Convenient and simple expanding propagation method of arbuscular mycorrhizal fungus inoculant and expanding propagation structure thereof

Abstract

The invention discloses a convenient and simple expanding propagation method of arbuscular mycorrhizal fungus inoculant and an structure thereof. The expanding propagation method comprises the steps of a, refining seedling soil in a greenhouse through a 1mm mesh, and conducting steam sterilizing on the seedling soil at 121 DEG C for 2 hours to obtain a sterilization substrate; b, sending clover seeds into 0.1% sodium hypochlorite solution to sterilize for 2 min, and then using distilled water to wash the clover seeds to obtain the sterilized seeds; c, using a pasteurization solution to conduct sterilization on an expanding propagation greenhouse and a seedbed; putting the sterilization substrate doped with the arbuscular mycorrhizal fungus inoculant into the seedbed of the greenhouse, and adopting a broadcast sowing mode to sow the sterilized seeds; controlling temperature and humidity in the greenhouse at regular temperature, and cultivating for 3-4 months to obtain the arbuscular mycorrhizal fungus inoculant. According to the convenient and simple expanding propagation method of the arbuscular mycorrhizal fungus, expanding propagation replaces an existing pot expanding propagation with scalable greenhouse direct expanding propagation. The convenient and simple expanding propagation method of the arbuscular mycorrhizal fungus can increase the yield of the expanding propagation fungi, the substrate component is simple in structure, the materials are easy to obtain, and the expanding propagation method is low in cost and convenient for large-area expanding propagation.

Description

technical field [0001] The invention relates to a convenient and simple propagation method of arbuscular mycorrhizal fungal agent and its propagation structure, belonging to the technical field of agricultural application of AM fungi. Background technique [0002] Arbuscular mycorrhizal fungi ( Arbuscular mycorrhizal fungus ) referred to as AM fungus, which is widely distributed in nature. It can form mycorrhizae with more than 80% of plants on land, and can affect many metabolic processes of plants in different ways or ways. It not only affects the growth and development of plants, nutritional status, and resistance Reversibility, yield and quality play an important role, and can improve soil structure to a certain extent. [0003] Tobacco is one of the symbiotic plants of arbuscular mycorrhizal fungi. With the development of society, people pay more and more attention to the harm of smoking to the body, and the control of the application of chemical fertilizers and pestic...

AI Technical Summary

Problems solved by technology

[0005] At present, the screening method about AM fungal strains is to first isolate the spores of various AM fungi in the soil, then carry out the expansion of the bacterial strains, and then carry out the in vivo test after obtaining the AM fungal agent after the expansion. Pots and pots are often used as the multiplication substrate, and the multiplication substrate used is sand and soil at a ratio of 3:1. The entire project needs to be managed by professionals, and it cannot be popularized to farmers.

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