Methods of farming, domesticating, or cultivating
truffle fungi, including isolation and cultivation methods for producing pure
truffle fungi, or mini-truffles, typically a Tuber spp. or an Iamai spp. Examples of
truffle include Tuber melanosporum, Tuber magnatum, Tuber aestivum, Tuber uncinatum, Tuber borchii, Imaia spp., Tuber macrosporum, Tuber gibbosum, Tuber oregonense, and Tuber lyonii. The isolated pure truffle may then be cultivated on a
nutrient substrate, which may be, for example, a fruit, nut, grain, or a portion thereof, resulting in a truffle-flavored food product. Non-limiting examples of such substrates include rye, barley, lentil, wheat, rice, soybeans, pecan, hazelnut, pine nut, English walnut, coffee beans, mustard, cacao, sesame,
sunflower, grapes, blackberries, blueberries, cherries,
kiwi, mango, raspberries, and
huckleberries.