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Enteric bacilli with fixing effect on cadmium capable of promoting plant growth and application of enteric bacilli

A technology that promotes plant growth and has a fixed effect. It is applied in the direction of plant growth regulators, plant growth regulators, applications, etc., to repair cadmium-contaminated soil, reduce content, and promote plant growth.

Active Publication Date: 2015-06-03
SICHUAN LANYUE SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in practical applications, engineering treatment measures are still only palliatives but not the root cause in the case of spending a lot of manpower and money; physical and chemical remediation methods are only suitable for small-scale pollution remediation; chemical remediation through the adsorption of heavy metals, redox, antagonistic or precipitation Reducing heavy metal pollution is a widely used method to control heavy metal pollution, but there may also be a risk of secondary pollution from chemical substances

Method used

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  • Enteric bacilli with fixing effect on cadmium capable of promoting plant growth and application of enteric bacilli
  • Enteric bacilli with fixing effect on cadmium capable of promoting plant growth and application of enteric bacilli
  • Enteric bacilli with fixing effect on cadmium capable of promoting plant growth and application of enteric bacilli

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (3) Streak separation: Pick different colonies of cadmium-resistant bacteria on the 9 plates obtained in step (2) and culture them by streaking on solid nitrogen-improved basal medium plates until pure colonies are isolated. The isolated pure strains were inoculated on the slant medium, and after the bacteria grew out, they were placed in a refrigerator at 4°C for use. The composition of the slant medium is the same as that of the solid nitrogen-improved basal medium.

[0026] (4) Screening of IAA-secreting strains: Inoculate the cadmium-resistant strains obtained in step (3) into Erlenmeyer flasks with 50ml of liquid R2A medium, and culture them on a shaker at 28°C with a rotation speed of 150rpm. After 4 days, 50 μL of its bacterial suspension was dropped on a white ceramic plate, and 50 μL of colorimetric solution (Salkowski colorimetric solution: 50mL35%HClO4+1mL0.5mol / LFeCl3) was added to the bacterial suspension. The white ceramic plate was placed at room tempera...

Embodiment 2

[0038] from figure 1 It can be seen that after 45-70 hours of treatment, the removal rate of cadmium can reach 70-75%, and the removal effect is obvious. In practical application, the bacterial strain provided by the present invention is used to inoculate the cadmium-contaminated water body, and the clear water after the bacterial strain grows and reproduces, adsorbs the cadmium and precipitates is the purified water body.

[0039] Example 3:

[0040] State change of cadmium in soil of Enterobacter LY6

[0041] (1) Inoculate a loop of Enterobacter LY6 into 50ml of nitrogenous basal medium from a slant preservation tube, place it in a shaker at 28°C at 150rpm / min and shake it to the exponential phase, and use it as the seed liquid; take the seed liquid to inoculate with 1% volume Inoculate an appropriate amount into 0.5L of fresh medium, place in a shaker at 28°C at 150rpm / min and shake until the index is ready for use. The medium formula is as follows: sucrose, 10g; K 2 H...

Embodiment 3

[0044] from figure 2 and image 3 It can be seen from the comparison that after 28 days (4 weeks) of strain propagation by inoculating Enterobacter LY6, the content of exchangeable cadmium in the soil was significantly reduced. 50% of cadmium is adsorbed and fixed in inactive forms such as carbonate-bound state, iron-manganese oxide-bound state, and organic matter-bound state, which greatly reduces the content of cadmium in the exchangeable state and other effective states in the soil, and reduces the impact of cadmium on plants. pollute.

[0045] Example 4:

[0046] Effect of Enterobacter LY6 Secreting Auxin IAA

[0047] (1) Inoculate a loop of Enterobacter LY6 into 50ml medium from a slant preservation tube, place it in a shaker at 28°C at 150rpm / min and shake it to the exponential phase, and use it as the seed solution; take the seed solution and inoculate it with an inoculum volume of 1% to In 0.5L of fresh culture medium, place in a shaker at 28°C at 150rpm / min for s...

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Abstract

The invention discloses enteric bacilli with a fixing effect on cadmium capable of promoting plant growth and application of the enteric bacilli, and aims at solving the problem of heavy metal cadmium pollution and the problem that plants are difficult to grow in cadmium-polluted soil. The Latin name of the enteric bacilli is eterobacter sp; the preservation unit is the China General Microbiological Culture Collection Center; the preservation date is August 25, 2014; and the preservation number is CGMCC No.9539. Cadmium in a water-polluted environment can be gathered by the adsorption effect of a bacterial strain on the cadmium, so as to be removed; and the target of purifying a water body is achieved. Meanwhile, the bacterial strain is capable of adsorbing and fixing the heavy metal cadmium in soil, and reducing the content of effective cadmium in soil. In addition, the bacterial strain has the effect of synthesizing and secreting auxin IAA, is capable of accelerating rapid growth of plants, and has wide application prospect in practical production.

Description

Technical field: [0001] The invention relates to microorganisms screened in soil, in particular to bacteria enterobacterium with high performance of fixing cadmium and its application in bioremediation of water and soil polluted by heavy metal cadmium. Background technique: [0002] Heavy metals, as a class of harmful pollutants, have the characteristics of mobility, long cycle, easy accumulation and high toxicity. The discharge of pollutants and the application of pesticides and fertilizers enter the soil and water bodies, and soil and water are resources that human beings depend on for survival. Therefore, how to prevent and reduce heavy metals from endangering human health through soil, water sources, food chains, etc. has attracted widespread attention from society and academic circles. [0003] Cadmium (Cd) is a rare and dispersed heavy metal with a low background value in nature and is widely used in human activities in industry, agricultural production and other fie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A01P21/00B09C1/10C12R1/01C02F101/22
CPCA01N63/00B09C1/10C02F3/34C02F2101/22C12N1/205C12R2001/01
Inventor 侯勇张亮夏中梅王强峰朱彭玲黄秀辉胡苏王赟曾芸王兰英曾显斌
Owner SICHUAN LANYUE SCI & TECH
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