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Method for performing in-vivo induction on vaccinium australe tetraploid

A high-bush lingonberry and tetraploid technology, which is applied in the field of in vivo induction of southern high-bush bilberry tetraploids, can solve the problems of restricting the promotion of planting and small fruit, and achieve high health-care medicinal value, large single fruit, and vigorous growth Effect

Inactive Publication Date: 2015-06-24
朱远星
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Vaccinium australe (Vaccinium australe) has the advantages of strong stress resistance, fast growth and high yield, but the fruit is small which restricts its popularization and cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Rooting of tissue-cultured seedlings: Take 3-5 cm high and vigorously grown Vaccinium vaccinium tissue-cultured seedlings and inoculate them into the rooting medium to induce rooting. After inoculation, it was first cultivated in total darkness at 25°C for 12 days, and then placed in light for 14 hours a day, with a light intensity of 3500lx and a culture temperature of 25°C for 30 days to take root, and the rooting rate was 87.4%. The rooting medium described is 1 / 2MS+1.8mg / L NAA+1.0mg / L ABT 1 +25g / L sucrose+5.5g / L agar, the pH is 5.5.

[0017] (2) Seedling hardening and transplanting: open the bottle cap of the culture bottle of the test-tube seedling with a root length of 1-2 cm, harden the seedling outdoors under natural light for 3 days, take the test-tube seedling out of the culture bottle, wash off the root medium, and try not to damage The roots are planted in a slightly acidic substrate composed of humus:coconut peat:rice = 3:1:1 and planted in the field. ...

Embodiment 2

[0021] (1) Rooting of tissue-cultured seedlings: Take 3-5 cm high and vigorously grown Vaccinium vaccinium tissue-cultured seedlings and inoculate them into the rooting medium to induce rooting. After inoculation, it was first cultivated in total darkness at 27°C for 15 days, and then placed in light for 15 hours a day, with a light intensity of 4500lx and a culture temperature of 27°C for 28 days to take root, and the rooting rate was 90.46%. The rooting medium described is 1 / 2MS+2.5mg / L NAA+1.2mg / L ABT 1 +28g / L sucrose+5.5g / L agar, the pH is 5.8.

[0022] (2) Hardening and transplanting: open the bottle cap of the culture bottle of the test-tube seedling with a root length of 1-2 cm, and harden the seedling outdoors under natural light for 2 days. After 2 days, take the test-tube seedling out of the culture bottle, wash off the root medium, and try not to damage it. The roots are planted in a slightly acidic substrate composed of humus:coconut peat:rice = 3:1:1 and planted ...

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Abstract

The invention discloses a method for performing in-vivo induction on a vaccinium australe tetraploid. Vaccinium australe has the advantages of strong stress resistance, high growth speed and high yield; a tetraploid is taken as one of plant evolution routes and generally has the characteristics of hugeness, low fertility, strong growth, big fruits, thick and strong stems as well as strong stress resistance; and moreover, compared with conventional cross breeding, polyploid breeding is relatively short in cycle, and a new plant variety can be obtained relatively rapidly. According to the method disclosed by the invention, tissue culture seedlings and rooting seedlings are taken as materials, and a vaccinium australe plant with strong stress resistance, high quality, high yield and big single fruits can be obtained by performing tetraploid induction on the vaccinium australe tissue culture seedlings by using an in-vivo induction method so as to lay a foundation for cultivating new big fruit type cowberry blueberry varieties which are suitable for tropical and subtropical zones.

Description

technical field [0001] The invention relates to a method for cultivating plant polyploids in agricultural biotechnology, in particular to a method for inducing tetraploids of lingonberry in vivo. Background technique [0002] Bilberry belongs to Rhododendronaceae Vaccinium perennial deciduous or evergreen shrubs, the fruit is blue or red, the size of the fruit varies with different species, the pulp is soft and juicy, the flavor is mellow, rich in vitamins and rare in other fruits Its special nutritional ingredients have high health and medicinal value. Due to its unique flavor and nutritional and health value, its fruit and processed products are in short supply. Among the existing lingonberry cultivars, southern highbush bilberry is native to the subtropical southeastern United States, distributed in coastal and inland swamps, resistant to heat and humidity, cold, humid and warm climate conditions, suitable for subtropical climate conditions, It is mainly developed in th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 朱远星
Owner 朱远星
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