15 results about "Bacillus enteritidis" patented technology

The application discloses an Escherichia coli probiotic with high beta-alanine yield and application thereof, and the recombinant genetically engineered bacterium takes a wild-type Escherichia coli probiotic as a starting strain and is subjected to one or more gene edits, including Trc promoter strengthening of a panD gene, an aspC gene, an sdhC gene and a ppc gene, overexpression of a panD gene from Bacillus subtilis, an aspB gene from Corynebacterium glutamicum, an aspA gene and a pyc gene from Corynebacterium glutamicum on a plasmid pTrc99a, and knockout of a cycA gene. 600 Compared with an existing reported Escherichia coli probiotic beta-alanine production strain, the OD of the recombinant genetically engineered bacterium is 4.4 times that of the existing reported strain, and the beta-alanine yield is 6 times that of the existing reported strain, so that the recombinant genetically engineered bacterium has great industrial application potential.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

This invention discloses the antimicrobial peptide PJX13 and its application in the control of citrus canker. The sequence of the antimicrobial peptide PJX13 is AcKKFWKLRRWVKWIGNH2. It can effectively inhibit and kill *Xanthomonas citrus* by disrupting the cell membrane integrity of pathogens, leading to leakage of intracellular substances. It effectively inhibits the expansion of lesions on both detached citrus leaves and living plants, and can be formulated into an antimicrobial product against *Xanthomonas citrus* for the control of citrus canker. Furthermore, the antimicrobial peptide PJX13 can also inhibit *Escherichia coli*, *Staphylococcus aureus*, and *Bacillus oryzae*, and can be used to prepare broad-spectrum antimicrobial products. This invention is beneficial for the control of citrus canker, and has advantages such as being environmentally friendly and unlikely to induce drug resistance.

The application discloses a composite biological deodorant, which comprises the following components in parts by weight: 1-3 parts of Bacillus coagulans slurry; 1-3 parts of Saccharomyces cerevisiae slurry; 1-3 parts of Lactobacillus plantarum slurry; 10-30 parts of nutrient solution A; and 20-60 parts of nutrient solution B. The composite biological deodorant is prepared by using the composite system of the Bacillus coagulans slurry, the Saccharomyces cerevisiae slurry and the Lactobacillus plantarum slurry, and combining with a specific nutrient solution carrier. The Bacillus coagulans provides environmental tolerance guarantee for the composite system due to strong environmental resistance. Metabolic products secreted by the Saccharomyces cerevisiae help to promote the proliferation of the Lactobacillus plantarum. Bacteriocins produced by the Lactobacillus plantarum selectively inhibit staphylococcus aureus, escherichia coli and other foul-smelling pathogenic bacteria, and degrade small-molecule foul-smelling substances such as ammonia nitrogen and hydrogen sulfide. The three components are synergistic, so that the deodorization and bacteriostasis effects are achieved. The product has no strong peculiar smell, and the risk of contamination by miscellaneous bacteria is effectively avoided.

The invention discloses an antibacterial peptide NyAmp-a and application thereof, and relates to the technical field of biological medicines. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID NO. 1. The invention also provides an application of the antibacterial peptide NyAmp-a in preparation of an antibacterial product for inhibiting staphylococcus aureus, bacillus subtilis, escherichia coli and aeromonas hydrophila, and a minimum inhibitory concentration of the antibacterial peptide NyAmp-a to each bacterium. The invention provides a novel efficient antibacterial solution for four common pathogenic bacteria, namely staphylococcus aureus, bacillus subtilis, escherichia coli and aeromonas hydrophila. The antibacterial peptide NyAmp-a has the characteristic that drug resistance is not easy to generate, a related antibacterial composition can be applied to the fields of medicine, agriculture, food and the like, has important practical application value, and effectively solves the problems that traditional antibiotics are easy to generate drug resistance, and existing antibacterial peptides are high in cytotoxicity and lack of selectivity for specific strains.

The invention discloses bacillus velezensis LYBV022 and application thereof in preparation of a preparation for inhibiting clostridium perfringens infection, and belongs to the technical field of microorganisms. The invention discloses a bacillus velezensis LYBV022, and the preservation number of the bacillus velezensis LYBV022 is GDMCC No: 66952. The bacillus velezensis LYBV022 disclosed by the invention has a relatively good antibacterial ability on clostridium perfringens, staphylococcus aureus, escherichia coli and the like, so that the bacillus velezensis LYBV022 has a clinical application potential.

The invention discloses a composite biological deodorant which comprises the following components in parts by weight: 1-3 parts of bacillus coagulans bacterial sludge; 1-3 parts of saccharomyces cerevisiae mud; 1-3 parts of plant lactobacillus bacterial sludge; 10-30 parts of a nutrient solution A; and 20-60 parts of a nutrient solution B. The composite biological deodorant is prepared by innovatively adopting a compound system of bacillus coagulans bacterial sludge, saccharomyces cerevisiae bacterial sludge and plant lactobacillus bacterial sludge and combining with a specific nutrient solution carrier, and bacillus coagulans provides environmental tolerance guarantee for a compound system by virtue of strong environmental resistance; metabolites secreted by the saccharomyces cerevisiae are beneficial to promoting proliferation of the plant lactobacillus, and bacteriocin generated by the plant lactobacillus selectively inhibits staphylococcus aureus, escherichia coli and other malodorous pathogenic bacteria and degrades ammonia nitrogen, hydrogen sulfide and other micromolecular malodorous substances at the same time; the three components have a synergistic effect, so that a remarkable deodorization and bacteriostasis effect is achieved, the product is free of strong peculiar smell, and the risk of infectious microbe pollution is effectively avoided.

The invention belongs to the technical field of chemical synthesis of medicines, and particularly discloses a 1, 4-benzodioxane thiourea compound as well as a preparation method and application thereof, the structural general formula of the 1, 4-benzodioxane thiourea compound is as follows: in the formula, R group is methyl, methoxyl or halogen group. The 1, 4-benzodioxane thiourea compound has an inhibiting effect on at least one of staphylococcus aureus, bacillus subtilis, escherichia coli and pseudomonas aeruginosa, the synthetic route is simple, the used raw materials are cheap and easy to obtain, the yield is high, and the 1, 4-benzodioxane thiourea compound is suitable for industrial production and is an antibacterial drug with huge potential.

The application belongs to the technical field of industrial circulating water treatment, and provides a green water treatment agent with corrosion inhibition and bacteriostasis performance, a preparation method and application, the water treatment agent has the structure as shown: The application introduces a piperazine group into a polyaspartic acid peptide bond structure by a graft copolymerization method, synthesizes a novel water treatment agent, the water treatment agent can be adsorbed on a metal surface to form a protective film, has good corrosion inhibition and bacteriostasis performance. The water treatment agent also has bacteriostasis specificity, can inhibit escherichia coli, and retains bacillus cereus. Therefore, the application realizes efficient improvement of the corrosion inhibition performance and bacteriostasis performance of polyaspartic acid, reduces the application of phosphorus-containing compounds, and avoids the influence on the environment.

The application discloses mulberry leaf volatile oil and a preparation method and application thereof, and relates to the technical field of plant extraction. The preparation method of the mulberry leaf volatile oil comprises the following steps: (1) crushing mulberry leaves to obtain mulberry leaf powder; (2) mixing the mulberry leaf powder with deionized water, and then using a microwave-assisted hydro-distillation (MAHD) method to obtain a distillate; and (3) using an organic solvent to extract the distillate to obtain the mulberry leaf volatile oil. The mulberry leaf volatile oil is extracted from mulberry leaves by using the MAHD method, the extraction process is optimized through single-factor experiments and a response surface design, and the optimal extraction process is obtained. It is verified that the mulberry leaf volatile oil prepared by the application has antibacterial activity on bacillus subtilis, salmonella paratyphi B, pseudomonas aeruginosa, escherichia coli and staphylococcus aureus, and has a strong scavenging capacity on ABTS and DPPH free radicals.

The invention belongs to the technical field of microorganisms, and relates to a bifidobacterium lactis BL-PG-09 strain capable of relieving diarrhea and a preparation method of the bifidobacterium lactis BL-PG-09 strain, the strain is bifidobacterium lactis BL-PG-09, the preservation number is CGMCC No.30076, the preservation time is March 21, 2024, the preservation unit is China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.30076. The address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bifidobacterium lactis BL-PG-09 disclosed by the invention is separated from faeces of healthy infants, has the function of relieving mouse diarrhea caused by antibiotics, has a certain antibacterial effect on pathogenic bacteria such as staphylococcus aureus, bacillus subtilis and escherichia coli, and has relatively strong acid resistance, salt resistance, cholate resistance and artificial simulated gastrointestinal fluid tolerance.

The invention provides a mixed fermentation method for producing salidroside from escherichia coli and reducing metabolic accumulation of by-product acetic acid, and belongs to the technical field of microbial fermentation. The problem that the growth and metabolism of escherichia coli are inhibited due to accumulation of acetic acid byproducts in the escherichia coli liquid fermentation process is solved. The method comprises engineering escherichia coli with high yield of salidroside, bacillus megaterium as an auxiliary fermentation strain and a mixed fermentation method. The method is mainly used for reducing the metabolic accumulation of the by-product acetic acid in the fermentation process and eliminating the inhibition influence of the acetic acid on the growth and metabolism of escherichia coli through the method and the used strain, and finally the metabolic accumulation of the salidroside is maximized.

The invention provides a lactobacillus plantarum antibacterial peptide and application thereof in antibiosis, namely the antibacterial peptide separated from lactobacillus plantarum FJS003, and the antibacterial peptide is good in antibacterial stability and has a strong inhibition effect on the growth of staphylococcus aureus, escherichia coli, bacillus subtilis, pseudomonas aeruginosa, listeria monocytogenes and saccharomyces cerevisiae. The amino acid sequence of the provided antibacterial peptide is SEQ ID NO: 1. The antibacterial peptide provided by the invention can effectively inhibit the growth of staphylococcus aureus, escherichia coli, bacillus subtilis, pseudomonas aeruginosa, listeria monocytogenes and saccharomyces cerevisiae, has obvious beneficial effects, and can be widely applied to food processing.

The invention relates to bacillus enteritidis E7 as well as a biological preparation and application thereof. The bacillus haynesii is preserved in the China Center for Type Culture Collection on July 28, 2025, and the preservation number of the bacillus haynesii is CCTCC (China Center for Type Culture Collection) M 20251704. According to the invention, a strain of bacillus enteritidis E7 is separated from corn rhizosphere soil and is comprehensively identified, and the bacillus enteritidis E7 is identified to be bacillus enteritidis. Experiments prove that the bacillus innamolii E7 has nitrogen fixation capacity, after the strain is fermented, the activity of nitrogenase is 1752.5 mu mol / mL / h, absorption of corn plants to nitrogen elements can be promoted, and dependence on exogenous chemical nitrogen fertilizer is reduced; plant growth is promoted; fusarium graminearum and colletotrichum gloeosporioides can be inhibited, and the bacteriostasis rate is greater than 60%, so that various plant fungal diseases are prevented and treated. The invention has important application value in agricultural production.

The present application relates to the technical field of livestock and poultry breeding, and particularly relates to a compound microbial agent for improving the disease resistance of livestock and poultry and a preparation method thereof. The present application provides a microbial combination comprising Bacillus subtilis, Bacillus subtilis, Bacillus amyloliquefaciens, Yarrowia lipolytica and Trichoderma harzianum, and a compound microbial agent, which can effectively improve the disease resistance of livestock and poultry. In the specific embodiments of the present application, the survival rate of chicks infected with Escherichia coli can be significantly improved after treatment with the microbial combination provided by the present application, and the survival rate can reach more than 96%, and the body weight, chicken bursa index, spleen index and thymus index can be effectively recovered. Therefore, the microbial combination and the compound microbial agent provided by the present application can be used to improve the disease resistance of livestock and poultry, and provide a new idea for preparing products for improving the disease resistance of livestock and poultry.