Lonicera edulis tissue culturing method
A technology of honeysuckle and tissue culture, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problems of intensifying the contradiction between supply and demand of seedlings, affecting the development of the deep processing industry of indigo, and the promotion and popularization of fine varieties has not yet been completed, etc. To achieve the effect of stable genetic traits
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[0008] The twigs germinated from the dormant branches of Lonicera Lonicerae after hydroponics are used as explants, which are composed of the following four steps: (1) explant sterilization Firstly, the twigs extracted from the hydroponic culture are washed with washing powder solution by conventional methods. Washed, then sterilized with 0.1% mercuric chloride for 3 minutes, soaked in sterile water for 40 minutes; (2) inoculated shoots were inoculated on the first-generation medium under aseptic conditions, and the medium was conventionally cultured in MS 1mg / L of 6-benzyladenine (6-BA), 0.2mg / L of indolebutyric acid (IBA) were added to the base, pH 5.8, the culture temperature was 25±2°C, and the light cycle was 12h / d. The light intensity is 2000 lux, and after 1 month of cultivation, the next stage begins; (3) Proliferation Transfer the obtained seedlings to the proliferation medium for proliferation culture. With three hormones 6-BA, IBA, GA 3 Each of the two levels was s...
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