Bacillus velezensis strain wr8 and application thereof
A technology of Bacillus and Veles, applied in the field of microorganisms, to achieve a wide range of effects in the field of application
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Embodiment 1
[0045] Screening of strain Bacillus velezensis wr8:
[0046]The Bacillus velezensis wr8 strain, whose preservation number is GDMCC NO.61655, is preserved in: Guangdong Microbial Culture Collection Center, address: 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou, and the preservation date is 2021 May 11.
[0047] The above-mentioned strains were collected from the epidermis of Wogan fruit in Wuming, Guangxi in 2021.
[0048] Carry out morphological classification and molecular biology identification to the microorganism of present embodiment specifically as follows:
[0049] 1. Morphological classification of strains
[0050] Morphological classification of strains:
[0051] The bacterial strain wr8 was inoculated on the LB medium, and the morphological characteristics of the colonies were observed, such as figure 1 As shown, the colony is white on the plate, the edges of the colony are not smooth, there are folds in the middle, and the single colon...
Embodiment 2
[0055] This example mainly studies the confrontation effect of strain wr8 on different microorganisms.
[0056] 1. Plate confrontation experiment against mold:
[0057] 1. The confrontation effect on Botrytis cinerea:
[0058] ①Preparation of pathogenic bacteria suspension: add the agar medium with pathogenic bacteria to the PDB liquid medium, culture at 28°C and 170rpm for 7 days, filter the suspension through four layers of gauze after the cultivation, and then adjust the final concentration with distilled water to 10×10 6 cfu / ml, spare.
[0059] ② Inoculate the suspension of the above-mentioned pathogenic bacteria on the PDA medium, in which the control group is only inoculated with the pathogenic bacteria of the new strain of the application; while the experimental group is inoculated with the pathogenic bacteria on one side of the PDA medium, and the corresponding screening strain is inoculated on the opposite side : wr8 for antagonistic experiments.
[0060] Tablet c...
Embodiment 3
[0102] The present embodiment mainly studies the inhibitory effect of bacterial strain wr8 on citrus plant mold, specifically as follows:
[0103] 1. Inhibition experiment on Botrytis cinerea:
[0104] Use the inoculation needle to pick the fungal hyphae and inoculate the epidermis of the oranges, and inoculate 50 oranges altogether, wherein 25 are the experimental group and 25 are the control group. The control group and the experimental group are respectively processed as follows:
[0105] Experimental group: Spray the bacterial suspension containing the strain wr8 on the orange skin, repeat the drip application every 72 hours, the drip application amount is 10ul, and the number of viable bacteria in the bacterial suspension is 1×10 6 cfu / ml.
[0106] Control group: drip distilled water to the orange skin, repeat the drip application every 72 hours, and the drip application amount is 10ul.
[0107] Cultivate the above-mentioned oranges at room temperature, and observe the ...
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