A biological composite preservative, its preparation method and its application in sturgeon surimi products
A composite fresh-keeping and biological technology, which is applied in the direction of food ingredients as antimicrobial preservation, chemical preservation of meat/fish, food ingredients containing natural extracts, etc., which can solve loss, strong fishy smell, and affect fish quality, etc. problem, to achieve a wide range of antibacterial effects, high freshness, and the effect of inhibiting the reproduction of bacteria
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Embodiment 1
[0034] Weigh 3.402g of gallic acid and 7.914g of n-decyl alcohol so that the molar mass ratio of gallic acid and n-decyl alcohol is 1:2.5, weigh 0.905g of immobilized lipase IM-100 as an enzymatic reaction catalyst, and measure 50mL of tert-butanol As a reaction medium, put it in an oil bath at 50°C for 13 hours, and use silica gel column chromatography to elute with petroleum ether and ethyl acetate at a volume ratio of 6:1 as the mobile phase, and the prepared product is GAC10.
Embodiment 2
[0036] Bifidobacterium cultures grown in MRS-C broth for 28 h at 37 °C were heated to 80 °C for 30 min to kill cells and prevent bacteriocin degradation by inactivating proteolytic enzymes. After cooling to room temperature, the medium was adjusted to pH 8.0 with sterile 1 M NaOH and stirred overnight at 4 °C to facilitate the adsorption of bacteriocin molecules on the surface of the producer cells. Target cells were obtained by centrifugation, washed three times with 0.1 M PBS buffer, and then suspended in 0.1 mM NaCl (pH 3.0) to desorb bacteriocin molecules from producer cells. The cell suspension was stirred overnight at 4°C. The supernatant was dialyzed at 4°C for 24 h using a 1.0 kDa cut-off membrane to obtain Bifidobacterium bacteriocin S.
Embodiment 3
[0038] The extraction method step of described mangosteen bark extract is as follows:
[0039] Take by weighing choline chloride 1.24g, ethylene glycol 5.585g, make choline chloride and ethylene glycol molar mass ratio be 1:2, by heating choline chloride as different hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA) to prepare a deep eutectic solvent. The mixture was placed in a water bath at 80° C. and stirred at 200 rpm until a uniform colorless liquid was obtained, which was the deep eutectic solvent. Mix 7.5mL of deep eutectic solvent and 2.5mL of ultrapure water in a closed container, put 2g of crushed mangosteen bark into the mixture, and place the closed container in a microwave extractor for extraction at 65°C for 20min. Mangosteen bark extract.
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