The invention discloses a nutritional preparation for air force pilots and a preparation method thereof. The nutritional preparation comprises following components in parts by weight: 0.0004-0.0017 part of vitamin A, 0.0009-0.0022 part of vitamin B1, 0.0009-0.0022 part of vitamin B2, 0.0007-0.0022 part of vitamin B6, 0.000001-0.0000066 part of vitamin B12, 0.1-0.225 part of vitamin C, 0.000001-0.000002 part of vitamin D, 0.0076-0.018 part of vitamin E, 0.011-0.033 part of nicotinic acid, 0.028-0.175 part of iron nutrient supplement, 0.015-0.126 part of zinc nutrient supplement, 10-50 parts of protein substances, 1-10 parts of soybean peptide powder, 1-15 parts of fruit and vegetable powder, 1-10 parts of fructose, 5-40 parts of functional sugar, 10-50 parts of maltodextrin, 2-12 parts of powdered oil, 3-12 parts of nut powder, 0.11-0.14 part of taurine, 0-10 parts of a thickening agent and 0.1-1 part of an anti-caking agent. According to the nutritional preparation, three nutrients including carbohydrates, proteins and fat are scientifically matched and the vitamins and mineral substances are added on the basis to prepare the nutritional preparation.