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Method for disinfecting plumbago auriculata tissue culture explants

A technology of tissue culture and disinfection methods, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of unreasonable selection of disinfection methods, low survival rate, environmental pollution, etc., and achieve good disinfection effect and good effect , the effect of reducing environmental pollution

Inactive Publication Date: 2015-12-30
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing blue snowflake tissue culture technology, the choice of disinfection method is not reasonable enough, resulting in a low survival rate and easy to cause environmental pollution

Method used

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  • Method for disinfecting plumbago auriculata tissue culture explants
  • Method for disinfecting plumbago auriculata tissue culture explants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Explant selection: Select a 2-3 year-old fine blue snowflake single plant with obvious typical characteristics of the variety, strong growth potential, and no pests and diseases as the mother plant for explants, and choose robust growth, uniform internodes, and emerald green leaves The branches of the branch, cut the middle and upper part of the branch as an explant, and insert it into a beaker filled with clear water immediately after cutting;

[0023] (2) Explant pretreatment: cut the branches clipped in step (1) into 5-8cm segments, each segment is guaranteed to have 1-2 axillary buds, cut off larger leaves, and clean the resulting segment, the method is: Put the small section into a beaker, add washing powder, stir and rinse, then seal the mouth of the beaker with gauze, and rinse it under running water for 30 minutes. ;

[0024] (3) Disinfection of explants: soak the segment obtained in step (2) for 120 seconds with 75% ethanol, rinse 1-2 times with sterile wa...

Embodiment 2

[0026] (1) Explant selection: Select a 2-3 year-old fine blue snowflake single plant with obvious typical characteristics of the variety, strong growth potential, and no pests and diseases as the mother plant for explants, and choose robust growth, uniform internodes, and emerald green leaves The branches of the branch, cut the middle and upper part of the branch as an explant, and insert it into a beaker filled with clear water immediately after cutting;

[0027] (2) Explant pretreatment: cut the branches clipped in step (1) into 5-8cm segments, each segment is guaranteed to have 1-2 axillary buds, cut off larger leaves, and clean the resulting segment, the method is: Put the small pieces into a beaker, add washing powder, stir and rinse, then seal the mouth of the beaker with gauze, and rinse it under running water for 40 minutes. ;

[0028] (3) Disinfection of explants: soak the segment obtained in step (2) for 140 seconds with 75% ethanol, rinse 1-2 times with sterile wat...

Embodiment 3

[0030] (1) Explant selection: Select a 2-3 year-old fine blue snowflake single plant with obvious typical characteristics of the variety, strong growth potential, and no pests and diseases as the mother plant for explants, and choose robust growth, uniform internodes, and emerald green leaves The branches of the branch, cut the middle and upper part of the branch as an explant, and insert it into a beaker filled with clear water immediately after cutting;

[0031] (2) Explant pretreatment: cut the branches clipped in step (1) into 5-8cm segments, each segment is guaranteed to have 1-2 axillary buds, cut off larger leaves, and clean the resulting segment, the method is: Put the small section into a beaker, add washing powder, stir and rinse, then seal the mouth of the beaker with gauze, and rinse it under running water for 35 minutes. ;

[0032] (3) Disinfection of explants: soak the segment obtained in step (2) for 130 seconds with 75% ethanol, rinse 1-2 times with sterile wa...

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Abstract

The invention discloses a method for disinfecting plumbago auriculata tissue culture explants. The method includes steps of (1), selecting explants; (2), pre-treating the explants; (3), disinfecting the explants. Compared with the prior art, the method has the advantages that the method is easy to implement, the steps are scientific and reasonable, excellent disinfection effects can be realized without severe damage to the explants, the explant survival rate can be obviously increased, and environmental pollution can be effectively reduced.

Description

technical field [0001] The invention relates to a method for disinfecting blue snowflake tissue culture explants, belonging to the technical field of plant tissue culture. Background technique [0002] Blue Snowflake (Plumbago auriculata) is native to South Africa, also known as Blue Flower Dan, Blue Snow Dan, Blue Flower Rocky Pine, Blue Jasmine, etc. It belongs to the Plumbago auriculata family (Plumbago auriculata) and is a perennial evergreen shrub. Blue Snowflake grows vigorously, is heat-resistant, more resistant to high temperature and high humidity, less pests and diseases, easy to manage, and has a long viewing period. The leaves are emerald green, the flower color is elegant, and it gives people a cool feeling in the hot summer. It can be used as a potted plant to decorate the living room and balcony; mature plants with hanging branches are suitable for potting in large containers. It can also be planted on the edge of the forest or embellished with lawns. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 宗树斌王永平刘玉华顾立新陈少卿
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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