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Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease

A technology for dissolving starch spores and peach liquid gum, applied in the application, bacteria, fungicides and other directions, can solve the problems such as microorganisms or biological preparations that have not been reported, and achieve significant control effects, high inhibitory activity, and reduced morbidity.

Active Publication Date: 2013-12-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are microorganisms or biological agents developed for the prevention and treatment of plant diseases, and some control effects have been achieved, but there have been no reports on microorganisms or biological agents specifically for the prevention and treatment of peach gummosis

Method used

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  • Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease
  • Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease
  • Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1 Isolation, screening and identification of Bacillus amyloliquefaciens H47CCTCCM2013283

[0025] 1.1 Isolation and screening of Bacillus amyloliquefaciens H47CCTCCM2013283

[0026] (1) Raw raw eucalyptus honey samples were collected from Fuxu County, Guangxi, China in March 2012;

[0027] (2) Dilute the above-mentioned raw honey sample to 75% (w / w) with sterilized normal saline, take 200 μL and spread it on LB agar medium, place it in an incubator for 72 hours at 30°C, and pick out a single colony that grows Streak on fresh LB plate medium for separation and purification, culture at 30°C for 48 hours, then pick a single colony to LB slant medium, and store at 4°C.

[0028] (3) Inoculate the pathogenic bacteria of peach gum disease, Botrytis aureus, on the PDA medium, and culture it at 25-30°C for 4-6 days; cut off the 5-10mm bacterial cake from the edge of the colony of Botrytis aureus, and inoculate it on the newly prepared PDA plate for culture base center;...

Embodiment 2

[0041] Example 2 Detection of the antagonistic effect of Bacillus amyloliquefaciens H47CCTCC M2013283 strain on Botrytis

[0042] Inoculate the Botrytis preserved on the slant on the PDA plate medium, and activate it under the light condition of 30°C for 7 days. After the Botrytis is overgrown on the plate, the culture is terminated, and the sterilized hole puncher with a diameter of 12.00mm is used for activation. Make a fungus cake at the edge of the pathogenic bacteria colony, put the fungus cake face up on one side of the confrontation culture plate, about 1cm close to the wall of the dish, pick a ring of Bacillus amyloliquefaciens H47CCTCC activated on the LB slant medium for 24 hours with an inoculation needle M2013283, draw a bacterial line parallel to the bacterial block in the confrontation plate, put the confrontation culture plate into a 30°C incubator for cultivation, and record the width of the inhibition zone after 5 days.

[0043] Test results: The width of the ...

Embodiment 3

[0044] Example 3 Preparation of Bacillus amyloliquefaciens H47CCTCC M2013283 Fermentation Liquid and Anti-Gum Disease Biological Agent

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Abstract

The invention discloses bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding diseases. The collection number of the Bacillus amyloliquefaciens H47 is CCTCC NO: M2013283. A biological agent which resists the peach bleeding diseases is prepared by carrying out liquid fermentation on the strain can be applied to peach trees; the liquid fermentation process comprises the following steps of: slant strain activation, seed culture and liquid deep fermentation culture. The strain disclosed by the invention and thallus-free spore-free fermentation liquor can both generate a higher antagonistic effect on the pathogen botryosphaeria of the peach bleeding diseases; the biological agent disclosed by the invention achieves an outstanding preventing and controlling effect on peach tree bleeding diseases; compared with the traditional chemical agent, the biological agent disclosed by the invention has the advantages of greenness, safety, environment friendliness, and the like and has wide application prospect on the aspect of biological prevention and control of the bleeding diseases.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and fermentation, and in particular relates to a strain of bacillus amyloliquefaciens and the application of the strain in preventing and treating peach gum disease. Background technique [0002] Peach tree gummosis, also known as tumor skin disease or skin disease, is a disease that endangers peach trees worldwide. Peach gummosis is common in peach orchards in my country and the damage is serious. The disease can cause gelatinous overflow on the trunk and main branch bark of the peach. The overflow is colorless and transparent at the beginning, and then turns yellowish brown to dark brown, and a large amount of colloid overflows. The peach trees are weakened, which in turn leads to a decline in fruit yield and quality. In severe cases, the branches are dead and broken, and even the whole tree dies. Before the 1970s, peach gummosis was considered to be a physiological disease of peaches. Un...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 黎循航廖祥儒赵晓联蔡宇杰管政兵
Owner JIANGNAN UNIV
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