35 results about "Bacillus enteritidis" patented technology

The invention discloses a novel biosynthesis path for producing 3 '-phosphoadenosine-5'-phosphosulfuric acid and application, and belongs to the technical field of bioengineering. According to the invention, through recombinant expression and purification of ribose kinase, ribose phosphate pyrophosphate kinase, ribose phosphate transferase, adenine thiotransferase, adenosine phosphate sulfate kinase and polyphosphate kinase 2, a six-enzyme cascade reaction taking ribose and adenosine as substrates in vitro is constructed, the molar conversion rate reaches 22.85%, and the escherichia coli and bacillus subtilis PAPS cell factory can be used for preparing the recombinant ribose phosphate pyrophosphate kinase, the ribose phosphate transferase, the adenine thiotransferase, the adenosine phosphate sulfate kinase and the polyphosphate kinase 2. The yield of PAPS in a 5-L fermentation tank reaches 7.60 g / L and 5.03 g / L respectively. The PAPS synthesis route provided by the invention provides an extensible, economical and efficient solution for the production of sulfated compounds.

The invention discloses bacillus amyloliquefaciens with the characteristic of inhibiting mould growth and application of the bacillus amyloliquefaciens, and belongs to the field of microorganisms and fermentation engineering, the bacillus amyloliquefaciens is preserved in China General Microbiological Culture Collection Center on August 5, 2024, and the preservation number is CGMCC No.31538. The strain is bacillus amyloliquefaciens obtained by screening, separating and purifying from peasant pickles, and the strain can inhibit the growth of escherichia coli, clostridium perfringens and mould. The bacillus amyloliquefaciens disclosed by the invention solves the problem of insufficient effect of a microorganism mildew-proof method of the existing fermented feed, and the bacillus amyloliquefaciens is added into the biological fermented feed, so that the quantity of harmful microorganisms in the biological fermented feed can be effectively reduced, the generation of mycotoxin is inhibited, the feed is prevented from mildewing and going bad, and the shelf life of the feed is prolonged.

The invention provides bacillus velezensis SFT4 with an antibacterial function and application thereof, and belongs to the technical field of microbe application, the bacillus velezensis SFT4 has a good inhibition effect on Erpex lacteus, Aspergillus pallidum, Clostridium perfringens, Staphylococcus aureus, Micrococcus luteus and the like, has a certain inhibition effect on salmonella, and can be used for preparing the bacillus velezensis SFT4. The invention provides a bacillus velezensis SFT4 and a metabolite thereof, provides a microecological preparation containing the bacillus velezensis SFT4 and the metabolite thereof, and provides a use method of the microecological preparation, the microecological preparation is composed of the bacillus velezensis SFT4, lactobacillus reuteri, lactobacillus casei and lactobacillus plantarum, and the microecological preparation is applied to the livestock and poultry industry. The microecological preparation can effectively prevent and treat clostridium perfringens and escherichia coli, can be prepared into a feed additive for use, can be directly fed through a drinking water line, and is not easy to block the drinking water feeding line.

The application discloses an Escherichia coli probiotic with high beta-alanine yield and application thereof, and the recombinant genetically engineered bacterium takes a wild-type Escherichia coli probiotic as a starting strain and is subjected to one or more gene edits, including Trc promoter strengthening of a panD gene, an aspC gene, an sdhC gene and a ppc gene, overexpression of a panD gene from Bacillus subtilis, an aspB gene from Corynebacterium glutamicum, an aspA gene and a pyc gene from Corynebacterium glutamicum on a plasmid pTrc99a, and knockout of a cycA gene. 600 Compared with an existing reported Escherichia coli probiotic beta-alanine production strain, the OD of the recombinant genetically engineered bacterium is 4.4 times that of the existing reported strain, and the beta-alanine yield is 6 times that of the existing reported strain, so that the recombinant genetically engineered bacterium has great industrial application potential.

The invention discloses an escherichia coli-bacillus subtilis shuttle plasmid and application, and belongs to the field of gene engineering. The shuttle plasmid comprises the following elements: a promoter sequence, a coding sequence of bacillus subtilis signal peptide, a replication initiation sequence of bacillus subtilis, a replication initiation sequence of escherichia coli and an antibiotic resistance gene. According to the plasmid design, a modular structure is adopted, and quick replacement of key elements such as a promoter, a signal peptide and RBS is supported through optimized enzyme cutting site layout; a 6 * His tag introduced at the tail end of a multiple cloning site (MCS) can be adapted to nickel column affinity chromatography, and the purification process of the recombinant protein is simplified. The plasmid has the characteristics of small molecular weight and convenience in operation, can be efficiently cloned and constructed in escherichia coli and then directly converted into bacillus subtilis for protein expression, is particularly suitable for development of a gram-positive bacterium secretory expression system, and provides an efficient tool for large-scale production of industrial enzyme preparations.

The invention discloses Escherichia coli probiotics with high yield of beta-alanine and application of the Escherichia coli probiotics. The recombinant genetically engineered bacterium takes wild type Escherichia coli probiotics as a starting strain, and edits one or more of the following genes: a Trc promoter enhanced panD gene, an aspC gene, an sdhC gene and a ppc gene; a panD gene from bacillus subtilis, an aspB gene and an aspA gene from corynebacterium glutamicum and a pyc gene from corynebacterium glutamicum are over-expressed on a plasmid pTrc99a, and a cycA gene is knocked out. Compared with the existing reported Escherichia coli probiotic beta-alanine production strain, the OD600 of the recombinant genetic engineering strain is 4.4 times of that of the existing reported strain, the yield of beta-alanine is 6 times of that of the existing reported strain, and the recombinant genetic engineering strain has huge industrial application potential.

The invention provides a molybdenum disulfide nano material rich in sulfur defects and a preparation method and application thereof, and belongs to the technical field of nano material preparation and sterilization. Starting from the properties of the molybdenum disulfide nano-material, sulfur defects with different concentrations are introduced to the surface of the nano-material through high-temperature calcination, and the molybdenum disulfide nano-material rich in sulfur defects is prepared; the molybdenum disulfide nano material rich in sulfur defects is in the shape of nano flower balls with the diameter of 200-300 nm, has the optimal photothermal conversion efficiency and high adsorbability under near-infrared light irradiation, and can efficiently inhibit the activity of escherichia coli and bacillus subtilis; the preparation method disclosed by the invention is simple in process, stable in reaction system, wide in reactant source and environment-friendly, shows a universal sterilization effect, is expected to introduce a new method in the field of sterilization of nano materials, provides a new technology and a new idea for design and preparation of functional nano materials, and has very good practicability.

The present application relates to the field of pharmaceutical chemistry, and specifically discloses a emodin mannich base, a preparation method and application thereof. The emodin mannich base has a structure as shown in formula I, the structure is simple, has strong in-vitro antimicrobial activity, especially high inhibitory activity to staphylococcus aureus, bacillus subtilis, micrococcus luteus, escherichia coli, proteus vulgaris, pseudomonas aeruginosa, shigella dysenteriae, salmonella typhimurium and other bacteria, and candida utilis, candida albicans, saccharomyces cerevisiae, monascus and other fungi, and can be used for preparing antibacterial and / or antifungal drugs.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

A modified Escherichia coli and the use thereof in the production of L-amino acid by means of fermentation. Specifically, disclosed is the use of pyruvate formate lyase, alcohol dehydrogenase, branched-chain amino acid transaminase derived from Escherichia coli, branched-chain amino acid transaminase derived from Bacillus subtilis, thiamine phosphate synthase and malate dehydrogenase in the construction of a genetically engineered bacterium for producing L-amino acid. Experiments prove that the recombinant bacterium, which is conducive to the accumulation of L-amino acid, is obtained by means of knocking out the encoding genes of the pyruvate formate lyase, the alcohol dehydrogenase, the branched-chain amino acid transaminase derived from Escherichia coli, the thiamine phosphate synthase and the malate dehydrogenase of Escherichia coli capable of producing L-amino acid, and introducing the encoding gene of the branched-chain amino acid transaminase derived from Bacillus subtilis. The recombinant bacteria can greatly increase the yield of L-amino acid and reduce the costs, and is of great significance to accelerate the industrial progress of L-amino acid.

This invention discloses the antimicrobial peptide PJX13 and its application in the control of citrus canker. The sequence of the antimicrobial peptide PJX13 is AcKKFWKLRRWVKWIGNH2. It can effectively inhibit and kill *Xanthomonas citrus* by disrupting the cell membrane integrity of pathogens, leading to leakage of intracellular substances. It effectively inhibits the expansion of lesions on both detached citrus leaves and living plants, and can be formulated into an antimicrobial product against *Xanthomonas citrus* for the control of citrus canker. Furthermore, the antimicrobial peptide PJX13 can also inhibit *Escherichia coli*, *Staphylococcus aureus*, and *Bacillus oryzae*, and can be used to prepare broad-spectrum antimicrobial products. This invention is beneficial for the control of citrus canker, and has advantages such as being environmentally friendly and unlikely to induce drug resistance.

The invention relates to the technical field of livestock and poultry breeding, in particular to a complex microbial inoculant for improving disease resistance of livestock and poultry and a preparation method of the complex microbial inoculant. The invention provides a microbial composition containing bacillus siamensis, bacillus subtilis, bacillus amyloliquefaciens, yarrowia lipolytica and trichoderma harzianum and a complex microbial inoculant. The microbial composition and the complex microbial inoculant can effectively improve the disease resistance of livestock and poultry. In the specific embodiment of the invention, after the microbial composition provided by the invention is applied to treatment, the survival rate of chicks infected with escherichia coli can be remarkably increased, the survival rate can reach 96% or above, and the weight, the chicken bursa of Fabricius index, the spleen index and the thymus index can be effectively recovered. Therefore, the microbial composition and the complex microbial inoculant provided by the invention can be used for improving the disease resistance of the livestock and poultry, and a new idea is provided for preparing products for improving the disease resistance of the livestock and poultry.

The application discloses a composite biological deodorant, which comprises the following components in parts by weight: 1-3 parts of Bacillus coagulans slurry; 1-3 parts of Saccharomyces cerevisiae slurry; 1-3 parts of Lactobacillus plantarum slurry; 10-30 parts of nutrient solution A; and 20-60 parts of nutrient solution B. The composite biological deodorant is prepared by using the composite system of the Bacillus coagulans slurry, the Saccharomyces cerevisiae slurry and the Lactobacillus plantarum slurry, and combining with a specific nutrient solution carrier. The Bacillus coagulans provides environmental tolerance guarantee for the composite system due to strong environmental resistance. Metabolic products secreted by the Saccharomyces cerevisiae help to promote the proliferation of the Lactobacillus plantarum. Bacteriocins produced by the Lactobacillus plantarum selectively inhibit staphylococcus aureus, escherichia coli and other foul-smelling pathogenic bacteria, and degrade small-molecule foul-smelling substances such as ammonia nitrogen and hydrogen sulfide. The three components are synergistic, so that the deodorization and bacteriostasis effects are achieved. The product has no strong peculiar smell, and the risk of contamination by miscellaneous bacteria is effectively avoided.

The present invention discloses a strain of Bacillus Velez CB6 and the antimicrobial protein produced and its application, belonging to the field of microbial application technology. The deposit number of the Bacillus Velez CB6 is CGMCC NO.30515. The strain has a good antagonistic effect on Gram-negative bacteria such as multidrug-resistant Escherichia coli. The antimicrobial protein CB6-E prepared by fermentation of the strain shows good antimicrobial activity against a variety of Gram-negative bacteria, and has good safety for a variety of animals. CB6-E has a good therapeutic effect on mice and wax moths infected with polymyxin-resistant and carbapenem-resistant Escherichia coli. The antimicrobial protein CB6-E prepared by fermentation of Bacillus Velez CB6 of the present invention is expected to enrich the current existing antimicrobial drug library and provide new hope for the treatment of multidrug-resistant Gram-negative bacterial infections.

The invention discloses an antibacterial peptide NyAmp-a and application thereof, and relates to the technical field of biological medicines. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID NO. 1. The invention also provides an application of the antibacterial peptide NyAmp-a in preparation of an antibacterial product for inhibiting staphylococcus aureus, bacillus subtilis, escherichia coli and aeromonas hydrophila, and a minimum inhibitory concentration of the antibacterial peptide NyAmp-a to each bacterium. The invention provides a novel efficient antibacterial solution for four common pathogenic bacteria, namely staphylococcus aureus, bacillus subtilis, escherichia coli and aeromonas hydrophila. The antibacterial peptide NyAmp-a has the characteristic that drug resistance is not easy to generate, a related antibacterial composition can be applied to the fields of medicine, agriculture, food and the like, has important practical application value, and effectively solves the problems that traditional antibiotics are easy to generate drug resistance, and existing antibacterial peptides are high in cytotoxicity and lack of selectivity for specific strains.

The present invention discloses a homologous recombinant Bacillus subtilis expressing cecropin B gene and a construction method thereof, and belongs to the field of genetic engineering technology. The present invention discloses a homologous recombinant Bacillus subtilis expressing cecropin B gene and a construction method thereof, using Bacillus subtilis 1A1 as a starting strain, knocking out the coding region of the endo-1,4-β-xylanase gene, and integrating the expression of the cecropin B gene, and using xylan induction to obtain the Bacillus subtilis genetic engineering bacteria expressing the cecropin B gene and having the antibacterial activity of inhibiting Salmonella, Escherichia coli and Listeria monocytogenes. The recombinant bacteria can be used as an animal health product for preventing and treating bacterial diseases of the digestive tract of livestock, poultry and aquatic animals.

The invention discloses bacillus velezensis LYBV022 and application thereof in preparation of a preparation for inhibiting clostridium perfringens infection, and belongs to the technical field of microorganisms. The invention discloses a bacillus velezensis LYBV022, and the preservation number of the bacillus velezensis LYBV022 is GDMCC No: 66952. The bacillus velezensis LYBV022 disclosed by the invention has a relatively good antibacterial ability on clostridium perfringens, staphylococcus aureus, escherichia coli and the like, so that the bacillus velezensis LYBV022 has a clinical application potential.

The present invention discloses a teratocyte serine protease inhibitor of Cottonella plutellae and its application. The present invention clones a CvT-SPN-bacteriostat gene, a serine protease inhibitor of Cottonella plutellae. The recombinant protein obtained by in vitro prokaryotic expression of the CvT-SPN-bacteriostat gene can effectively inhibit the growth of pathogenic bacteria Escherichia coli and Staphylococcus aureus of its host, Plutella xylostella, thereby avoiding the impact of bacterial infection of the host on the parasitic wasp larvae, which is beneficial to the growth and development of the parasitic wasp larvae in the diamondback moth. It provides a reference for improving the biological control efficiency of parasitic wasps and also provides potential possibilities for the research and development of human antibacterial drugs and food antibacterial agents. At the same time, the recombinant protein can inhibit the activity of Bacillus subtilis protease and trypsin, which has important reference value for the development of new serine protease inhibitor drugs.

The invention discloses a composite biological deodorant which comprises the following components in parts by weight: 1-3 parts of bacillus coagulans bacterial sludge; 1-3 parts of saccharomyces cerevisiae mud; 1-3 parts of plant lactobacillus bacterial sludge; 10-30 parts of a nutrient solution A; and 20-60 parts of a nutrient solution B. The composite biological deodorant is prepared by innovatively adopting a compound system of bacillus coagulans bacterial sludge, saccharomyces cerevisiae bacterial sludge and plant lactobacillus bacterial sludge and combining with a specific nutrient solution carrier, and bacillus coagulans provides environmental tolerance guarantee for a compound system by virtue of strong environmental resistance; metabolites secreted by the saccharomyces cerevisiae are beneficial to promoting proliferation of the plant lactobacillus, and bacteriocin generated by the plant lactobacillus selectively inhibits staphylococcus aureus, escherichia coli and other malodorous pathogenic bacteria and degrades ammonia nitrogen, hydrogen sulfide and other micromolecular malodorous substances at the same time; the three components have a synergistic effect, so that a remarkable deodorization and bacteriostasis effect is achieved, the product is free of strong peculiar smell, and the risk of infectious microbe pollution is effectively avoided.

The invention belongs to the technical field of chemical synthesis of medicines, and particularly discloses a 1, 4-benzodioxane thiourea compound as well as a preparation method and application thereof, the structural general formula of the 1, 4-benzodioxane thiourea compound is as follows: in the formula, R group is methyl, methoxyl or halogen group. The 1, 4-benzodioxane thiourea compound has an inhibiting effect on at least one of staphylococcus aureus, bacillus subtilis, escherichia coli and pseudomonas aeruginosa, the synthetic route is simple, the used raw materials are cheap and easy to obtain, the yield is high, and the 1, 4-benzodioxane thiourea compound is suitable for industrial production and is an antibacterial drug with huge potential.

The present invention discloses a soap with mite removal and antibacterial efficacy and a preparation method thereof. The soap comprises the following components by weight: 5-10 parts of a Sargassum fusiformis extract, 3-8 parts of a Magnolia bark extract, 1-5 parts of a Paeonia suffruticosa root bark extract, 2-6 parts of a Sanguisorba officinalis extract, 20-30 parts of olive oil, 10-20 parts of sodium hydroxide, and 15-35 parts of water. By compounding a specific ratio of the Sargassum fusiformis extract, the Magnolia bark extract, the Paeonia suffruticosa root bark extract, and the Sanguisorba officinalis extract, the soap has a good inhibitory effect on Staphylococcus aureus, Escherichia coli, and Bacillus subtilis, thereby reducing bacterial damage to the human body. The soap is supplemented with polyglutamic acid with a relative molecular weight of 400,000-700,000 to improve the problem of the soap being less durable and cracking due to the addition of glycerin.

The application belongs to the technical field of industrial circulating water treatment, and provides a green water treatment agent with corrosion inhibition and bacteriostasis performance, a preparation method and application, the water treatment agent has the structure as shown: The application introduces a piperazine group into a polyaspartic acid peptide bond structure by a graft copolymerization method, synthesizes a novel water treatment agent, the water treatment agent can be adsorbed on a metal surface to form a protective film, has good corrosion inhibition and bacteriostasis performance. The water treatment agent also has bacteriostasis specificity, can inhibit escherichia coli, and retains bacillus cereus. Therefore, the application realizes efficient improvement of the corrosion inhibition performance and bacteriostasis performance of polyaspartic acid, reduces the application of phosphorus-containing compounds, and avoids the influence on the environment.

The present invention belongs to the technical field of molecular biology and relates to a recombinant protein rCgDM9CP-7 from the Pacific oyster Crassostrea gigas containing a DM9 domain and its application. The amino acid sequence of the recombinant protein rCgDM9CP-7 from the Pacific oyster Crassostrea gigas containing a DM9 domain is shown in SEQ ID NO.1; an amino acid sequence having a homology of greater than or equal to 90% thereto. The application of the recombinant protein in preparing an agglutination preparation for Vibrio splendidus, Escherichia coli, Staphylococcus aureus or Bacillus subtilis to inhibit the growth of the strains.

The application discloses mulberry leaf volatile oil and a preparation method and application thereof, and relates to the technical field of plant extraction. The preparation method of the mulberry leaf volatile oil comprises the following steps: (1) crushing mulberry leaves to obtain mulberry leaf powder; (2) mixing the mulberry leaf powder with deionized water, and then using a microwave-assisted hydro-distillation (MAHD) method to obtain a distillate; and (3) using an organic solvent to extract the distillate to obtain the mulberry leaf volatile oil. The mulberry leaf volatile oil is extracted from mulberry leaves by using the MAHD method, the extraction process is optimized through single-factor experiments and a response surface design, and the optimal extraction process is obtained. It is verified that the mulberry leaf volatile oil prepared by the application has antibacterial activity on bacillus subtilis, salmonella paratyphi B, pseudomonas aeruginosa, escherichia coli and staphylococcus aureus, and has a strong scavenging capacity on ABTS and DPPH free radicals.

The present invention relates to the technical field of non-antibiotic prevention and control in the aquaculture industry, and in particular to a composition containing a perilla extract, and preparation and application thereof. The composition containing the perilla extract comprises raw materials in the following mass ratio: 0.8-1.2 parts of a mixed powder, 20-30 parts of water, and 0.022-0.033 parts of a fermentation bacterium; the mixed powder is prepared by mixing perilla leaf powder and osmanthus chinensis powder; the fermentation bacterium is a composite strain obtained by mixing Bacillus coagulans and Lactobacillus fermentation in a mass ratio of 0.5-5:1, or a single strain of either Bacillus coagulans or Lactobacillus fermentation. The present invention treats chickens with Escherichia coli model disease by combining perilla leaves and osmanthus chinensis, then fermenting Bacillus coagulans and Lactobacillus fermentation for a certain period of time, and then preparing a fermented product by a specific fermentation technology. The method solves a series of problems in chickens infected with Escherichia coli, such as high mortality, reduced daily weight gain, increased feed-to-meat ratio, immune damage, and intestinal damage, and finds that the composition prepared under appropriate conditions has better effects.

The invention relates to the field of medicinal chemistry, and particularly discloses an emodin Mannich base as well as a preparation method and application thereof. The emodin Mannich base has a structure as shown in a formula I. The emodin Mannich base is simple in structure and has relatively strong in-vitro antimicrobial activity; the compound has very high inhibitory activity on bacteria such as staphylococcus aureus, bacillus subtilis, micrococcus luteus, escherichia coli, proteusbacillus vulgaris, pseudomonas aeruginosa, shigella dysenteriae and salmonella typhimurium, and fungi such as candida utilis, candida albicans, saccharomyces cerevisiae and monascus purpureus. The compound can be used for preparing antibacterial and / or antifungal drugs. # imgabs0 #

The utility model discloses a bacillus / escherichia coli engineering strain efficient screening device which comprises a deep hole plate, hole grooves are evenly formed in the deep hole plate, screening holes are evenly formed in the bottom walls of the hole grooves, a bottom plate is arranged below the deep hole plate, containing grooves are evenly formed in the bottom plate, and the containing grooves correspond to the hole grooves one to one. The containing groove is used for containing filtered substances in the hole groove, a clamping fixing assembly is installed between the deep hole plate and the hole groove, positioning grooves are formed in the four corners of the top end of the bottom plate, and positioning blocks are installed at the four corners of the bottom end of the deep hole plate; in the working process, when the deep hole plate is installed above the bottom plate, the inserting plate is inserted into the inserting groove, the deep hole plate and the bottom plate are clamped and fixed, meanwhile, under the limiting effect of the inserting plate, the bottom wall of the deep hole plate is tightly attached to the top wall of the bottom plate, and the hole grooves correspond to the containing grooves one to one; the solution is prevented from entering other containing grooves through the gap between the deep hole plate and the bottom plate, and the screening effect is improved.

The invention belongs to the technical field of microorganisms, and relates to a bifidobacterium lactis BL-PG-09 strain capable of relieving diarrhea and a preparation method of the bifidobacterium lactis BL-PG-09 strain, the strain is bifidobacterium lactis BL-PG-09, the preservation number is CGMCC No.30076, the preservation time is March 21, 2024, the preservation unit is China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.30076. The address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bifidobacterium lactis BL-PG-09 disclosed by the invention is separated from faeces of healthy infants, has the function of relieving mouse diarrhea caused by antibiotics, has a certain antibacterial effect on pathogenic bacteria such as staphylococcus aureus, bacillus subtilis and escherichia coli, and has relatively strong acid resistance, salt resistance, cholate resistance and artificial simulated gastrointestinal fluid tolerance.

The present invention provides a Bacillus velezensis SFT4 with antibacterial function and its application, belonging to the technical field of microbial application. Bacillus velezensis SFT4 has good inhibitory effects on Irpex lacteus, Aspergillus pallidus, Clostridium perfringens, Staphylococcus aureus, Micrococcus luteus, etc., and has a certain inhibitory effect on Salmonella. A probiotic preparation containing Bacillus velezensis SFT4 and its metabolites is provided, and a method for using the probiotic preparation is provided. The probiotic preparation is composed of Bacillus velezensis SFT4, Lactobacillus reuteri, Lactobacillus casei, and Lactobacillus plantarum. The application of the probiotic preparation in the livestock and poultry breeding industry can effectively prevent and control Clostridium perfringens and Escherichia coli, and the probiotic preparation can be made into a feed additive or directly fed through a drinking water line, and it is not easy to block the drinking and feeding pipeline.

The invention provides a mixed fermentation method for producing salidroside from escherichia coli and reducing metabolic accumulation of by-product acetic acid, and belongs to the technical field of microbial fermentation. The problem that the growth and metabolism of escherichia coli are inhibited due to accumulation of acetic acid byproducts in the escherichia coli liquid fermentation process is solved. The method comprises engineering escherichia coli with high yield of salidroside, bacillus megaterium as an auxiliary fermentation strain and a mixed fermentation method. The method is mainly used for reducing the metabolic accumulation of the by-product acetic acid in the fermentation process and eliminating the inhibition influence of the acetic acid on the growth and metabolism of escherichia coli through the method and the used strain, and finally the metabolic accumulation of the salidroside is maximized.