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Fusarium equiseti strain for preventing and treating poplar canker and application thereof

A technology for poplar canker and Fusarium equisetum, applied in the field of plant protection, can solve problems such as destruction and environmental pollution, and achieve the effects of simple cultivation conditions, easy industrial production, and good development and application prospects

Active Publication Date: 2015-10-14
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the regulation of the spatial structure of the ecosystem is a relatively long process, and the pollution and destruction of the environment by chemical control has become a problem that cannot be ignored.

Method used

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  • Fusarium equiseti strain for preventing and treating poplar canker and application thereof
  • Fusarium equiseti strain for preventing and treating poplar canker and application thereof
  • Fusarium equiseti strain for preventing and treating poplar canker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation, screening and identification of poplar canker biocontrol bacteria in soil

[0024] The dilution plate coating method was used to separate soil microorganisms. Place 1g of soil in a 250mL Erlenmeyer flask containing 100mL of sterile water to make a mother liquor of soil suspension. Place the mother liquor in a shaker at 28°C and shake for 1 hour. Under aseptic conditions, perform a 10-fold gradient dilution of the soil mother liquor, with a dilution factor of 10 -2 ~10 -7 The bacteria are separated by soil suspension with a dilution gradient of 10-5~10-7, actinomyces are separated by a soil suspension with a dilution gradient of 10-3~10-5, and fungi are separated by 10-2~10-4. Dilute gradient soil suspension for separation. Based on the culture characteristics and colony characteristics, it was determined that 122 strains of microorganisms were isolated from the upper, middle and lower layers of soil. Through the plate confrontation experiment, 69 str...

Embodiment 2

[0027] Example 2 Optimization and stability of fermentation process of Fusarium equisetum CGMCC8795 strain

[0028] Through single factor experiment, orthogonal design and response surface analysis, the optimal fermentation medium and conditions for CGMCC8795 were finally determined as follows: potato extract 200g / 1000mL, fructose 1%, beef extract 1.4%, KCl 0.1%, start The pH value is 7.0, the temperature is 32°C, the rotation speed is 180r / min, the inoculation volume is 4%, and the liquid volume is 100mL / 250mL. After optimization, the antibacterial rate of the strain CGMCC8795 can reach 75.88%, which improves the antibacterial activity of the biocontrol strain and provides a guarantee for the subsequent development and utilization of the strain.

[0029] Fusarium equisetum CGMCC8795 has a certain tolerance to acid-base treatment. Under the condition of pH 1, the bacteriostatic rate is 47.28%, and under the condition of pH 12, the bacteriostatic rate can still be 41.57%. It is pro...

Embodiment 3

[0030] Example 3 Preparation of Fusarium equisetum CGMCC8795 biocontrol agent powder

[0031] Solid fermentation method is used to prepare the original powder of bacteria. Set up 3 different solid fermentation substrate formulas: I. solid material 400g, of which 50% broken rice, 50% wheat bran, and the ratio of solid material to water is 1:1 (W / V), II. solid material 400g, of which Broken rice 50%, corn flour 50%, and the ratio of solid material to water is 1:1 (W / V), III. Solid material 400g, of which 50% wheat bran, 50% corn flour, and the ratio of solid material to water It is 1:1 (W / V). The results show that it is most suitable to use 50% corn flour and 50% wheat bran as the solid fermentation substrate, the antibacterial rate is 90.59%, and the number of viable spores reaches 4.69×10 11 cfu / g.

[0032] The selection of solid fermentation conditions adopts an orthogonal design with three factors and three levels, and a total of 9 sets of experiments are set up. The final sui...

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Abstract

The invention discloses a biocontrol strain for preventing and treating poplar canker and an application method thereof. The strain is fusarium equiseti, with number of CGMCC 8795. Fermentation liquor of the strain is mixed with wheat bran and corn powder, the product is naturally air-dried and ground to prepare raw powder after fermentation for seven days, and 42wt% of raw powder, 36wt% of kaolin, 12wt% of Tween 20, 8wt% of nekal and 2wt% of ascorbic acid are added to prepare an agent. After being diluted, the agent is used for preventing and treating the poplar canker by spraying and root irrigation. The strain has the advantages of various modes of action on pathogenic bacteria, high reproduction speed, stable action effects, and the like, has strong inhibiting effects on the pathogenic bacterium botryosphaeria dothidia of the poplar canker, is of great importance in prevention and control of the poplar canker and has good development and application prospects.

Description

Technical field: [0001] The invention relates to the field of plant protection, in particular to a biocontrol bacteria strain for preventing and treating poplar ulcer disease and its application, in particular to a highly effective biocontrol strain of Fusarium equisetum CGMCC8795 for preventing and treating poplar ulcer disease and its application. technical background: [0002] Poplar and its hybrid varieties are widely distributed around the world. It can not only be used as an important processing material for wood-based panels and fiber materials, but also play an important role in afforestation, ecological restoration, landscape greening, and wind and sand fixation. Poplar canker (sexual pathogenic bacteria Botryosphaeria dothidia, asexual microcystis Dothiorella grearia) is the main type of poplar stem disease in China. It is distributed in the poplar cultivation areas in North China, Northwest and South China. There are more occurrences in young forests and serious damage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/77
CPCA01N63/30C12N1/145C12N1/205C12R2001/14C12R2001/77
Inventor 梁军杨蕾倪杨张星耀
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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