Method for cultivating potato virus-free seedlings

A cultivation method and potato technology, which are applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of large-scale popularization by farmers and high production costs, and achieve the improvement of the rate of potato setting per plant, the reduction of production costs, and the reduction of carbon emissions. The effect of content reduction

Inactive Publication Date: 2013-08-21
定西市旱作农业科研推广中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for cultivating virus-free potato seedlings, which solves the shortcomings of the traditional method, which has high production costs and is not easy to be popularized by farmers on a large scale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. The detoxified potato tissue culture seedlings were transferred to the MS medium with 50% carbendazim WP 5mg / L and 20g / L edible sugar at 0.15 Mpa / cm 2 Sterilize under pressure for 15-20 minutes, cool down and use this medium for subculture, and cultivate in the light culture room for 25 days;

[0018] 2. After hardening the seedlings, put the cutting seedlings in a mixture containing NAA 200mg / L + IBA 200mg / L + H 3 BO 3 Soak in 35mg / L + Tween-80 4mL / L + 95% alcohol 66mL / L CIP improved rooting agent mixture for 10min, then take out and insert;

[0019] 3. After the cuttings are planted, water and cover the shed to keep the relative humidity in the small shed at 90%. The ratio of N, P and K to the cutting seedlings before, during and after the growth is N:P 2 o 5 :K 2 O = 1: 0.57: 1.22, concentration is 2.0g / L, mass of total solute is 20g / m 2 Nutrient solution 4, 2, 2 times to ensure the nutritional requirements during the growth period;

[0020] 4. 30 days afte...

Embodiment 2

[0023] 1. The detoxified potato tissue culture seedlings were transferred to the MS medium with 50% carbendazim WP 5mg / L and 20g / L edible sugar at 0.15 Mpa / cm 2 Sterilize under pressure for 15-20 minutes, cool down and use this medium for subculture, and cultivate in the light culture room for 25 days;

[0024] 2. Choose robust and pollution-free tissue culture seedlings with a seedling age of about 25 days and a plant height of more than 5cm. After hardening the seedlings for 4 days, wash off the medium for planting. Contains NAA 200mg / L + IBA 200mg / L + H 3 BO 3 Soak in 35mg / L + Tween-80 4mL / L + 95% alcohol 66mL / L CIP improved rooting agent mixture for 10 minutes, then plant on the culture medium at a density of 250 plants / square meter, with a row spacing of 6.3cm and a plant spacing of 6.3cm middle;

[0025] 3. Pre-management: After the cuttings are planted, water and cover the shed to keep the relative humidity in the small shed at 90%. Water four times on the 12th day,...

Embodiment 3

[0031] One, transfer the potato tissue culture seedlings through detoxification into the used culture substrate of the previous stubble and apply 40% pentachloronitrobenzene powder, the amount of pentachloronitrobenzene powder is to account for 2% of the total recovery substrate, use This medium was subcultured and cultured in a light culture room for 25 days;

[0032] Two, follow-up method is the same as embodiment 2.

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Abstract

The invention discloses a method for cultivating potato virus-free seedlings, relates to the virus elimination and tissue culture techniques of plants, and belongs to the field of agricultural seedling breeding. The method is characterized by comprising the following steps of: transferring virus-free potato tissue culture seedlings into a MS culture medium to culture, wherein a bacteriostatic agent and sugar are added into the MS culture medium; and after the culturing is completed, soaking the seedlings into a CIP improved rooting agent, and carrying out cuttage planting and nutrient solution applying on the seedlings, and after the seedlings are mature, harvesting the seedlings. The method disclosed by the invention has the beneficial effects that through cultivating virus-free seedlings in a special culture medium, the contamination rate in the culture medium is reduced; then, the plant seedlings cultivated in the culture medium are subjected to cuttage, and before cuttage, the cuttage seedlings are placed in the CIP improved rooting agent, and nutrient solutions N, P and K are applied, so that the survival of virus-free seedlings is ensured, the potato growth amount and the expansion of tubers are promoted, thereby improving the yield; and the single-plant potato growth amount of breeder seeds is improved, so that the production cost of breeder seeds is reduced by 25%, thereby providing a reliable technical support for the industrialized development of potatoes.

Description

technical field [0001] The invention relates to plant detoxification and tissue culture technology, and belongs to the field of agricultural seedling breeding, in particular to a method for cultivating virus-free potato seedlings. Background technique [0002] potato( Solanum tuberosum L. ) is a dicotyledonous plant belonging to the Solanaceae Solanaceae. It has a wide planting area, the product is resistant to storage and transportation, and the price is moderate. It is recognized as a food and vegetable crop and the fourth largest food crop in the world. In 2007, the planting area in Gansu Province reached 9 million mu, and the total output exceeded 10 million tons for the first time, ranking among the top in the country. The potato industry is one of the most promising agricultural industries in the province. Especially in the arid central region, potato production is developing strongly. In Dingxi City, the potato industry is the most dominant industry, and it is also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘荣清袁安明徐祺昕张小静杨俊丰谭伟军陈自雄
Owner 定西市旱作农业科研推广中心
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