Lonicera edulis tissue culturing method

A technology of honeysuckle and tissue culture, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problems of intensifying the contradiction between supply and demand of seedlings, affecting the development of the deep processing industry of indigo, and the promotion and popularization of fine varieties has not yet been completed, etc. To achieve the effect of stable genetic traits

Inactive Publication Date: 2009-11-11
FORESTRY RES INST OF HEILONGJIANG PROVINCE
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Problems solved by technology

However, no one has studied the rooting culture of Lonicerae lonicera, the tissue culture system has not been established, the popularization of fine varieties has not been completed, and the contradiction between supply and demand of seedlings has become increasingly serious, which has seriously affected the development of the deep processing industry of Lonicerae Lonicerae.

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  • Lonicera edulis tissue culturing method
  • Lonicera edulis tissue culturing method
  • Lonicera edulis tissue culturing method

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[0008] The twigs germinated from the dormant branches of Lonicera Lonicerae after hydroponics are used as explants, which are composed of the following four steps: (1) explant sterilization Firstly, the twigs extracted from the hydroponic culture are washed with washing powder solution by conventional methods. Washed, then sterilized with 0.1% mercuric chloride for 3 minutes, soaked in sterile water for 40 minutes; (2) inoculated shoots were inoculated on the first-generation medium under aseptic conditions, and the medium was conventionally cultured in MS 1mg / L of 6-benzyladenine (6-BA), 0.2mg / L of indolebutyric acid (IBA) were added to the base, pH 5.8, the culture temperature was 25±2°C, and the light cycle was 12h / d. The light intensity is 2000 lux, and after 1 month of cultivation, the next stage begins; (3) Proliferation Transfer the obtained seedlings to the proliferation medium for proliferation culture. With three hormones 6-BA, IBA, GA 3 Each of the two levels was s...

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Abstract

The invention belongs to the fields of plant tissue culturing technologies and forest cultivation and relates to a high-efficiency lonicera edulis propagation method. The method comprises four stages of explant sterilization, inoculation, multiplication and rootage. The method solves the important technical problems of microbody quick propagation and root induction of the lonicera edulis, achieves the aims of efficiently inducing explants to separate and propagate and guaranteeing that seedlings survive with high quality, preserves all good characters of a matrix, and ensures stable hereditary character. The method can form large qualities of good test-tube seedlings within a short period and is suitable for large-scale and industrial production.

Description

technical field [0001] The invention relates to a vegetative propagation method of berries in Northeast China, that is, a tissue culture method of Lonicera japonica. The invention belongs to the field of plant tissue culture technology and forest cultivation. Background technique [0002] Because of its potential nutritional value, medicinal value and commercial value, Lonicerae Lonicerae is a rare, pure natural, green edible berry in the world. It has a very broad domestic and foreign market. Further expand and develop Lonicera Lonicerae products, Has broad prospects. However, due to the unplanned and plundering picking, Lonicera indigo Lonicerae was seriously damaged, the natural repair ability was reduced, and the reserve and recoverable amount decreased sharply. Secondly, the purchase of indigo honeysuckle at Dongning Port in Heilongjiang Province has become popular, and the contradiction between supply and demand has become increasingly serious. [0003] The seedling...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 田新华郭树平卢慧颖
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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