Colon bacillus outer membrane protein monoclonal antibody and preparation method and application thereof

A technology of monoclonal antibody and Escherichia coli, which is applied in the direction of anti-bacterial immunoglobulin, botany equipment and method, biochemical equipment and method, etc., can solve problems such as poor cross-immunity effect, difficulty in vaccine and drug prevention, etc., and achieve Extensive research and commercial use value, good neutralizing activity and protection efficiency, strong sensitivity effect
CN102584992AActive Publication Date: 2012-07-18兆丰华生物科技(南京)有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
兆丰华生物科技(南京)有限公司
Publication Date
2012-07-18

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Abstract

The invention belongs to the technical field of veterinary biology, and particularly relates to a colon bacillus outer membrane protein monoclonal antibody and a preparation method and an application thereof. The antibody is prepared by taking cloned, expressed and purified taking avian pathogenic escherichia coli 78 (APECO78) outer membrane protein as an antigen immune BALB / c mouse with a hybrid tumor technology. The antibody has high specificity and high sensitivity, and an experimental basis is laid for future rapid and specific detection of colon bacillus infection with an immunology method. Meanwhile, as proved by an experiment, the monoclonal antibody disclosed by the invention has high neutralizing activity and protecting efficiency on colon bacillus infection of the same type, and can be used for preventing colibacillosis.
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Description

technical field

[0001] The invention relates to an Escherichia coli outer membrane protein monoclonal antibody and a preparation method and application thereof, belonging to the field of biotechnology. Background technique

[0002] Avian colibacillosis is caused by avian pathogenic Escherichia coli, and is one of the most serious diseases to the poultry industry. At present, vaccines and antibacterial drugs are mainly used to prevent colibacillosis. However, there are many serotypes of Escherichia coli, and the cross-immunity effect between each serotype is not good, which brings certain difficulties to the prevention of vaccines and drugs.

[0003] Monoclonal antibody refers to the antibody against a single antigenic determinant produced by the progeny cells of a B cell differentiation and proliferation. Monoclonal antibody has strong pathogen specificity, does not affect normal tissues and cells, and is relatively safe. It is safe in bacteria and viruses. It plays an impo...

Claims

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