Method for disinfecting plumbago auriculata tissue culture explants
A technology of tissue culture and disinfection methods, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of unreasonable selection of disinfection methods, low survival rate, environmental pollution, etc., and achieve good disinfection effect and good effect , the effect of reducing environmental pollution
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Embodiment 1
[0022] (1) Explant selection: Select a 2-3 year-old fine blue snowflake single plant with obvious typical characteristics of the variety, strong growth potential, and no pests and diseases as the mother plant for explants, and choose robust growth, uniform internodes, and emerald green leaves The branches of the branch, cut the middle and upper part of the branch as an explant, and insert it into a beaker filled with clear water immediately after cutting;
[0023] (2) Explant pretreatment: cut the branches clipped in step (1) into 5-8cm segments, each segment is guaranteed to have 1-2 axillary buds, cut off larger leaves, and clean the resulting segment, the method is: Put the small section into a beaker, add washing powder, stir and rinse, then seal the mouth of the beaker with gauze, and rinse it under running water for 30 minutes. ;
[0024] (3) Disinfection of explants: soak the segment obtained in step (2) for 120 seconds with 75% ethanol, rinse 1-2 times with sterile wa...
Embodiment 2
[0026] (1) Explant selection: Select a 2-3 year-old fine blue snowflake single plant with obvious typical characteristics of the variety, strong growth potential, and no pests and diseases as the mother plant for explants, and choose robust growth, uniform internodes, and emerald green leaves The branches of the branch, cut the middle and upper part of the branch as an explant, and insert it into a beaker filled with clear water immediately after cutting;
[0027] (2) Explant pretreatment: cut the branches clipped in step (1) into 5-8cm segments, each segment is guaranteed to have 1-2 axillary buds, cut off larger leaves, and clean the resulting segment, the method is: Put the small pieces into a beaker, add washing powder, stir and rinse, then seal the mouth of the beaker with gauze, and rinse it under running water for 40 minutes. ;
[0028] (3) Disinfection of explants: soak the segment obtained in step (2) for 140 seconds with 75% ethanol, rinse 1-2 times with sterile wat...
Embodiment 3
[0030] (1) Explant selection: Select a 2-3 year-old fine blue snowflake single plant with obvious typical characteristics of the variety, strong growth potential, and no pests and diseases as the mother plant for explants, and choose robust growth, uniform internodes, and emerald green leaves The branches of the branch, cut the middle and upper part of the branch as an explant, and insert it into a beaker filled with clear water immediately after cutting;
[0031] (2) Explant pretreatment: cut the branches clipped in step (1) into 5-8cm segments, each segment is guaranteed to have 1-2 axillary buds, cut off larger leaves, and clean the resulting segment, the method is: Put the small section into a beaker, add washing powder, stir and rinse, then seal the mouth of the beaker with gauze, and rinse it under running water for 35 minutes. ;
[0032] (3) Disinfection of explants: soak the segment obtained in step (2) for 130 seconds with 75% ethanol, rinse 1-2 times with sterile wa...
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