Primer pair and detection method for physalospora piricola virus detection

A technology of Pear rota bacteria and detection primers, applied in the field of microbial detection, can solve the problems of cumbersome procedures, interference, difficulty in timely monitoring the spread of pathogenic bacteria and disease prevalence, etc., and achieves high specificity, short time-consuming and high sensitivity. Effect

Inactive Publication Date: 2016-01-20
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology

However, direct observation will miss the situation that does not show symptoms in the early stage of the disease, and the morphological detection method is easily disturbed by human factors and environmental conditions. In addition, the traditional classification method is time-consuming and cumbersome, and is not suitable for the requirements of rapid detection. Realize the timely monitoring of the occurrence of diseases and effectively control the spread of pathogenic bacteria and the prevalence of diseases

Method used

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  • Primer pair and detection method for physalospora piricola virus detection
  • Primer pair and detection method for physalospora piricola virus detection
  • Primer pair and detection method for physalospora piricola virus detection

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Effect test

Embodiment 1

[0017] Example 1 Primer Design

[0018] According to the differences in the ITS sequences of different species of R. pirifera registered in GenBank, primers BsF1 / BsR2 specific to R. pirifera were designed with the Primer5 software. The designed sequence is:

[0019] BsF1: 5'-CTTTGTGTACCTACCCTCTGTTGCTTTGG-3';

[0020] BsR2: 5'-GTGCCCAATACCAAGCAGAGCT-3'.

Embodiment 2

[0021] Embodiment 2 detection method

[0022] 2.1 The tested strains are shown in Table 1

[0023] Table 1 Tested strains

[0024] strain the host source Number of strains B. berengeriana Pyrus sp. Jiangsu 9 B. berengeriana Pyrus sp. Anhui 4 B. berengeriana Pyrus sp. Shaanxi 3 B. berengeriana Pyrus sink angensis yü Xinjiang 2 B. berengeriana Pyrus sp. Henan 1 B. berengeriana Pyrus sp. Shandong 1 B. berengeriana Malus sp. Shandong 2 B. parva Eucalyptus globulus Chinese Academy of Forestry 1 B. rhodina Eucalyptus sp. Chinese Academy of Forestry 1 B. eucalyptorum Albizia falcalaria Chinese Academy of Forestry 1 Colletotrichum gloeosporioides Pyrus sp. Henan 1 Alternaria alternata Pyrus sp. Jiangsu 1 Valsa ambiens Pyrus sp. Nanjing Agricultural College 1 Phoma sp. Pyrus ...

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Abstract

The invention relates to a rapid physalospora piricola virus detection technique. According to difference of different ITS sequences of physalospora piricola virus registered in GenBank, specific physalospora piricola virus primers BsF1 / BsR2 are designed by using Primer5 software, the primers can only amplify a 332bp strip specifically from only 22 test Botryosphaeria / berengeriana strains, and not any strip is amplified from other strains and blank control strains. The invention further provides a detection method of the primer for the physalospora piricola virus and can detect a sample DNA with lag quantity. The detection method has the advantages of good specificity, high sensitivity, short time, and the like, and plays an important role in in-time detection and effective control on diseases.

Description

technical field [0001] The invention relates to the technical field of microbial detection, in particular to a primer pair and a detection method for detection of R. Background technique [0002] Pear ring spot, also known as rough skin disease, is widely distributed in various pear producing areas in my country and is one of the important diseases of pear trees in my country. The disease mainly harms the branches, fruits and leaves of pear trees. Damage to branches can cause premature senescence of branches, damage to leaves can cause premature leaf fall, and damage to fruits can cause a large number of rotten fruits, seriously affecting yield and quality. In our country, pear ring spot disease causes about 25% pear fruit production reduction every year, and the rotten fruit rate exceeds 80% in severe cases. Pear ringworm ( Botryosphaeria berengeriana ) has a wide range of hosts. In addition to pears, it can also harm various fruit trees such as apples, peaches, plums, ap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12R1/01
CPCC12Q1/689
Inventor 刘凤权赵延存何锋
Owner JIANGSU ACAD OF AGRI SCI
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