Extract of american cranberry and use thereof

An extract, cranberry technology, applied in the field of American cranberry extract and its use, can solve problems such as tedious treatment

Inactive Publication Date: 2009-10-14
DIANA NATURALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, the low proanthocyanidin dosage of these tablets forces the patient to take several tablets per day, making this treatment tedious

Method used

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  • Extract of american cranberry and use thereof
  • Extract of american cranberry and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: the method for preparing AFCANN9030 extract according to the present invention

[0044] American cranberries were placed in the aqueous phase solution in an amount of 1 volume of berries per 5 volumes of water. After grinding at a temperature of 25°C and solid-liquid separation by decantation, acetic acid was dissolved in an amount of 5 volumes of ethyl acetate per 1 volume of cranberries at a temperature of 20°C, a pH of 4.5, and a pressure of 1 atm. Ethyl ester was added to the aqueous phase and the resulting two phases were separated. Next, dehydration is performed at a reduced pressure of 0.1 to 0.5 atm and a temperature of 30 to 40° C., and then a second dehydration is performed at a pressure of 0.9 to 1.5 atm and a temperature of 145° C.

[0045] The obtained AFCANN9030 product is a purple powder.

Embodiment 2

[0046] Embodiment 2: Analysis of the proanthocyanidins in the obtained AFCANN9030 extract

[0047] The Bate-Smith method for analyzing proanthocyanidins modified by Porter was used (Bate-Smith E.C. Phytochemistry, 1973, 12, pp907-912 and Porter. L.J. et al., Phytochemistry, 1986, 25, 223-230).

[0048] The analytical reaction was carried out in two stages. First, flavan-3-ol polymers were hydrolyzed in butanol-HCl medium. Complete hydrolysis releases catechol and epicatechol-type monomers. The second analytical step consists in the Fe III These monomers are oxidized under the action. Oxidation products were anthocyanins and were quantified using a spectrophotometer at 541 nm.

[0049] By mixing 500 mL of 35% HCl, 500 mL of n-butanol and 150 mg of Fe 2 (SO 4 ) 3 To prepare Bate-Smith reagent.

[0050] 100 mg of American cranberry extract was dissolved in 100 mL of a 1 / 1 by weight mixture of demineralized water and ethanol. Mix 2 mL of this solution with 6 mL of Bate-...

Embodiment 3

[0055] Example 3: Characterization of the extract (AFCANN9030) of Example 1

[0056] Extract of Example 1

[0057] TPP: total polyphenols

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Abstract

The present invention relates to an extract of American cranberry, use thereof and a method of obtaining the extract. The extract contains at least 15 weight % of proanthocyanidin with respect to dry weight of extract and expressed by proanthocyanidin C1 valent weight. The proanthocyanidin-rich extract can be used as a dietary or nutraceutical preparation.

Description

technical field [0001] The present invention relates to an extract derived from bilberry (Vaccinium) and a method for obtaining the extract. This proanthocyanidin-rich extract can be used as diet preparation or nutritional preparation. Background technique [0002] Vaccinium macrocarpon Aiton (American cranberry) is a smaller creeping shrub not exceeding 30 cm that grows naturally only in eastern North America from the Carolinas in the United States to Canada. The plant thrives on the acidic soils of poor, moist peat bogs, moors or in coniferous forests. Its fruit is a small berry with a diameter of 10-20mm. When ripe, the small berries are bright red in color and have a slightly sour and astringent taste. [0003] American cranberries have been commonly consumed as food in North America for hundreds of years. It does not contain questionable levels of any component known to be toxic, and no incidents have been reported after consumption. Undesirable effects are rare at...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23L1/30A23L1/28A61K36/45A61P31/02A61P13/02A23L33/00
CPCA23L1/3002A23V2002/00A23L2/52A23L33/105A61P13/00A61P13/02A61P31/02A61P31/04A23V2250/21168A23V2250/21166A23V2250/2117A23V2250/606A23V2250/61A23V2250/628
Inventor 马蒂厄·贝纳尔克洛德·伊尼萨伊莎贝尔·鲁索
Owner DIANA NATURALS
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