Bacillus velezensis strain wr8 and application thereof

A technology of Bacillus and Veles, applied in the field of microorganisms, to achieve a wide range of effects in the field of application

Active Publication Date: 2021-12-31
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The application of microorganisms in the fields of biological pesticides, biological fertilizers, and biological fresh-keeping has a long history. In actual work, those skilled in the art often screen microorganisms that have an inhibitory effect on mold and pathogenic bacteria as bio-control bacteria. Because microorganisms have green , environmental protection, not easy to cause chemical residues, environmental pollution and other problems, it has been widely promoted and applied at present; however, the biggest bottleneck in the application of bio-control bacteria is: how to enrich the microbial germplasm resource bank, and screen more bacteria with good disease resistance and antibacterial Sexual strains key to development in the field
In the long-term study, the applicant found that Bacillus velezensis (Bacillus velezensis) is a class of microorganisms with good antibacterial and biocontrol effects. application, the applicant has also been committed to the development and research of various plant biocontrol bacteria, but in actual work, the applicant found that: different strains belonging to the same species have relatively large differences in their physiology, and some strains can inhibit certain The growth of a pathogenic bacterium, but other strains of the same genus do not necessarily have the same antibacterial effect as it. Therefore, the screening and research and development of strains is a never-ending research field. The discovery of new strains and applications of new strains It will provide a solid foundation for subsequent plant protection, food preservation and processing, biological bacterial fertilizer and other related biological protection fields

Method used

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  • Bacillus velezensis strain wr8 and application thereof
  • Bacillus velezensis strain wr8 and application thereof
  • Bacillus velezensis strain wr8 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Screening of strain Bacillus velezensis wr8:

[0046]The Bacillus velezensis wr8 strain, whose preservation number is GDMCC NO.61655, is preserved in: Guangdong Microbial Culture Collection Center, address: 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou, and the preservation date is 2021 May 11.

[0047] The above-mentioned strains were collected from the epidermis of Wogan fruit in Wuming, Guangxi in 2021.

[0048] Carry out morphological classification and molecular biology identification to the microorganism of present embodiment specifically as follows:

[0049] 1. Morphological classification of strains

[0050] Morphological classification of strains:

[0051] The bacterial strain wr8 was inoculated on the LB medium, and the morphological characteristics of the colonies were observed, such as figure 1 As shown, the colony is white on the plate, the edges of the colony are not smooth, there are folds in the middle, and the single colon...

Embodiment 2

[0055] This example mainly studies the confrontation effect of strain wr8 on different microorganisms.

[0056] 1. Plate confrontation experiment against mold:

[0057] 1. The confrontation effect on Botrytis cinerea:

[0058] ①Preparation of pathogenic bacteria suspension: add the agar medium with pathogenic bacteria to the PDB liquid medium, culture at 28°C and 170rpm for 7 days, filter the suspension through four layers of gauze after the cultivation, and then adjust the final concentration with distilled water to 10×10 6 cfu / ml, spare.

[0059] ② Inoculate the suspension of the above-mentioned pathogenic bacteria on the PDA medium, in which the control group is only inoculated with the pathogenic bacteria of the new strain of the application; while the experimental group is inoculated with the pathogenic bacteria on one side of the PDA medium, and the corresponding screening strain is inoculated on the opposite side : wr8 for antagonistic experiments.

[0060] Tablet c...

Embodiment 3

[0102] The present embodiment mainly studies the inhibitory effect of bacterial strain wr8 on citrus plant mold, specifically as follows:

[0103] 1. Inhibition experiment on Botrytis cinerea:

[0104] Use the inoculation needle to pick the fungal hyphae and inoculate the epidermis of the oranges, and inoculate 50 oranges altogether, wherein 25 are the experimental group and 25 are the control group. The control group and the experimental group are respectively processed as follows:

[0105] Experimental group: Spray the bacterial suspension containing the strain wr8 on the orange skin, repeat the drip application every 72 hours, the drip application amount is 10ul, and the number of viable bacteria in the bacterial suspension is 1×10 6 cfu / ml.

[0106] Control group: drip distilled water to the orange skin, repeat the drip application every 72 hours, and the drip application amount is 10ul.

[0107] Cultivate the above-mentioned oranges at room temperature, and observe the ...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a bacillus velezensis strain wr8 and application thereof. The bacillus velezensis wr8, fungicide and metabolite have the wide antibacterial and disease-resistant effects, and have the inhibiting effect on penicillium, penicillium, botrytis cinerea, escherichia coli, listeria monocytogenes and other germs, and in the direct application, the strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, and can be used for preventing and treating banana fusarium wilt, orange mycosis, citrus reiculata mycosis and the like; the bacillus velezensis strain wr8 can be applied to fruit preservation of the plants, and the application range of the bacterial fertilizer biocontrol field is quite wide; and meanwhile, the strain can inhibit escherichia coli and listeria monocytogenes, can also be applied to the field of food preservation, has multiple functions and wide application fields, and has good prospects in the fields of later food processing, fruit and vegetable preservation, biological control and biological bacterial fertilizers.

Description

【Technical field】 [0001] The invention relates to the technical field of microbes, in particular to the bacillus velei strain wr8 and its application. 【Background technique】 [0002] The application of microorganisms in the fields of biological pesticides, biological fertilizers, and biological fresh-keeping has a long history. In actual work, those skilled in the art often screen microorganisms that have an inhibitory effect on mold and pathogenic bacteria as bio-control bacteria. Because microorganisms have green , environmental protection, not easy to cause chemical residues, environmental pollution and other problems, it has been widely promoted and applied at present; however, the biggest bottleneck in the application of bio-control bacteria is: how to enrich the microbial germplasm resource bank, and screen more bacteria with good disease resistance and antibacterial Sexual strains are key to the development of this field. In the long-term study, the applicant found t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00A23B7/155C05F11/08C05G3/60C12R1/07
CPCA01N63/22A23B7/155C05F11/08C05G3/60A23V2002/00A23V2200/10
Inventor 周礼芹王小虎王睿韦圣博朱绮霞叶柳健何双银梦贺瑜岚兰宝峰岑锦艺
Owner GUANGXI ACAD OF SCI
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