut with antifungal activity JsPRP1 and applications. Background technique

[0002] Plants may be infected by pathogenic bacteria throughout the growth period. Among the various plant diseases, fungal diseases account for about 70-80% of plant diseases. The rise of classical genetics in the early 20th century has enabled people to successfully breed new disease-resistant varieties through cross-breeding, thereby greatly increasing food production. However, conventional breeding has the disadvantages of long cycle, time-consuming and labor-intensive, and few beneficial mutations, which cannot fundamentally solve the problem of plant diseases. In recent years, with the continuous development of molecular biology theory and technology, people can not only deeply understand the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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