Suitable hydroponic device for root system culture of Agrobacterium rhizogenes transgenic plant

Abstract

The invention relates to a special hydroponics suitable for root system culture of Agrobacterium rhizogenes and its application, the device comprising a tank, a plant holder, wherein the plant holder comprises a vertical plate support, a clamping plate, and a plate-mounted frame body provided with a height adjustment groove and a chute provided with a combined upper-layer holding plate, a chute sealing strip and a light-shielding plate, the upper deck being provided with a plant gripping hole, the lower holding plate and the shading plate which are provided with corresponding root and stem holes. The tank is a rectangular box with a net length corresponding to the plant holder. The net width is an integral multiple of the width of the plant holder. The box contains an independent root zone corresponding to the root hole separated by a divider. The device configuration also includes an oxygen pump, a root pruning bracket and a pouring funnel. The device can be used for root system culture of Agrobacterium rhizogenes transgenic plants.

Description

technical field [0001] The invention relates to a special hydroponic device suitable for root system cultivation of Agrobacterium rhizogenes transgenic combined plants and a method for hydroponic transgenic hairy roots using the device. Background technique [0002] After Agrobacterium rhizogenes infects plants, it can induce plants to produce root nodules, and further form a large number of highly branched adventitious roots, usually called hairy roots, which have the characteristics of fast growth and high degree of differentiation. The hairy root can also regenerate into a complete plant under in vitro conditions. At this time, because the whole plant contains transgenic ingredients, it is a complete transgenic plant; if the hairy root infects the plant stem or root and forms a hairy root, then even All the common roots of the plant itself are cut off, and the hairy roots can also replace their functions to absorb water and nutrients, and the plants continue to grow and d...

AI Technical Summary

Problems solved by technology

This method is cumbersome to operate and is very time-consuming and laborious, and it is difficult to quickly cultivate large-scale

The difficulties are mainly manifested in the following aspects: 1) When pruning the non-transgenic hair roots, only one plant can be taken out, and after pruning one by one, they are put into test tubes and fixed in turn; 2) The test tubes are small in volume and need to change nutrients frequently every day solution, only a single tube can be changed at a time

3) It is necessary to pump air into each test tube separately, which is cumbersome to operate

4) Not suitable for fixation and cultivation of tall plants

5) The glass test tube is transparent, which is easy to breed algae and affect the experimental results

6) A large number of test tubes and test tube racks are required, which takes up a lot of space and is not easy to clean

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